Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica
To reveal the mechanism of anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica, renal fibrosis was induced with unilateral ureteral obstruction (UUO) and then treated with an n-butanol extract (BUT) from Amygdalus mongolica (Rosaceae). Sixty male Sprague-Dawley rats were ran...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sciendo
2022-09-01
|
| Series: | Acta Pharmaceutica |
| Subjects: | |
| Online Access: | https://doi.org/10.2478/acph-2022-0023 |
| _version_ | 1818240099479977984 |
|---|---|
| author | Gao Chen Chang Hong Zhou Hong-Bing Liu Qing Bai Ying-Chun Liu Quan-Li Bai Wan-Fu Shi Song-Li |
| author_facet | Gao Chen Chang Hong Zhou Hong-Bing Liu Qing Bai Ying-Chun Liu Quan-Li Bai Wan-Fu Shi Song-Li |
| author_sort | Gao Chen |
| collection | DOAJ |
| description | To reveal the mechanism of anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica, renal fibrosis was induced with unilateral ureteral obstruction (UUO) and then treated with an n-butanol extract (BUT) from Amygdalus mongolica (Rosaceae). Sixty male Sprague-Dawley rats were randomly divided into the sham-operated, renal fibrosis (RF) model, benazepril hydrochloride-treated model (1.5 mg kg−1) and BUT-treated (1.75, 1.5 and 1.25 g kg−1) groups and the respective drugs were administered intragastrically for 21 days. Related biochemical indices in rat serum were determined and histopathological morphology observed. Serum metabolomics was assessed with HPLC-Q-TOF-MS. The BUT reduced levels of blood urea nitrogen, serum creatinine and albumin and lowered the content of malondialdehyde and hydroxyproline in tissues. The activity of superoxide dismutase in tissues was increased and an improvement in the severity of RF was observed. Sixteen possible biomarkers were identified by metabolomic analysis and six key metabolic pathways, including the TCA cycle and tyrosine metabolism, were analyzed. After treatment with the extract, 8, 12 and 9 possible biomarkers could be detected in the high-, medium- and low-dose groups, respectively. Key biomarkers of RF, identified using metabolomics, were most affected by the medium dose. A. mongolica BUT extract displays a protective effect on RF in rats and should be investigated as a candidate drug for the treatment of the disease. |
| first_indexed | 2024-12-12T13:08:03Z |
| format | Article |
| id | doaj.art-06a51d93cea546338861588fee6541a3 |
| institution | Directory Open Access Journal |
| issn | 1846-9558 |
| language | English |
| last_indexed | 2024-12-12T13:08:03Z |
| publishDate | 2022-09-01 |
| publisher | Sciendo |
| record_format | Article |
| series | Acta Pharmaceutica |
| spelling | doaj.art-06a51d93cea546338861588fee6541a32022-12-22T00:23:36ZengSciendoActa Pharmaceutica1846-95582022-09-0172343744810.2478/acph-2022-0023Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolicaGao Chen0Chang Hong1Zhou Hong-Bing2Liu Qing3Bai Ying-Chun4Liu Quan-Li5Bai Wan-Fu6Shi Song-Li7Department of Pharmacy, Baotou Medical College, Baotou, Inner MongoliaChina, 014040Department of Pharmacy, Baotou Medical College, Baotou, Inner MongoliaChina, 014040Department of Pharmacy, Baotou Medical College, Baotou, Inner MongoliaChina, 014040Department of Pharmacy, Baotou Medical College, Baotou, Inner MongoliaChina, 014040Department of Pharmacy, Baotou Medical College, Baotou, Inner MongoliaChina, 014040Department of Pharmacy, Baotou Medical College, Baotou, Inner MongoliaChina, 014040Department of Pharmacy, Baotou Medical College, Baotou, Inner MongoliaChina, 014040Department of Pharmacy, Baotou Medical College, Baotou, Inner MongoliaChina, 014040To reveal the mechanism of anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica, renal fibrosis was induced with unilateral ureteral obstruction (UUO) and then treated with an n-butanol extract (BUT) from Amygdalus mongolica (Rosaceae). Sixty male Sprague-Dawley rats were randomly divided into the sham-operated, renal fibrosis (RF) model, benazepril hydrochloride-treated model (1.5 mg kg−1) and BUT-treated (1.75, 1.5 and 1.25 g kg−1) groups and the respective drugs were administered intragastrically for 21 days. Related biochemical indices in rat serum were determined and histopathological morphology observed. Serum metabolomics was assessed with HPLC-Q-TOF-MS. The BUT reduced levels of blood urea nitrogen, serum creatinine and albumin and lowered the content of malondialdehyde and hydroxyproline in tissues. The activity of superoxide dismutase in tissues was increased and an improvement in the severity of RF was observed. Sixteen possible biomarkers were identified by metabolomic analysis and six key metabolic pathways, including the TCA cycle and tyrosine metabolism, were analyzed. After treatment with the extract, 8, 12 and 9 possible biomarkers could be detected in the high-, medium- and low-dose groups, respectively. Key biomarkers of RF, identified using metabolomics, were most affected by the medium dose. A. mongolica BUT extract displays a protective effect on RF in rats and should be investigated as a candidate drug for the treatment of the disease.https://doi.org/10.2478/acph-2022-0023amygdalus mongolicarenal fibrosisprotective effectmetabolomicsmechanism |
| spellingShingle | Gao Chen Chang Hong Zhou Hong-Bing Liu Qing Bai Ying-Chun Liu Quan-Li Bai Wan-Fu Shi Song-Li Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica Acta Pharmaceutica amygdalus mongolica renal fibrosis protective effect metabolomics mechanism |
| title | Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica |
| title_full | Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica |
| title_fullStr | Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica |
| title_full_unstemmed | Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica |
| title_short | Metabolomics reveal the mechanism for anti-renal fibrosis effects of an n-butanol extract from Amygdalus mongolica |
| title_sort | metabolomics reveal the mechanism for anti renal fibrosis effects of an n butanol extract from amygdalus mongolica |
| topic | amygdalus mongolica renal fibrosis protective effect metabolomics mechanism |
| url | https://doi.org/10.2478/acph-2022-0023 |
| work_keys_str_mv | AT gaochen metabolomicsrevealthemechanismforantirenalfibrosiseffectsofannbutanolextractfromamygdalusmongolica AT changhong metabolomicsrevealthemechanismforantirenalfibrosiseffectsofannbutanolextractfromamygdalusmongolica AT zhouhongbing metabolomicsrevealthemechanismforantirenalfibrosiseffectsofannbutanolextractfromamygdalusmongolica AT liuqing metabolomicsrevealthemechanismforantirenalfibrosiseffectsofannbutanolextractfromamygdalusmongolica AT baiyingchun metabolomicsrevealthemechanismforantirenalfibrosiseffectsofannbutanolextractfromamygdalusmongolica AT liuquanli metabolomicsrevealthemechanismforantirenalfibrosiseffectsofannbutanolextractfromamygdalusmongolica AT baiwanfu metabolomicsrevealthemechanismforantirenalfibrosiseffectsofannbutanolextractfromamygdalusmongolica AT shisongli metabolomicsrevealthemechanismforantirenalfibrosiseffectsofannbutanolextractfromamygdalusmongolica |