Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy.
In this work, we investigated the effect of pH on Streptomyces M37 growth and its acarbose biosynthesis ability. We observed that low pH was beneficial for cell growth, whereas high pH favored acarbose synthesis. Moreover, addition of glucose and maltose to the fermentation medium after 72 h of cult...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5325187?pdf=render |
| _version_ | 1828476406638575616 |
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| author | Fei Ren Long Chen Shuangli Xiong Qunyi Tong |
| author_facet | Fei Ren Long Chen Shuangli Xiong Qunyi Tong |
| author_sort | Fei Ren |
| collection | DOAJ |
| description | In this work, we investigated the effect of pH on Streptomyces M37 growth and its acarbose biosynthesis ability. We observed that low pH was beneficial for cell growth, whereas high pH favored acarbose synthesis. Moreover, addition of glucose and maltose to the fermentation medium after 72 h of cultivation promoted acarbose production. Based on these results, a two-stage fermentation strategy was developed to improve acarbose production. Accordingly, pH was kept at 7.0 during the first 72 h and switched to 8.0 after that. At the same time, glucose and maltose were fed to increase acarbose accumulation. With this strategy, we achieved an acarbose titer of 6210 mg/L, representing an 85.7% increase over traditional batch fermentation without pH control. Finally, we determined that the increased acarbose production was related to the high activity of glutamate dehydrogenase and glucose 6-phosphate dehydrogenase. |
| first_indexed | 2024-12-11T06:28:19Z |
| format | Article |
| id | doaj.art-06be2715b2a34cd0ba27ac98303e70a8 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T06:28:19Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-06be2715b2a34cd0ba27ac98303e70a82022-12-22T01:17:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01122e016698510.1371/journal.pone.0166985Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy.Fei RenLong ChenShuangli XiongQunyi TongIn this work, we investigated the effect of pH on Streptomyces M37 growth and its acarbose biosynthesis ability. We observed that low pH was beneficial for cell growth, whereas high pH favored acarbose synthesis. Moreover, addition of glucose and maltose to the fermentation medium after 72 h of cultivation promoted acarbose production. Based on these results, a two-stage fermentation strategy was developed to improve acarbose production. Accordingly, pH was kept at 7.0 during the first 72 h and switched to 8.0 after that. At the same time, glucose and maltose were fed to increase acarbose accumulation. With this strategy, we achieved an acarbose titer of 6210 mg/L, representing an 85.7% increase over traditional batch fermentation without pH control. Finally, we determined that the increased acarbose production was related to the high activity of glutamate dehydrogenase and glucose 6-phosphate dehydrogenase.http://europepmc.org/articles/PMC5325187?pdf=render |
| spellingShingle | Fei Ren Long Chen Shuangli Xiong Qunyi Tong Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy. PLoS ONE |
| title | Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy. |
| title_full | Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy. |
| title_fullStr | Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy. |
| title_full_unstemmed | Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy. |
| title_short | Enhanced acarbose production by Streptomyces M37 using a two-stage fermentation strategy. |
| title_sort | enhanced acarbose production by streptomyces m37 using a two stage fermentation strategy |
| url | http://europepmc.org/articles/PMC5325187?pdf=render |
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