Antibacterial Effect of Acoustic Cavitation Promoted by Mesoporous Silicon Nanoparticles
As-prepared mesoporous silicon nanoparticles, which were synthesized by electrochemical etching of crystalline silicon wafers followed by high-energy milling in water, were explored as a sonosensitizer in aqueous media under irradiation with low-intensity ultrasound at 0.88 MHz. Due to the mixed oxi...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/2/1065 |
| _version_ | 1797441593536413696 |
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| author | Andrey Sviridov Svetlana Mazina Anna Ostapenko Alexander Nikolaev Victor Timoshenko |
| author_facet | Andrey Sviridov Svetlana Mazina Anna Ostapenko Alexander Nikolaev Victor Timoshenko |
| author_sort | Andrey Sviridov |
| collection | DOAJ |
| description | As-prepared mesoporous silicon nanoparticles, which were synthesized by electrochemical etching of crystalline silicon wafers followed by high-energy milling in water, were explored as a sonosensitizer in aqueous media under irradiation with low-intensity ultrasound at 0.88 MHz. Due to the mixed oxide-hydride coating of the nanoparticles’ surfaces, they showed both acceptable colloidal stability and sonosensitization of the acoustic cavitation. The latter was directly measured and quantified as a cavitation energy index, i.e., time integral of the magnitude of ultrasound subharmonics. The index turned out to be several times greater for nanoparticle suspensions as compared to pure water, and it depended nonmonotonically on nanoparticle concentration. In vitro tests with <i>Lactobacillus casei</i> revealed a dramatic drop of the bacterial viability and damage of the cells after ultrasonic irradiation with intensity of about 1 W/cm<sup>2</sup> in the presence of nanoparticles, which themselves are almost non-toxic at the studied concentrations of about 1 mg/mL. The experimental results prove that nanoparticle-sensitized cavitation bubbles nearby bacteria can cause bacterial lysis and death. The sonosensitizing properties of freshly prepared mesoporous silicon nanoparticles are beneficial for their application in mild antibacterial therapy and treatment of liquid media. |
| first_indexed | 2024-03-09T12:25:24Z |
| format | Article |
| id | doaj.art-06b1fd03b0c74c9aaac2fa94d5c8b7f4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T12:25:24Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-06b1fd03b0c74c9aaac2fa94d5c8b7f42023-11-30T22:34:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01242106510.3390/ijms24021065Antibacterial Effect of Acoustic Cavitation Promoted by Mesoporous Silicon NanoparticlesAndrey Sviridov0Svetlana Mazina1Anna Ostapenko2Alexander Nikolaev3Victor Timoshenko4Faculty of Physics, Lomonosov Moscow State University, Leninskie Gory 1-2, 119991 Moscow, RussiaResearch and Technical Centre of Radiation-Chemical Safety and Hygiene, FMBA, Schukinskaya St 40, 123182 Moscow, RussiaFaculty of Physics, Lomonosov Moscow State University, Leninskie Gory 1-2, 119991 Moscow, RussiaFaculty of Chemistry, Lomonosov Moscow State University, Leninskie Gory 1-3, 119991 Moscow, RussiaFaculty of Physics, Lomonosov Moscow State University, Leninskie Gory 1-2, 119991 Moscow, RussiaAs-prepared mesoporous silicon nanoparticles, which were synthesized by electrochemical etching of crystalline silicon wafers followed by high-energy milling in water, were explored as a sonosensitizer in aqueous media under irradiation with low-intensity ultrasound at 0.88 MHz. Due to the mixed oxide-hydride coating of the nanoparticles’ surfaces, they showed both acceptable colloidal stability and sonosensitization of the acoustic cavitation. The latter was directly measured and quantified as a cavitation energy index, i.e., time integral of the magnitude of ultrasound subharmonics. The index turned out to be several times greater for nanoparticle suspensions as compared to pure water, and it depended nonmonotonically on nanoparticle concentration. In vitro tests with <i>Lactobacillus casei</i> revealed a dramatic drop of the bacterial viability and damage of the cells after ultrasonic irradiation with intensity of about 1 W/cm<sup>2</sup> in the presence of nanoparticles, which themselves are almost non-toxic at the studied concentrations of about 1 mg/mL. The experimental results prove that nanoparticle-sensitized cavitation bubbles nearby bacteria can cause bacterial lysis and death. The sonosensitizing properties of freshly prepared mesoporous silicon nanoparticles are beneficial for their application in mild antibacterial therapy and treatment of liquid media.https://www.mdpi.com/1422-0067/24/2/1065porous siliconsilicon nanoparticlesacoustic cavitationlow-intensity ultrasoundsubharmonicsantibacterial effect |
| spellingShingle | Andrey Sviridov Svetlana Mazina Anna Ostapenko Alexander Nikolaev Victor Timoshenko Antibacterial Effect of Acoustic Cavitation Promoted by Mesoporous Silicon Nanoparticles International Journal of Molecular Sciences porous silicon silicon nanoparticles acoustic cavitation low-intensity ultrasound subharmonics antibacterial effect |
| title | Antibacterial Effect of Acoustic Cavitation Promoted by Mesoporous Silicon Nanoparticles |
| title_full | Antibacterial Effect of Acoustic Cavitation Promoted by Mesoporous Silicon Nanoparticles |
| title_fullStr | Antibacterial Effect of Acoustic Cavitation Promoted by Mesoporous Silicon Nanoparticles |
| title_full_unstemmed | Antibacterial Effect of Acoustic Cavitation Promoted by Mesoporous Silicon Nanoparticles |
| title_short | Antibacterial Effect of Acoustic Cavitation Promoted by Mesoporous Silicon Nanoparticles |
| title_sort | antibacterial effect of acoustic cavitation promoted by mesoporous silicon nanoparticles |
| topic | porous silicon silicon nanoparticles acoustic cavitation low-intensity ultrasound subharmonics antibacterial effect |
| url | https://www.mdpi.com/1422-0067/24/2/1065 |
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