Spectrophotometric Enzyme Assays for High-Throughput Screening
This paper reviews high-throughput screening enzyme assays developed in our laboratory over the last ten years. These enzyme assays were initially developed for the purpose of discovering catalytic antibodies by screening cell culture supernatants, but have proved generally useful for testing enzyme...
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| Format: | Article |
| Language: | English |
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University of Zagreb Faculty of Food Technology and Biotechnology
2004-01-01
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| Series: | Food Technology and Biotechnology |
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| Online Access: | http://hrcak.srce.hr/file/163385 |
| _version_ | 1797421438226923520 |
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| author | Jean-Louis Reymond |
| author_facet | Jean-Louis Reymond |
| author_sort | Jean-Louis Reymond |
| collection | DOAJ |
| description | This paper reviews high-throughput screening enzyme assays developed in our laboratory over the last ten years. These enzyme assays were initially developed for the purpose of discovering catalytic antibodies by screening cell culture supernatants, but have proved generally useful for testing enzyme activities. Examples include TLC-based screening using acridone-labeled substrates, fluorogenic assays based on the β-elimination of umbelliferone or nitrophenol, and indirect assays such as the back-titration method with adrenaline and the copper-calcein fluorescence assay for aminoacids. |
| first_indexed | 2024-03-09T07:17:20Z |
| format | Article |
| id | doaj.art-06b61bbb456b4374b99760c7daaef52b |
| institution | Directory Open Access Journal |
| issn | 1330-9862 1334-2606 |
| language | English |
| last_indexed | 2024-03-09T07:17:20Z |
| publishDate | 2004-01-01 |
| publisher | University of Zagreb Faculty of Food Technology and Biotechnology |
| record_format | Article |
| series | Food Technology and Biotechnology |
| spelling | doaj.art-06b61bbb456b4374b99760c7daaef52b2023-12-03T08:15:03ZengUniversity of Zagreb Faculty of Food Technology and BiotechnologyFood Technology and Biotechnology1330-98621334-26062004-01-01424265269Spectrophotometric Enzyme Assays for High-Throughput ScreeningJean-Louis Reymond0Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, SwitzerlandThis paper reviews high-throughput screening enzyme assays developed in our laboratory over the last ten years. These enzyme assays were initially developed for the purpose of discovering catalytic antibodies by screening cell culture supernatants, but have proved generally useful for testing enzyme activities. Examples include TLC-based screening using acridone-labeled substrates, fluorogenic assays based on the β-elimination of umbelliferone or nitrophenol, and indirect assays such as the back-titration method with adrenaline and the copper-calcein fluorescence assay for aminoacids.http://hrcak.srce.hr/file/163385screening of enzyme activitiesTLC-based screeningfluorogenic assayadrenalin test for enzymesadrenalin test and copper-calcein assay for amino acids |
| spellingShingle | Jean-Louis Reymond Spectrophotometric Enzyme Assays for High-Throughput Screening Food Technology and Biotechnology screening of enzyme activities TLC-based screening fluorogenic assay adrenalin test for enzymes adrenalin test and copper-calcein assay for amino acids |
| title | Spectrophotometric Enzyme Assays for High-Throughput Screening |
| title_full | Spectrophotometric Enzyme Assays for High-Throughput Screening |
| title_fullStr | Spectrophotometric Enzyme Assays for High-Throughput Screening |
| title_full_unstemmed | Spectrophotometric Enzyme Assays for High-Throughput Screening |
| title_short | Spectrophotometric Enzyme Assays for High-Throughput Screening |
| title_sort | spectrophotometric enzyme assays for high throughput screening |
| topic | screening of enzyme activities TLC-based screening fluorogenic assay adrenalin test for enzymes adrenalin test and copper-calcein assay for amino acids |
| url | http://hrcak.srce.hr/file/163385 |
| work_keys_str_mv | AT jeanlouisreymond spectrophotometricenzymeassaysforhighthroughputscreening |