Hi-C analysis of genomic contacts revealed karyotype abnormalities in chicken HD3 cell line
Abstract Background Karyotype abnormalities are frequent in immortalized continuous cell lines either transformed or derived from primary tumors. Chromosomal rearrangements can cause dramatic changes in gene expression and affect cellular phenotype and behavior during in vitro culture. Structural va...
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| Format: | Article |
| Language: | English |
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BMC
2023-02-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09158-y |
| _version_ | 1811166038895624192 |
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| author | A. Maslova V. Plotnikov M. Nuriddinov M. Gridina V. Fishman A. Krasikova |
| author_facet | A. Maslova V. Plotnikov M. Nuriddinov M. Gridina V. Fishman A. Krasikova |
| author_sort | A. Maslova |
| collection | DOAJ |
| description | Abstract Background Karyotype abnormalities are frequent in immortalized continuous cell lines either transformed or derived from primary tumors. Chromosomal rearrangements can cause dramatic changes in gene expression and affect cellular phenotype and behavior during in vitro culture. Structural variations of chromosomes in many continuous mammalian cell lines are well documented, but chromosome aberrations in cell lines from other vertebrate models often remain understudied. The chicken LSCC-HD3 cell line (HD3), generated from erythroid precursors, was used as an avian model for erythroid differentiation and lineage-specific gene expression. However, karyotype abnormalities in the HD3 cell line were not assessed. In the present study, we applied high-throughput chromosome conformation capture to analyze 3D genome organization and to detect chromosome rearrangements in the HD3 cell line. Results We obtained Hi-C maps of genomic interactions for the HD3 cell line and compared A/B compartments and topologically associating domains between HD3 and several other cell types. By analysis of contact patterns in the Hi-C maps of HD3 cells, we identified more than 25 interchromosomal translocations of regions ≥ 200 kb on both micro- and macrochromosomes. We classified most of the observed translocations as unbalanced, leading to the formation of heteromorphic chromosomes. In many cases of microchromosome rearrangements, an entire microchromosome together with other macro- and microchromosomes participated in the emergence of a derivative chromosome, resembling “chromosomal fusions'' between acrocentric microchromosomes. Intrachromosomal inversions, deletions and duplications were also detected in HD3 cells. Several of the identified simple and complex chromosomal rearrangements, such as between GGA2 and GGA1qter; GGA5, GGA4p and GGA7p; GGA4q, GGA6 and GGA19; and duplication of the sex chromosome GGAW, were confirmed by FISH. Conclusions In the erythroid progenitor HD3 cell line, in contrast to mature and immature erythrocytes, the genome is organized into distinct topologically associating domains. The HD3 cell line has a severely rearranged karyotype with most of the chromosomes engaged in translocations and can be used in studies of genome structure–function relationships. Hi-C proved to be a reliable tool for simultaneous assessment of the spatial genome organization and chromosomal aberrations in karyotypes of birds with a large number of microchromosomes. |
| first_indexed | 2024-04-10T15:45:56Z |
| format | Article |
| id | doaj.art-06a2aa03dbcc4a12a4cc462120665bba |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-10T15:45:56Z |
| publishDate | 2023-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-06a2aa03dbcc4a12a4cc462120665bba2023-02-12T12:05:19ZengBMCBMC Genomics1471-21642023-02-0124111410.1186/s12864-023-09158-yHi-C analysis of genomic contacts revealed karyotype abnormalities in chicken HD3 cell lineA. Maslova0V. Plotnikov1M. Nuriddinov2M. Gridina3V. Fishman4A. Krasikova5Saint Petersburg State UniversitySaint Petersburg State UniversityInstitute of Cytology and Genetics SB RASInstitute of Cytology and Genetics SB RASInstitute of Cytology and Genetics SB RASSaint Petersburg State UniversityAbstract Background Karyotype abnormalities are frequent in immortalized continuous cell lines either transformed or derived from primary tumors. Chromosomal rearrangements can cause dramatic changes in gene expression and affect cellular phenotype and behavior during in vitro culture. Structural variations of chromosomes in many continuous mammalian cell lines are well documented, but chromosome aberrations in cell lines from other vertebrate models often remain understudied. The chicken LSCC-HD3 cell line (HD3), generated from erythroid precursors, was used as an avian model for erythroid differentiation and lineage-specific gene expression. However, karyotype abnormalities in the HD3 cell line were not assessed. In the present study, we applied high-throughput chromosome conformation capture to analyze 3D genome organization and to detect chromosome rearrangements in the HD3 cell line. Results We obtained Hi-C maps of genomic interactions for the HD3 cell line and compared A/B compartments and topologically associating domains between HD3 and several other cell types. By analysis of contact patterns in the Hi-C maps of HD3 cells, we identified more than 25 interchromosomal translocations of regions ≥ 200 kb on both micro- and macrochromosomes. We classified most of the observed translocations as unbalanced, leading to the formation of heteromorphic chromosomes. In many cases of microchromosome rearrangements, an entire microchromosome together with other macro- and microchromosomes participated in the emergence of a derivative chromosome, resembling “chromosomal fusions'' between acrocentric microchromosomes. Intrachromosomal inversions, deletions and duplications were also detected in HD3 cells. Several of the identified simple and complex chromosomal rearrangements, such as between GGA2 and GGA1qter; GGA5, GGA4p and GGA7p; GGA4q, GGA6 and GGA19; and duplication of the sex chromosome GGAW, were confirmed by FISH. Conclusions In the erythroid progenitor HD3 cell line, in contrast to mature and immature erythrocytes, the genome is organized into distinct topologically associating domains. The HD3 cell line has a severely rearranged karyotype with most of the chromosomes engaged in translocations and can be used in studies of genome structure–function relationships. Hi-C proved to be a reliable tool for simultaneous assessment of the spatial genome organization and chromosomal aberrations in karyotypes of birds with a large number of microchromosomes.https://doi.org/10.1186/s12864-023-09158-yHD3A/B compartmentsAvian karyotypeChicken cellsChromosome rearrangementChromosome translocation |
| spellingShingle | A. Maslova V. Plotnikov M. Nuriddinov M. Gridina V. Fishman A. Krasikova Hi-C analysis of genomic contacts revealed karyotype abnormalities in chicken HD3 cell line BMC Genomics HD3 A/B compartments Avian karyotype Chicken cells Chromosome rearrangement Chromosome translocation |
| title | Hi-C analysis of genomic contacts revealed karyotype abnormalities in chicken HD3 cell line |
| title_full | Hi-C analysis of genomic contacts revealed karyotype abnormalities in chicken HD3 cell line |
| title_fullStr | Hi-C analysis of genomic contacts revealed karyotype abnormalities in chicken HD3 cell line |
| title_full_unstemmed | Hi-C analysis of genomic contacts revealed karyotype abnormalities in chicken HD3 cell line |
| title_short | Hi-C analysis of genomic contacts revealed karyotype abnormalities in chicken HD3 cell line |
| title_sort | hi c analysis of genomic contacts revealed karyotype abnormalities in chicken hd3 cell line |
| topic | HD3 A/B compartments Avian karyotype Chicken cells Chromosome rearrangement Chromosome translocation |
| url | https://doi.org/10.1186/s12864-023-09158-y |
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