Immunoglobulin genes and T-cell receptors as molecular markers in children with acute lymphoblastic leukaemia
Introduction. Acute lymphoblastic leukaemia (ALL) is a malignant clonal disease, one of the most common malignancies in childhood. Contemporary protocols ensure high remission rate and long term free survival. The ability of molecular genetic methods help to establish submicroscopic classification a...
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| Format: | Article |
| Language: | English |
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Serbian Medical Society
2009-01-01
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| Series: | Srpski Arhiv za Celokupno Lekarstvo |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0370-8179/2009/0370-81790908384L.pdf |
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| author | Lazić Jelena Dokmanović Lidija Krstovski Nada Predojević Jelica Tošić Nataša Pavlović Sonja Janić Dragana |
| author_facet | Lazić Jelena Dokmanović Lidija Krstovski Nada Predojević Jelica Tošić Nataša Pavlović Sonja Janić Dragana |
| author_sort | Lazić Jelena |
| collection | DOAJ |
| description | Introduction. Acute lymphoblastic leukaemia (ALL) is a malignant clonal disease, one of the most common malignancies in childhood. Contemporary protocols ensure high remission rate and long term free survival. The ability of molecular genetic methods help to establish submicroscopic classification and minimal residual disease (MRD) follow up, in major percent responsible for relapse. Objective. The aim of the study was to detect the frequency of IgH and TCR gene rearrangements and their correlation with clinical parameters. Methods. Forty-one children with ALL were enrolled in the study group, with initial diagnosis of IgH and TCR gene rearrangements by polimerase chain reaction ( PCR). MRD follow-up was performed in induction phase when morphological remission was expected, and after intensive chemiotherapy. Results. In the study group IgH rearrangement was detected in 82.9% of children at the diagnosis, while TCR rearrangement was seen in 56.1%. On induction day 33, clonal IgH rearrangements persisted in 39% and TCR rearrangements in 36.5% of children. Conclusion. Molecular analysis of genetic alterations and their correlation with standard prognostic parameters show the importance of risk stratification revision which leads to new therapy intensification approach. MRD stands out as a precise predictive factor for the relapse of disease. |
| first_indexed | 2024-12-20T00:45:03Z |
| format | Article |
| id | doaj.art-06c17503c6504846a5289088e7578c4a |
| institution | Directory Open Access Journal |
| issn | 0370-8179 |
| language | English |
| last_indexed | 2024-12-20T00:45:03Z |
| publishDate | 2009-01-01 |
| publisher | Serbian Medical Society |
| record_format | Article |
| series | Srpski Arhiv za Celokupno Lekarstvo |
| spelling | doaj.art-06c17503c6504846a5289088e7578c4a2022-12-21T19:59:26ZengSerbian Medical SocietySrpski Arhiv za Celokupno Lekarstvo0370-81792009-01-011377-838439010.2298/SARH0908384LImmunoglobulin genes and T-cell receptors as molecular markers in children with acute lymphoblastic leukaemiaLazić JelenaDokmanović LidijaKrstovski NadaPredojević JelicaTošić NatašaPavlović SonjaJanić DraganaIntroduction. Acute lymphoblastic leukaemia (ALL) is a malignant clonal disease, one of the most common malignancies in childhood. Contemporary protocols ensure high remission rate and long term free survival. The ability of molecular genetic methods help to establish submicroscopic classification and minimal residual disease (MRD) follow up, in major percent responsible for relapse. Objective. The aim of the study was to detect the frequency of IgH and TCR gene rearrangements and their correlation with clinical parameters. Methods. Forty-one children with ALL were enrolled in the study group, with initial diagnosis of IgH and TCR gene rearrangements by polimerase chain reaction ( PCR). MRD follow-up was performed in induction phase when morphological remission was expected, and after intensive chemiotherapy. Results. In the study group IgH rearrangement was detected in 82.9% of children at the diagnosis, while TCR rearrangement was seen in 56.1%. On induction day 33, clonal IgH rearrangements persisted in 39% and TCR rearrangements in 36.5% of children. Conclusion. Molecular analysis of genetic alterations and their correlation with standard prognostic parameters show the importance of risk stratification revision which leads to new therapy intensification approach. MRD stands out as a precise predictive factor for the relapse of disease.http://www.doiserbia.nb.rs/img/doi/0370-8179/2009/0370-81790908384L.pdfacute lymphoblast leukaemiaminimal residual diseaseIgH and TCR gene rearrangements |
| spellingShingle | Lazić Jelena Dokmanović Lidija Krstovski Nada Predojević Jelica Tošić Nataša Pavlović Sonja Janić Dragana Immunoglobulin genes and T-cell receptors as molecular markers in children with acute lymphoblastic leukaemia Srpski Arhiv za Celokupno Lekarstvo acute lymphoblast leukaemia minimal residual disease IgH and TCR gene rearrangements |
| title | Immunoglobulin genes and T-cell receptors as molecular markers in children with acute lymphoblastic leukaemia |
| title_full | Immunoglobulin genes and T-cell receptors as molecular markers in children with acute lymphoblastic leukaemia |
| title_fullStr | Immunoglobulin genes and T-cell receptors as molecular markers in children with acute lymphoblastic leukaemia |
| title_full_unstemmed | Immunoglobulin genes and T-cell receptors as molecular markers in children with acute lymphoblastic leukaemia |
| title_short | Immunoglobulin genes and T-cell receptors as molecular markers in children with acute lymphoblastic leukaemia |
| title_sort | immunoglobulin genes and t cell receptors as molecular markers in children with acute lymphoblastic leukaemia |
| topic | acute lymphoblast leukaemia minimal residual disease IgH and TCR gene rearrangements |
| url | http://www.doiserbia.nb.rs/img/doi/0370-8179/2009/0370-81790908384L.pdf |
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