Urethral dysfunction in female mice with estrogen receptor β deficiency.
Estrogen has various regulatory functions in the growth, development, and differentiation of the female urogenital system. This study investigated the roles of ERβ in stress urinary incontinence (SUI). Wild-type (ERβ(+/+)) and knockout (ERβ(-/-)) female mice were generated (aged 6-8 weeks, n = 6) an...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4183540?pdf=render |
| _version_ | 1819231754192420864 |
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| author | Yung-Hsiang Chen Chao-Jung Chen Shuyuan Yeh Yu-Ning Lin Yang-Chang Wu Wen-Tsong Hsieh Bor-Tsang Wu Wen-Lung Ma Wen-Chi Chen Chawnshang Chang Huey-Yi Chen |
| author_facet | Yung-Hsiang Chen Chao-Jung Chen Shuyuan Yeh Yu-Ning Lin Yang-Chang Wu Wen-Tsong Hsieh Bor-Tsang Wu Wen-Lung Ma Wen-Chi Chen Chawnshang Chang Huey-Yi Chen |
| author_sort | Yung-Hsiang Chen |
| collection | DOAJ |
| description | Estrogen has various regulatory functions in the growth, development, and differentiation of the female urogenital system. This study investigated the roles of ERβ in stress urinary incontinence (SUI). Wild-type (ERβ(+/+)) and knockout (ERβ(-/-)) female mice were generated (aged 6-8 weeks, n = 6) and urethral function and protein expression were measured. Leak point pressures (LPP) and maximum urethral closure pressure (MUCP) were assessed in mice under urethane anesthesia. After the measurements, the urethras were removed for proteomic analysis using label-free quantitative proteomics by nano-liquid chromatography-mass spectrometry (LC-MS/MS) analysis. The interaction between these proteins was further analysed using MetaCore. Lastly, Western blot was used to confirm the candidate proteins. Compared with the ERβ(+/+) group, the LPP and MUCP values of the ERβ(-/-) group were significantly decreased. Additionally, we identified 85 differentially expressed proteins in the urethra of ERβ(-/-) female mice; 57 proteins were up-regulated and 28 were down-regulated. The majority of the ERβ knockout-modified proteins were involved in cell-matrix adhesion, metabolism, immune response, signal transduction, nuclear receptor translational regelation, and muscle contraction and development. Western blot confirmed the up-regulation of myosin and collagen in urethra. By contrast, elastin was down-regulated in the ERβ(-/-) mice. This study is the first study to estimate protein expression changes in urethras from ERβ(-/-) female mice. These changes could be related to the molecular mechanism of ERβ in SUI. |
| first_indexed | 2024-12-23T11:49:59Z |
| format | Article |
| id | doaj.art-06bd903427dd4518be8780fff784c879 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-23T11:49:59Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-06bd903427dd4518be8780fff784c8792022-12-21T17:48:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10905810.1371/journal.pone.0109058Urethral dysfunction in female mice with estrogen receptor β deficiency.Yung-Hsiang ChenChao-Jung ChenShuyuan YehYu-Ning LinYang-Chang WuWen-Tsong HsiehBor-Tsang WuWen-Lung MaWen-Chi ChenChawnshang ChangHuey-Yi ChenEstrogen has various regulatory functions in the growth, development, and differentiation of the female urogenital system. This study investigated the roles of ERβ in stress urinary incontinence (SUI). Wild-type (ERβ(+/+)) and knockout (ERβ(-/-)) female mice were generated (aged 6-8 weeks, n = 6) and urethral function and protein expression were measured. Leak point pressures (LPP) and maximum urethral closure pressure (MUCP) were assessed in mice under urethane anesthesia. After the measurements, the urethras were removed for proteomic analysis using label-free quantitative proteomics by nano-liquid chromatography-mass spectrometry (LC-MS/MS) analysis. The interaction between these proteins was further analysed using MetaCore. Lastly, Western blot was used to confirm the candidate proteins. Compared with the ERβ(+/+) group, the LPP and MUCP values of the ERβ(-/-) group were significantly decreased. Additionally, we identified 85 differentially expressed proteins in the urethra of ERβ(-/-) female mice; 57 proteins were up-regulated and 28 were down-regulated. The majority of the ERβ knockout-modified proteins were involved in cell-matrix adhesion, metabolism, immune response, signal transduction, nuclear receptor translational regelation, and muscle contraction and development. Western blot confirmed the up-regulation of myosin and collagen in urethra. By contrast, elastin was down-regulated in the ERβ(-/-) mice. This study is the first study to estimate protein expression changes in urethras from ERβ(-/-) female mice. These changes could be related to the molecular mechanism of ERβ in SUI.http://europepmc.org/articles/PMC4183540?pdf=render |
| spellingShingle | Yung-Hsiang Chen Chao-Jung Chen Shuyuan Yeh Yu-Ning Lin Yang-Chang Wu Wen-Tsong Hsieh Bor-Tsang Wu Wen-Lung Ma Wen-Chi Chen Chawnshang Chang Huey-Yi Chen Urethral dysfunction in female mice with estrogen receptor β deficiency. PLoS ONE |
| title | Urethral dysfunction in female mice with estrogen receptor β deficiency. |
| title_full | Urethral dysfunction in female mice with estrogen receptor β deficiency. |
| title_fullStr | Urethral dysfunction in female mice with estrogen receptor β deficiency. |
| title_full_unstemmed | Urethral dysfunction in female mice with estrogen receptor β deficiency. |
| title_short | Urethral dysfunction in female mice with estrogen receptor β deficiency. |
| title_sort | urethral dysfunction in female mice with estrogen receptor β deficiency |
| url | http://europepmc.org/articles/PMC4183540?pdf=render |
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