Nrf2-Activating Bioactive Peptides Exert Anti-Inflammatory Activity through Inhibition of the NF-κB Pathway
Redox status and inflammation are related to the pathogenesis of the majority of diseases. Therefore, understanding the role of specific food-derived molecules in the regulation of their specific pathways is a relevant issue. Our previous studies indicated that <b>K-8-K</b> and <b>...
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| Format: | Article |
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/8/4382 |
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| author | Federica Tonolo Alessandra Folda Valeria Scalcon Oriano Marin Alberto Bindoli Maria Pia Rigobello |
| author_facet | Federica Tonolo Alessandra Folda Valeria Scalcon Oriano Marin Alberto Bindoli Maria Pia Rigobello |
| author_sort | Federica Tonolo |
| collection | DOAJ |
| description | Redox status and inflammation are related to the pathogenesis of the majority of diseases. Therefore, understanding the role of specific food-derived molecules in the regulation of their specific pathways is a relevant issue. Our previous studies indicated that <b>K-8-K</b> and <b>S-10-S</b>, milk and soy-derived bioactive peptides, respectively, exert antioxidant effects through activation of the Keap1/Nrf2 pathway. A crosstalk between Nrf2 and NF-κB, mediated by the action of heme oxygenase (HO-1), is well known. On this basis, we studied if these peptides, in addition to their antioxidant activity, could exert anti-inflammatory effects in human cells. First, we observed an increase of HO-1 expression in Caco-2 cells treated with <b>K-8-K</b> and <b>S-10-S</b>, following the activation of the Keap1/Nrf2 pathway. Moreover, when cells are treated with the two peptides and stimulated by TNF-α, the levels of NF-κB in the nucleus decreased in comparison with TNF-α alone. In the same conditions, we observed the downregulation of the gene expression of proinflammatory cytokines (<i>IL1B</i>, <i>IL6</i>, and <i>TNF</i>), while the anti-inflammatory cytokine gene, <i>IL1RN</i>, was upregulated in Caco-2 cells processed as reported above. Then, when the cells were pretreated with the two peptides and stimulated with LPS, a different proinflammatory factor, (TNF-α) was estimated to have a lower secretion in the supernatant of cells. In conclusion, these observations confirmed that Nrf2-activating bioactive peptides, <b>K-8-K</b> and <b>S-10-S</b>, exerted anti-inflammatory effects by inhibiting the NF-κB pathway. |
| first_indexed | 2024-03-09T10:33:41Z |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:33:41Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-06b2d6a88c444fad83be070bfd7ecf382023-12-01T21:04:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01238438210.3390/ijms23084382Nrf2-Activating Bioactive Peptides Exert Anti-Inflammatory Activity through Inhibition of the NF-κB PathwayFederica Tonolo0Alessandra Folda1Valeria Scalcon2Oriano Marin3Alberto Bindoli4Maria Pia Rigobello5Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/b, 35131 Padova, ItalyDepartment of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/b, 35131 Padova, ItalyDepartment of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/b, 35131 Padova, ItalyDepartment of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/b, 35131 Padova, ItalyInstitute of Neuroscience, CNR, Via G Colombo 3, 35131 Padova, ItalyDepartment of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/b, 35131 Padova, ItalyRedox status and inflammation are related to the pathogenesis of the majority of diseases. Therefore, understanding the role of specific food-derived molecules in the regulation of their specific pathways is a relevant issue. Our previous studies indicated that <b>K-8-K</b> and <b>S-10-S</b>, milk and soy-derived bioactive peptides, respectively, exert antioxidant effects through activation of the Keap1/Nrf2 pathway. A crosstalk between Nrf2 and NF-κB, mediated by the action of heme oxygenase (HO-1), is well known. On this basis, we studied if these peptides, in addition to their antioxidant activity, could exert anti-inflammatory effects in human cells. First, we observed an increase of HO-1 expression in Caco-2 cells treated with <b>K-8-K</b> and <b>S-10-S</b>, following the activation of the Keap1/Nrf2 pathway. Moreover, when cells are treated with the two peptides and stimulated by TNF-α, the levels of NF-κB in the nucleus decreased in comparison with TNF-α alone. In the same conditions, we observed the downregulation of the gene expression of proinflammatory cytokines (<i>IL1B</i>, <i>IL6</i>, and <i>TNF</i>), while the anti-inflammatory cytokine gene, <i>IL1RN</i>, was upregulated in Caco-2 cells processed as reported above. Then, when the cells were pretreated with the two peptides and stimulated with LPS, a different proinflammatory factor, (TNF-α) was estimated to have a lower secretion in the supernatant of cells. In conclusion, these observations confirmed that Nrf2-activating bioactive peptides, <b>K-8-K</b> and <b>S-10-S</b>, exerted anti-inflammatory effects by inhibiting the NF-κB pathway.https://www.mdpi.com/1422-0067/23/8/4382bioactive peptidesantioxidantanti-inflammatoryNrf2NF-κB |
| spellingShingle | Federica Tonolo Alessandra Folda Valeria Scalcon Oriano Marin Alberto Bindoli Maria Pia Rigobello Nrf2-Activating Bioactive Peptides Exert Anti-Inflammatory Activity through Inhibition of the NF-κB Pathway International Journal of Molecular Sciences bioactive peptides antioxidant anti-inflammatory Nrf2 NF-κB |
| title | Nrf2-Activating Bioactive Peptides Exert Anti-Inflammatory Activity through Inhibition of the NF-κB Pathway |
| title_full | Nrf2-Activating Bioactive Peptides Exert Anti-Inflammatory Activity through Inhibition of the NF-κB Pathway |
| title_fullStr | Nrf2-Activating Bioactive Peptides Exert Anti-Inflammatory Activity through Inhibition of the NF-κB Pathway |
| title_full_unstemmed | Nrf2-Activating Bioactive Peptides Exert Anti-Inflammatory Activity through Inhibition of the NF-κB Pathway |
| title_short | Nrf2-Activating Bioactive Peptides Exert Anti-Inflammatory Activity through Inhibition of the NF-κB Pathway |
| title_sort | nrf2 activating bioactive peptides exert anti inflammatory activity through inhibition of the nf κb pathway |
| topic | bioactive peptides antioxidant anti-inflammatory Nrf2 NF-κB |
| url | https://www.mdpi.com/1422-0067/23/8/4382 |
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