Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes
Plant-parasitic and entomopathogenic nematodes (PPNs and EPNs) are key groups in crop production systems. This study aims at optimizing nematode sampling and extraction methods to benefit integrated pest management (IPM) through (a) management of PPNs and (b) use of EPNs. The impacts of these method...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-03-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/4/629 |
| _version_ | 1797539933255106560 |
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| author | Mahfouz M. M. Abd-Elgawad |
| author_facet | Mahfouz M. M. Abd-Elgawad |
| author_sort | Mahfouz M. M. Abd-Elgawad |
| collection | DOAJ |
| description | Plant-parasitic and entomopathogenic nematodes (PPNs and EPNs) are key groups in crop production systems. This study aims at optimizing nematode sampling and extraction methods to benefit integrated pest management (IPM) through (a) management of PPNs and (b) use of EPNs. The impacts of these methods on PPNs and EPNs to achieve cost-effective and efficient IPM programs are presented. The common misuses of sampling and extraction methods are discussed. Professionals engaged in IPM should consider sampling the reliability level in the light of the intended goal, location, crop value, susceptibility, nematode species, and available funds. Logical sampling methodology should be expanded to integrate various factors that can recover extra EPN isolates with differential pathogenicity. It should seek for the best EPN-host matching. Merits of repeated baiting for EPN extraction from soil and sieving for PPN recovery from suspensions are presented. Their extraction values may be modelled to quantify the efficiency of nematode separation. The use of proper indices of dispersion to enhance the biocontrol potential of EPNs or save costs in nematicidal applications is ideally compatible with IPM programs. Selecting an extraction method may sometimes require further tests to find the best extraction method of the existing fauna and/or flora. Cons and pros of modern sampling and extraction techniques are highlighted. |
| first_indexed | 2024-03-10T12:52:51Z |
| format | Article |
| id | doaj.art-06a608d9a70441bab5a71fe91f15b089 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T12:52:51Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-06a608d9a70441bab5a71fe91f15b0892023-11-21T12:05:56ZengMDPI AGPlants2223-77472021-03-0110462910.3390/plants10040629Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic NematodesMahfouz M. M. Abd-Elgawad0National Research Center, Plant Pathology Department, Agricultural and Biological Research Division, El-behooth St., Dokki 12622, EgyptPlant-parasitic and entomopathogenic nematodes (PPNs and EPNs) are key groups in crop production systems. This study aims at optimizing nematode sampling and extraction methods to benefit integrated pest management (IPM) through (a) management of PPNs and (b) use of EPNs. The impacts of these methods on PPNs and EPNs to achieve cost-effective and efficient IPM programs are presented. The common misuses of sampling and extraction methods are discussed. Professionals engaged in IPM should consider sampling the reliability level in the light of the intended goal, location, crop value, susceptibility, nematode species, and available funds. Logical sampling methodology should be expanded to integrate various factors that can recover extra EPN isolates with differential pathogenicity. It should seek for the best EPN-host matching. Merits of repeated baiting for EPN extraction from soil and sieving for PPN recovery from suspensions are presented. Their extraction values may be modelled to quantify the efficiency of nematode separation. The use of proper indices of dispersion to enhance the biocontrol potential of EPNs or save costs in nematicidal applications is ideally compatible with IPM programs. Selecting an extraction method may sometimes require further tests to find the best extraction method of the existing fauna and/or flora. Cons and pros of modern sampling and extraction techniques are highlighted.https://www.mdpi.com/2223-7747/10/4/629index of dispersionIPMmodellingmolecular approachessampling and extraction |
| spellingShingle | Mahfouz M. M. Abd-Elgawad Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes Plants index of dispersion IPM modelling molecular approaches sampling and extraction |
| title | Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes |
| title_full | Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes |
| title_fullStr | Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes |
| title_full_unstemmed | Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes |
| title_short | Optimizing Sampling and Extraction Methods for Plant-Parasitic and Entomopathogenic Nematodes |
| title_sort | optimizing sampling and extraction methods for plant parasitic and entomopathogenic nematodes |
| topic | index of dispersion IPM modelling molecular approaches sampling and extraction |
| url | https://www.mdpi.com/2223-7747/10/4/629 |
| work_keys_str_mv | AT mahfouzmmabdelgawad optimizingsamplingandextractionmethodsforplantparasiticandentomopathogenicnematodes |