Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cells
Purpose: The aim of this study was to examine the effect of 17β-estradiol on Benzo(e)pyrene [B(e)P]-induced toxicity in ARPE-19 cells. Methods: We pretreated ARPE-19 cells with 20 nM and 40 nM 17β-estradiol for 6 hours, followed by addition of 300 μM B(e)P for additional 24 hours. Cell viability was...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Knowledge E
2018-01-01
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| Series: | Journal of Ophthalmic & Vision Research |
| Subjects: | |
| Online Access: | http://www.jovr.org/article.asp?issn=2008-322X;year=2018;volume=13;issue=4;spage=419;epage=425;aulast=Sapkal |
| _version_ | 1811193991844069376 |
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| author | Ashish U Sapkal Sonali Nashine Saffar Mansoor Vishal R Sharma Claudio A Ramirez Rafael Z Migon Navin K Gupta Marilyn Chwa Baruch D Kuppermann Cristina Kenney |
| author_facet | Ashish U Sapkal Sonali Nashine Saffar Mansoor Vishal R Sharma Claudio A Ramirez Rafael Z Migon Navin K Gupta Marilyn Chwa Baruch D Kuppermann Cristina Kenney |
| author_sort | Ashish U Sapkal |
| collection | DOAJ |
| description | Purpose: The aim of this study was to examine the effect of 17β-estradiol on Benzo(e)pyrene [B(e)P]-induced toxicity in ARPE-19 cells.
Methods: We pretreated ARPE-19 cells with 20 nM and 40 nM 17β-estradiol for 6 hours, followed by addition of 300 μM B(e)P for additional 24 hours. Cell viability was measured using Trypan blue dye-exclusion assay. JC-1 assay was performed to measure mitochondrial membrane potential (ΔΨm). For a quantitative estimation of cell death, apoptotic markers such as caspase-3/7, caspase-9, and caspase-12 were measured.
Results: Our results demonstrated that when treated with B(e)P, the viability and ΔΨm of ARPE-19 cells declined by 25% and 63%, respectively (P < 0.05). However, pretreating with 17β-estradiol increased the viability of ARPE-19 cells by 21% (20 nM) and 10% (40 nM) (P < 0.05). Furthermore, the significantly reduced ΔΨm in βE+B(e)P treated cells ARPE-19 cells was restored by pre-treatment with 17β-estradiol- ΔΨm was increased by 177% (20 nM) and 158% (40 nM) (P < 0.05). We further observed a significant up-regulation in the activity of Caspases-3/7, -9, and -12 in B(e)P-treated ARPE-19 cells. However, 17β-estradiol treatment significantly reduced the activity of all apoptotic markers (P < 0.05).
Conclusion: In conclusion, our results demonstrate that 17β-estradiol protects ARPE-19 cells against B(e)P-induced toxicity by decreasing apoptosis, preventing cell death, and restoring mitochondrial membrane potential. |
| first_indexed | 2024-04-12T00:19:45Z |
| format | Article |
| id | doaj.art-06a440388809425c9a4d812276df9c44 |
| institution | Directory Open Access Journal |
| issn | 2008-322X |
| language | English |
| last_indexed | 2024-04-12T00:19:45Z |
| publishDate | 2018-01-01 |
| publisher | Knowledge E |
| record_format | Article |
| series | Journal of Ophthalmic & Vision Research |
| spelling | doaj.art-06a440388809425c9a4d812276df9c442022-12-22T03:55:45ZengKnowledge EJournal of Ophthalmic & Vision Research2008-322X2018-01-0113441942510.4103/jovr.jovr_16_18Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cellsAshish U SapkalSonali NashineSaffar MansoorVishal R SharmaClaudio A RamirezRafael Z MigonNavin K GuptaMarilyn ChwaBaruch D KuppermannCristina KenneyPurpose: The aim of this study was to examine the effect of 17β-estradiol on Benzo(e)pyrene [B(e)P]-induced toxicity in ARPE-19 cells. Methods: We pretreated ARPE-19 cells with 20 nM and 40 nM 17β-estradiol for 6 hours, followed by addition of 300 μM B(e)P for additional 24 hours. Cell viability was measured using Trypan blue dye-exclusion assay. JC-1 assay was performed to measure mitochondrial membrane potential (ΔΨm). For a quantitative estimation of cell death, apoptotic markers such as caspase-3/7, caspase-9, and caspase-12 were measured. Results: Our results demonstrated that when treated with B(e)P, the viability and ΔΨm of ARPE-19 cells declined by 25% and 63%, respectively (P < 0.05). However, pretreating with 17β-estradiol increased the viability of ARPE-19 cells by 21% (20 nM) and 10% (40 nM) (P < 0.05). Furthermore, the significantly reduced ΔΨm in βE+B(e)P treated cells ARPE-19 cells was restored by pre-treatment with 17β-estradiol- ΔΨm was increased by 177% (20 nM) and 158% (40 nM) (P < 0.05). We further observed a significant up-regulation in the activity of Caspases-3/7, -9, and -12 in B(e)P-treated ARPE-19 cells. However, 17β-estradiol treatment significantly reduced the activity of all apoptotic markers (P < 0.05). Conclusion: In conclusion, our results demonstrate that 17β-estradiol protects ARPE-19 cells against B(e)P-induced toxicity by decreasing apoptosis, preventing cell death, and restoring mitochondrial membrane potential.http://www.jovr.org/article.asp?issn=2008-322X;year=2018;volume=13;issue=4;spage=419;epage=425;aulast=Sapkal17β-Estradiol; Benzo(e)pyrene; Age-Related Macular Degeneration; Apoptosis; Retinal Pigment Epithelial Cells; Smoking |
| spellingShingle | Ashish U Sapkal Sonali Nashine Saffar Mansoor Vishal R Sharma Claudio A Ramirez Rafael Z Migon Navin K Gupta Marilyn Chwa Baruch D Kuppermann Cristina Kenney Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cells Journal of Ophthalmic & Vision Research 17β-Estradiol; Benzo(e)pyrene; Age-Related Macular Degeneration; Apoptosis; Retinal Pigment Epithelial Cells; Smoking |
| title | Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cells |
| title_full | Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cells |
| title_fullStr | Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cells |
| title_full_unstemmed | Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cells |
| title_short | Protective effects of 17β-estradiol on Benzo(e)pyrene[B(e)P]-induced toxicity in ARPE-19 cells |
| title_sort | protective effects of 17β estradiol on benzo e pyrene b e p induced toxicity in arpe 19 cells |
| topic | 17β-Estradiol; Benzo(e)pyrene; Age-Related Macular Degeneration; Apoptosis; Retinal Pigment Epithelial Cells; Smoking |
| url | http://www.jovr.org/article.asp?issn=2008-322X;year=2018;volume=13;issue=4;spage=419;epage=425;aulast=Sapkal |
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