Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by <it>Paenibacillus elgii</it>
<p>Abstract</p> <p>Background</p> <p>Pelgipeptin, a potent antibacterial and antifungal agent, is a non-ribosomally synthesised lipopeptide antibiotic. This compound consists of a β-hydroxy fatty acid and nine amino acids. To date, there is no information about its bios...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2012-09-01
|
| Series: | BMC Microbiology |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2180/12/197 |
| _version_ | 1811266141113286656 |
|---|---|
| author | Qian Chao-Dong Liu Tian-Zhe Zhou Shuang-Lin Ding Rui Zhao Wen-Peng Li Ou Wu Xue-Chang |
| author_facet | Qian Chao-Dong Liu Tian-Zhe Zhou Shuang-Lin Ding Rui Zhao Wen-Peng Li Ou Wu Xue-Chang |
| author_sort | Qian Chao-Dong |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Pelgipeptin, a potent antibacterial and antifungal agent, is a non-ribosomally synthesised lipopeptide antibiotic. This compound consists of a β-hydroxy fatty acid and nine amino acids. To date, there is no information about its biosynthetic pathway.</p> <p>Results</p> <p>A potential pelgipeptin synthetase gene cluster (<it>plp</it>) was identified from <it>Paenibacillus elgii</it> B69 through genome analysis. The gene cluster spans 40.8 kb with eight open reading frames. Among the genes in this cluster, three large genes, <it>plpD</it>, <it>plpE</it>, and <it>plpF,</it> were shown to encode non-ribosomal peptide synthetases (NRPSs), with one, seven, and one module(s), respectively. Bioinformatic analysis of the substrate specificity of all nine adenylation domains indicated that the sequence of the NRPS modules is well collinear with the order of amino acids in pelgipeptin. Additional biochemical analysis of four recombinant adenylation domains (PlpD A1, PlpE A1, PlpE A3, and PlpF A1) provided further evidence that the <it>plp</it> gene cluster involved in pelgipeptin biosynthesis.</p> <p>Conclusions</p> <p>In this study, a gene cluster (<it>plp</it>) responsible for the biosynthesis of pelgipeptin was identified from the genome sequence of <it>Paenibacillus elgii</it> B69. The identification of the <it>plp</it> gene cluster provides an opportunity to develop novel lipopeptide antibiotics by genetic engineering.</p> |
| first_indexed | 2024-04-12T20:36:42Z |
| format | Article |
| id | doaj.art-06a55d6ae66944c29a2d4e5cf08043a9 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-04-12T20:36:42Z |
| publishDate | 2012-09-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-06a55d6ae66944c29a2d4e5cf08043a92022-12-22T03:17:33ZengBMCBMC Microbiology1471-21802012-09-0112119710.1186/1471-2180-12-197Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by <it>Paenibacillus elgii</it>Qian Chao-DongLiu Tian-ZheZhou Shuang-LinDing RuiZhao Wen-PengLi OuWu Xue-Chang<p>Abstract</p> <p>Background</p> <p>Pelgipeptin, a potent antibacterial and antifungal agent, is a non-ribosomally synthesised lipopeptide antibiotic. This compound consists of a β-hydroxy fatty acid and nine amino acids. To date, there is no information about its biosynthetic pathway.</p> <p>Results</p> <p>A potential pelgipeptin synthetase gene cluster (<it>plp</it>) was identified from <it>Paenibacillus elgii</it> B69 through genome analysis. The gene cluster spans 40.8 kb with eight open reading frames. Among the genes in this cluster, three large genes, <it>plpD</it>, <it>plpE</it>, and <it>plpF,</it> were shown to encode non-ribosomal peptide synthetases (NRPSs), with one, seven, and one module(s), respectively. Bioinformatic analysis of the substrate specificity of all nine adenylation domains indicated that the sequence of the NRPS modules is well collinear with the order of amino acids in pelgipeptin. Additional biochemical analysis of four recombinant adenylation domains (PlpD A1, PlpE A1, PlpE A3, and PlpF A1) provided further evidence that the <it>plp</it> gene cluster involved in pelgipeptin biosynthesis.</p> <p>Conclusions</p> <p>In this study, a gene cluster (<it>plp</it>) responsible for the biosynthesis of pelgipeptin was identified from the genome sequence of <it>Paenibacillus elgii</it> B69. The identification of the <it>plp</it> gene cluster provides an opportunity to develop novel lipopeptide antibiotics by genetic engineering.</p>http://www.biomedcentral.com/1471-2180/12/197Non-ribosomal peptideBiosynthesisGene clusterAntimicrobial agent |
| spellingShingle | Qian Chao-Dong Liu Tian-Zhe Zhou Shuang-Lin Ding Rui Zhao Wen-Peng Li Ou Wu Xue-Chang Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by <it>Paenibacillus elgii</it> BMC Microbiology Non-ribosomal peptide Biosynthesis Gene cluster Antimicrobial agent |
| title | Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by <it>Paenibacillus elgii</it> |
| title_full | Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by <it>Paenibacillus elgii</it> |
| title_fullStr | Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by <it>Paenibacillus elgii</it> |
| title_full_unstemmed | Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by <it>Paenibacillus elgii</it> |
| title_short | Identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by <it>Paenibacillus elgii</it> |
| title_sort | identification and functional analysis of gene cluster involvement in biosynthesis of the cyclic lipopeptide antibiotic pelgipeptin produced by it paenibacillus elgii it |
| topic | Non-ribosomal peptide Biosynthesis Gene cluster Antimicrobial agent |
| url | http://www.biomedcentral.com/1471-2180/12/197 |
| work_keys_str_mv | AT qianchaodong identificationandfunctionalanalysisofgeneclusterinvolvementinbiosynthesisofthecycliclipopeptideantibioticpelgipeptinproducedbyitpaenibacilluselgiiit AT liutianzhe identificationandfunctionalanalysisofgeneclusterinvolvementinbiosynthesisofthecycliclipopeptideantibioticpelgipeptinproducedbyitpaenibacilluselgiiit AT zhoushuanglin identificationandfunctionalanalysisofgeneclusterinvolvementinbiosynthesisofthecycliclipopeptideantibioticpelgipeptinproducedbyitpaenibacilluselgiiit AT dingrui identificationandfunctionalanalysisofgeneclusterinvolvementinbiosynthesisofthecycliclipopeptideantibioticpelgipeptinproducedbyitpaenibacilluselgiiit AT zhaowenpeng identificationandfunctionalanalysisofgeneclusterinvolvementinbiosynthesisofthecycliclipopeptideantibioticpelgipeptinproducedbyitpaenibacilluselgiiit AT liou identificationandfunctionalanalysisofgeneclusterinvolvementinbiosynthesisofthecycliclipopeptideantibioticpelgipeptinproducedbyitpaenibacilluselgiiit AT wuxuechang identificationandfunctionalanalysisofgeneclusterinvolvementinbiosynthesisofthecycliclipopeptideantibioticpelgipeptinproducedbyitpaenibacilluselgiiit |