Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility Study
The development of biomedical soft- or hardware frequently includes testing in animals. However, large efforts have been made to reduce the number of animal experiments, according to the 3Rs principle. Simultaneously, a significant number of surplus animals are euthanized without scientific necessit...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/22/3532 |
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| author | Matthias Manfred Deininger Carl-Friedrich Benner Lasse Johannes Strudthoff Steffen Leonhardt Christian Simon Bruells Gernot Marx Christian Bleilevens Thomas Breuer |
| author_facet | Matthias Manfred Deininger Carl-Friedrich Benner Lasse Johannes Strudthoff Steffen Leonhardt Christian Simon Bruells Gernot Marx Christian Bleilevens Thomas Breuer |
| author_sort | Matthias Manfred Deininger |
| collection | DOAJ |
| description | The development of biomedical soft- or hardware frequently includes testing in animals. However, large efforts have been made to reduce the number of animal experiments, according to the 3Rs principle. Simultaneously, a significant number of surplus animals are euthanized without scientific necessity. The primary aim of this study was to establish a post-mortem rat perfusion model using extracorporeal membrane oxygenation (ECMO) in surplus rat cadavers and generate first post vivo results concerning the oxygenation performance of a recently developed ECMO membrane oxygenator. Four rats were euthanized and connected post-mortem to a venous–arterial ECMO circulation for up to eight hours. Angiographic perfusion proofs, blood gas analyses and blood oxygenation calculations were performed. The mean preparation time for the ECMO system was 791 ± 29 s and sufficient organ perfusion could be maintained for 463 ± 26 min, proofed via angiographic imaging and a mean femoral arterial pressure of 43 ± 17 mmHg. A stable partial oxygen pressure, a 73% rise in arterial oxygen concentration and an exponentially increasing oxygen extraction ratio up to 4.75 times were shown. Considering the 3Rs, the established post-mortal ECMO perfusion rat model using surplus animals represents a promising alternative to models using live animals. Given the preserved organ perfusion, its use could be conceivable for various biomedical device testing. |
| first_indexed | 2024-03-09T17:05:06Z |
| format | Article |
| id | doaj.art-06bd2940be724749b2d9abb7ebdc92b6 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T17:05:06Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-06bd2940be724749b2d9abb7ebdc92b62023-11-24T14:24:53ZengMDPI AGAnimals2076-26152023-11-011322353210.3390/ani13223532Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility StudyMatthias Manfred Deininger0Carl-Friedrich Benner1Lasse Johannes Strudthoff2Steffen Leonhardt3Christian Simon Bruells4Gernot Marx5Christian Bleilevens6Thomas Breuer7Department of Intensive and Intermediate Care, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyMedical Information Technology, Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Cardiovascular Engineering, Institute of Applied Medical Engineering, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyMedical Information Technology, Helmholtz-Institute for Biomedical Engineering, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Anesthesia, Intensive and Emergency Medicine, Marien Kliniken, 57072 Siegen, GermanyDepartment of Intensive and Intermediate Care, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Anesthesiology, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Intensive and Intermediate Care, Medical Faculty, RWTH Aachen University, 52074 Aachen, GermanyThe development of biomedical soft- or hardware frequently includes testing in animals. However, large efforts have been made to reduce the number of animal experiments, according to the 3Rs principle. Simultaneously, a significant number of surplus animals are euthanized without scientific necessity. The primary aim of this study was to establish a post-mortem rat perfusion model using extracorporeal membrane oxygenation (ECMO) in surplus rat cadavers and generate first post vivo results concerning the oxygenation performance of a recently developed ECMO membrane oxygenator. Four rats were euthanized and connected post-mortem to a venous–arterial ECMO circulation for up to eight hours. Angiographic perfusion proofs, blood gas analyses and blood oxygenation calculations were performed. The mean preparation time for the ECMO system was 791 ± 29 s and sufficient organ perfusion could be maintained for 463 ± 26 min, proofed via angiographic imaging and a mean femoral arterial pressure of 43 ± 17 mmHg. A stable partial oxygen pressure, a 73% rise in arterial oxygen concentration and an exponentially increasing oxygen extraction ratio up to 4.75 times were shown. Considering the 3Rs, the established post-mortal ECMO perfusion rat model using surplus animals represents a promising alternative to models using live animals. Given the preserved organ perfusion, its use could be conceivable for various biomedical device testing.https://www.mdpi.com/2076-2615/13/22/3532ECMOperfusionpost-mortemoxygenatorrat modelSeldinger technique |
| spellingShingle | Matthias Manfred Deininger Carl-Friedrich Benner Lasse Johannes Strudthoff Steffen Leonhardt Christian Simon Bruells Gernot Marx Christian Bleilevens Thomas Breuer Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility Study Animals ECMO perfusion post-mortem oxygenator rat model Seldinger technique |
| title | Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility Study |
| title_full | Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility Study |
| title_fullStr | Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility Study |
| title_full_unstemmed | Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility Study |
| title_short | Post-Mortem Extracorporeal Membrane Oxygenation Perfusion Rat Model: A Feasibility Study |
| title_sort | post mortem extracorporeal membrane oxygenation perfusion rat model a feasibility study |
| topic | ECMO perfusion post-mortem oxygenator rat model Seldinger technique |
| url | https://www.mdpi.com/2076-2615/13/22/3532 |
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