Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection
Lateral flow assays (lateral flow immunoassays and nucleic acid lateral flow assays) have experienced a great boom in a wide variety of early diagnostic and screening applications. As opposed to conventional examinations (High Performance Liquid Chromatography, Polymerase Chain Reaction, Gas chromat...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-02-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/4/1058 |
| _version_ | 1827588981442740224 |
|---|---|
| author | Zidane Qriouet Yahia Cherrah Hassan Sefrioui Zineb Qmichou |
| author_facet | Zidane Qriouet Yahia Cherrah Hassan Sefrioui Zineb Qmichou |
| author_sort | Zidane Qriouet |
| collection | DOAJ |
| description | Lateral flow assays (lateral flow immunoassays and nucleic acid lateral flow assays) have experienced a great boom in a wide variety of early diagnostic and screening applications. As opposed to conventional examinations (High Performance Liquid Chromatography, Polymerase Chain Reaction, Gas chromatography-Mass Spectrometry, etc.), they obtain the results of a sample’s analysis within a short period. In resource-limited areas, these tests must be simple, reliable, and inexpensive. In this review, we outline the production process of antibodies against drugs of abuse (such as heroin, amphetamine, benzodiazepines, cannabis, etc.), used in lateral flow immunoassays as revelation or detection molecules, with a focus on the components, the principles, the formats, and the mechanisms of reaction of these assays. Further, we report the monoclonal antibody advantages over the polyclonal ones used against drugs of abuse. The perspective on aptamer use for lateral flow assay development was also discussed as a possible alternative to antibodies in view of improving the limit of detection, sensitivity, and specificity of lateral flow assays. |
| first_indexed | 2024-03-09T00:47:14Z |
| format | Article |
| id | doaj.art-06bc4ee2b0f7481896e9e69981987b79 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T00:47:14Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-06bc4ee2b0f7481896e9e69981987b792023-12-11T17:27:06ZengMDPI AGMolecules1420-30492021-02-01264105810.3390/molecules26041058Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse DetectionZidane Qriouet0Yahia Cherrah1Hassan Sefrioui2Zineb Qmichou3Medical Biotechnology Center, Moroccan Foundation for Advanced Science, Innovation & Research (MAScIR), Rabat 10100, MoroccoLaboratoire de Pharmacologie et Toxicologie, Faculté de Médecine et de Pharmacie, Université Mohammed V-Souissi, Rabat 10100, MoroccoMedical Biotechnology Center, Moroccan Foundation for Advanced Science, Innovation & Research (MAScIR), Rabat 10100, MoroccoMedical Biotechnology Center, Moroccan Foundation for Advanced Science, Innovation & Research (MAScIR), Rabat 10100, MoroccoLateral flow assays (lateral flow immunoassays and nucleic acid lateral flow assays) have experienced a great boom in a wide variety of early diagnostic and screening applications. As opposed to conventional examinations (High Performance Liquid Chromatography, Polymerase Chain Reaction, Gas chromatography-Mass Spectrometry, etc.), they obtain the results of a sample’s analysis within a short period. In resource-limited areas, these tests must be simple, reliable, and inexpensive. In this review, we outline the production process of antibodies against drugs of abuse (such as heroin, amphetamine, benzodiazepines, cannabis, etc.), used in lateral flow immunoassays as revelation or detection molecules, with a focus on the components, the principles, the formats, and the mechanisms of reaction of these assays. Further, we report the monoclonal antibody advantages over the polyclonal ones used against drugs of abuse. The perspective on aptamer use for lateral flow assay development was also discussed as a possible alternative to antibodies in view of improving the limit of detection, sensitivity, and specificity of lateral flow assays.https://www.mdpi.com/1420-3049/26/4/1058drugs of abuselateral flow assay (LFA), immunochromatographymonoclonal antibodies and detection |
| spellingShingle | Zidane Qriouet Yahia Cherrah Hassan Sefrioui Zineb Qmichou Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection Molecules drugs of abuse lateral flow assay (LFA), immunochromatography monoclonal antibodies and detection |
| title | Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection |
| title_full | Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection |
| title_fullStr | Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection |
| title_full_unstemmed | Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection |
| title_short | Monoclonal Antibodies Application in Lateral Flow Immunochromatographic Assays for Drugs of Abuse Detection |
| title_sort | monoclonal antibodies application in lateral flow immunochromatographic assays for drugs of abuse detection |
| topic | drugs of abuse lateral flow assay (LFA), immunochromatography monoclonal antibodies and detection |
| url | https://www.mdpi.com/1420-3049/26/4/1058 |
| work_keys_str_mv | AT zidaneqriouet monoclonalantibodiesapplicationinlateralflowimmunochromatographicassaysfordrugsofabusedetection AT yahiacherrah monoclonalantibodiesapplicationinlateralflowimmunochromatographicassaysfordrugsofabusedetection AT hassansefrioui monoclonalantibodiesapplicationinlateralflowimmunochromatographicassaysfordrugsofabusedetection AT zinebqmichou monoclonalantibodiesapplicationinlateralflowimmunochromatographicassaysfordrugsofabusedetection |