Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål)
SLC26A10 is a member of the SLC26 gene family, but its role in insects is still unclear. We cloned the SLC26A10 gene of Nilaparvata lugens (NlSLC26A10) and found NlSLC26A10 contained 11 transmembrane regions and a STAS domain. Expression pattern analysis showed NlSLC26A10 expression was more upregul...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-03-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphys.2022.853956/full |
| _version_ | 1818312899719856128 |
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| author | Ruijuan Zhang Jinliang Ji Yabin Li Jianbin Yu Xiaoping Yu Yipeng Xu |
| author_facet | Ruijuan Zhang Jinliang Ji Yabin Li Jianbin Yu Xiaoping Yu Yipeng Xu |
| author_sort | Ruijuan Zhang |
| collection | DOAJ |
| description | SLC26A10 is a member of the SLC26 gene family, but its role in insects is still unclear. We cloned the SLC26A10 gene of Nilaparvata lugens (NlSLC26A10) and found NlSLC26A10 contained 11 transmembrane regions and a STAS domain. Expression pattern analysis showed NlSLC26A10 expression was more upregulated in adults than in nymphs, highest in the ovary. After injection of double-stranded RNA (dsRNA) of NlSLC26A10, the mRNA level of NlSLC26A10 significantly decreased and, consequently, the ovarian development of adult females was hindered; the amount and the hatchability of eggs and yeast-like symbionts in mature oocytes decreased. Further study showed that NlSLC26A10 might result in decreased juvenile hormone level and vitellogenin expression. These results indicate that NlSLC26A10 plays an essential role in the reproduction of N. lugens. |
| first_indexed | 2024-12-13T08:25:11Z |
| format | Article |
| id | doaj.art-06c4ade3d5d7459a83d4a28b0b1d9449 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-13T08:25:11Z |
| publishDate | 2022-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-06c4ade3d5d7459a83d4a28b0b1d94492022-12-21T23:53:54ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2022-03-011310.3389/fphys.2022.853956853956Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål)Ruijuan ZhangJinliang JiYabin LiJianbin YuXiaoping YuYipeng XuSLC26A10 is a member of the SLC26 gene family, but its role in insects is still unclear. We cloned the SLC26A10 gene of Nilaparvata lugens (NlSLC26A10) and found NlSLC26A10 contained 11 transmembrane regions and a STAS domain. Expression pattern analysis showed NlSLC26A10 expression was more upregulated in adults than in nymphs, highest in the ovary. After injection of double-stranded RNA (dsRNA) of NlSLC26A10, the mRNA level of NlSLC26A10 significantly decreased and, consequently, the ovarian development of adult females was hindered; the amount and the hatchability of eggs and yeast-like symbionts in mature oocytes decreased. Further study showed that NlSLC26A10 might result in decreased juvenile hormone level and vitellogenin expression. These results indicate that NlSLC26A10 plays an essential role in the reproduction of N. lugens.https://www.frontiersin.org/articles/10.3389/fphys.2022.853956/fullNilaparvata lugensSLC26A10RNAiovarian developmentreproduction |
| spellingShingle | Ruijuan Zhang Jinliang Ji Yabin Li Jianbin Yu Xiaoping Yu Yipeng Xu Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål) Frontiers in Physiology Nilaparvata lugens SLC26A10 RNAi ovarian development reproduction |
| title | Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål) |
| title_full | Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål) |
| title_fullStr | Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål) |
| title_full_unstemmed | Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål) |
| title_short | Molecular Characterization and RNA Interference Analysis of SLC26A10 From Nilaparvata lugens (Stål) |
| title_sort | molecular characterization and rna interference analysis of slc26a10 from nilaparvata lugens stal |
| topic | Nilaparvata lugens SLC26A10 RNAi ovarian development reproduction |
| url | https://www.frontiersin.org/articles/10.3389/fphys.2022.853956/full |
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