Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing
Raw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-12-01
|
| Series: | Data in Brief |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340922009489 |
| _version_ | 1811206478211579904 |
|---|---|
| author | Rita Rahmeh Abrar Akbar Husam Alomirah Mohamed Kishk Abdulaziz Al-Ateeqi Salah Al-Milhm Anisha Shajan Batool Akbar Shafeah Al-Merri Mohammad Alotaibi Alfonso Esposito |
| author_facet | Rita Rahmeh Abrar Akbar Husam Alomirah Mohamed Kishk Abdulaziz Al-Ateeqi Salah Al-Milhm Anisha Shajan Batool Akbar Shafeah Al-Merri Mohammad Alotaibi Alfonso Esposito |
| author_sort | Rita Rahmeh |
| collection | DOAJ |
| description | Raw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three samples, and libraries were prepared using custom fusion primers of the 16S rRNA gene and sequenced on Illumina HiSeq 2500 platform. 16S rRNA gene sequences were aligned against the SILVA database SSU release 138. The high-throughput sequencing data are available at the NCBI database under the Bioproject PRJNA814013. This work describes camel milk's bacterial diversity among different geographical locations and seasons. The distribution of alpha diversity measures among camel milk sample groups collected from different geographical locations and seasons is presented. A significant effect of these parameters on camel milk's bacterial diversity was shown. Linear discriminant analysis (LefSe) showed significant differentially abundant bacteria at the phylum, class, order, family and genus level among the three locations and seasons. LefSe identified a total of 83 and 40 differentially abundant genera in the different geographical locations and seasons, respectively. More details about the bacterial composition of raw camel milk at the phylum and genus level can be found in research article [1]. These data can be used to compare the diversity of milk bacterial community between different milk producing species and camels from different parts of the world. Besides, these findings will contribute to our understanding of the camel microbiome structure and might be useful for designing an appropriate control program in the camel dairy herd. The data described in this article are available in Mendeley Data [2]. |
| first_indexed | 2024-04-12T03:48:11Z |
| format | Article |
| id | doaj.art-06a1c0447d614aa8b092a84e36a11b10 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-04-12T03:48:11Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-06a1c0447d614aa8b092a84e36a11b102022-12-22T03:49:03ZengElsevierData in Brief2352-34092022-12-0145108744Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencingRita Rahmeh0Abrar Akbar1Husam Alomirah2Mohamed Kishk3Abdulaziz Al-Ateeqi4Salah Al-Milhm5Anisha Shajan6Batool Akbar7Shafeah Al-Merri8Mohammad Alotaibi9Alfonso Esposito10Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, Kuwait; Corresponding author.Environment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitEnvironment and Life Sciences Research Centre, Kuwait Institute for Scientific Research, KuwaitInternational Centre for Genetic Engineering and Biotechnology, Trieste, ItalyRaw camel milk samples were collected from three geographical locations (south, north and middle Kuwait) during two seasons. Next generation sequencing of the V3-V4 regions of the 16S rRNA gene was used to analyze the bacterial community in camel milk. DNA was extracted from one hundred thirty-three samples, and libraries were prepared using custom fusion primers of the 16S rRNA gene and sequenced on Illumina HiSeq 2500 platform. 16S rRNA gene sequences were aligned against the SILVA database SSU release 138. The high-throughput sequencing data are available at the NCBI database under the Bioproject PRJNA814013. This work describes camel milk's bacterial diversity among different geographical locations and seasons. The distribution of alpha diversity measures among camel milk sample groups collected from different geographical locations and seasons is presented. A significant effect of these parameters on camel milk's bacterial diversity was shown. Linear discriminant analysis (LefSe) showed significant differentially abundant bacteria at the phylum, class, order, family and genus level among the three locations and seasons. LefSe identified a total of 83 and 40 differentially abundant genera in the different geographical locations and seasons, respectively. More details about the bacterial composition of raw camel milk at the phylum and genus level can be found in research article [1]. These data can be used to compare the diversity of milk bacterial community between different milk producing species and camels from different parts of the world. Besides, these findings will contribute to our understanding of the camel microbiome structure and might be useful for designing an appropriate control program in the camel dairy herd. The data described in this article are available in Mendeley Data [2].http://www.sciencedirect.com/science/article/pii/S2352340922009489Camel milkMetagenomicsMicrobial diversitySeasonGeographical locations |
| spellingShingle | Rita Rahmeh Abrar Akbar Husam Alomirah Mohamed Kishk Abdulaziz Al-Ateeqi Salah Al-Milhm Anisha Shajan Batool Akbar Shafeah Al-Merri Mohammad Alotaibi Alfonso Esposito Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing Data in Brief Camel milk Metagenomics Microbial diversity Season Geographical locations |
| title | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
| title_full | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
| title_fullStr | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
| title_full_unstemmed | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
| title_short | Data on microbial diversity of camel milk microbiota determined by 16S rRNA gene sequencing |
| title_sort | data on microbial diversity of camel milk microbiota determined by 16s rrna gene sequencing |
| topic | Camel milk Metagenomics Microbial diversity Season Geographical locations |
| url | http://www.sciencedirect.com/science/article/pii/S2352340922009489 |
| work_keys_str_mv | AT ritarahmeh dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT abrarakbar dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT husamalomirah dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT mohamedkishk dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT abdulazizalateeqi dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT salahalmilhm dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT anishashajan dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT batoolakbar dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT shafeahalmerri dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT mohammadalotaibi dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing AT alfonsoesposito dataonmicrobialdiversityofcamelmilkmicrobiotadeterminedby16srrnagenesequencing |