The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAs
Non-coding RNA biogenesis in higher eukaryotes has not been fully characterized. Here, we studied the Drosophila melanogaster Rexo5 (CG8368) protein, a metazoan-specific member of the DEDDh 3′-5′ single-stranded RNA exonucleases, by genetic, biochemical, and RNA-sequencing approaches. Rexo5 is requi...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-10-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124717313724 |
| _version_ | 1819262830413611008 |
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| author | Stefanie Gerstberger Cindy Meyer Sigi Benjamin-Hong Joe Rodriguez Daniel Briskin Claudia Bognanni Kimberly Bogardus Hermann Steller Thomas Tuschl |
| author_facet | Stefanie Gerstberger Cindy Meyer Sigi Benjamin-Hong Joe Rodriguez Daniel Briskin Claudia Bognanni Kimberly Bogardus Hermann Steller Thomas Tuschl |
| author_sort | Stefanie Gerstberger |
| collection | DOAJ |
| description | Non-coding RNA biogenesis in higher eukaryotes has not been fully characterized. Here, we studied the Drosophila melanogaster Rexo5 (CG8368) protein, a metazoan-specific member of the DEDDh 3′-5′ single-stranded RNA exonucleases, by genetic, biochemical, and RNA-sequencing approaches. Rexo5 is required for small nucleolar RNA (snoRNA) and rRNA biogenesis and is essential in D. melanogaster. Loss-of-function mutants accumulate improperly 3′ end-trimmed 28S rRNA, 5S rRNA, and snoRNA precursors in vivo. Rexo5 is ubiquitously expressed at low levels in somatic metazoan cells but extremely elevated in male and female germ cells. Loss of Rexo5 leads to increased nucleolar size, genomic instability, defective ribosome subunit export, and larval death. Loss of germline expression compromises gonadal growth and meiotic entry during germline development. |
| first_indexed | 2024-12-23T20:03:56Z |
| format | Article |
| id | doaj.art-06c6c57008cb4839b64703f881b49613 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-23T20:03:56Z |
| publishDate | 2017-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-06c6c57008cb4839b64703f881b496132022-12-21T17:33:00ZengElsevierCell Reports2211-12472017-10-0121375877210.1016/j.celrep.2017.09.067The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAsStefanie Gerstberger0Cindy Meyer1Sigi Benjamin-Hong2Joe Rodriguez3Daniel Briskin4Claudia Bognanni5Kimberly Bogardus6Hermann Steller7Thomas Tuschl8Howard Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY 10065, USAHoward Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY 10065, USAStrang Laboratory of Apoptosis and Cancer Biology, The Rockefeller University, New York, NY 10065, USAStrang Laboratory of Apoptosis and Cancer Biology, The Rockefeller University, New York, NY 10065, USAHoward Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY 10065, USAHoward Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY 10065, USAHoward Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY 10065, USAStrang Laboratory of Apoptosis and Cancer Biology, The Rockefeller University, New York, NY 10065, USAHoward Hughes Medical Institute, Laboratory of RNA Molecular Biology, The Rockefeller University, New York, NY 10065, USANon-coding RNA biogenesis in higher eukaryotes has not been fully characterized. Here, we studied the Drosophila melanogaster Rexo5 (CG8368) protein, a metazoan-specific member of the DEDDh 3′-5′ single-stranded RNA exonucleases, by genetic, biochemical, and RNA-sequencing approaches. Rexo5 is required for small nucleolar RNA (snoRNA) and rRNA biogenesis and is essential in D. melanogaster. Loss-of-function mutants accumulate improperly 3′ end-trimmed 28S rRNA, 5S rRNA, and snoRNA precursors in vivo. Rexo5 is ubiquitously expressed at low levels in somatic metazoan cells but extremely elevated in male and female germ cells. Loss of Rexo5 leads to increased nucleolar size, genomic instability, defective ribosome subunit export, and larval death. Loss of germline expression compromises gonadal growth and meiotic entry during germline development.http://www.sciencedirect.com/science/article/pii/S2211124717313724rRNA biogenesisrRNA 3′ end maturationrRNA processingsnoRNA biogenesissnoRNA 3′ end maturationsnoRNA processingRNA exonucleaseU8 snoRNA |
| spellingShingle | Stefanie Gerstberger Cindy Meyer Sigi Benjamin-Hong Joe Rodriguez Daniel Briskin Claudia Bognanni Kimberly Bogardus Hermann Steller Thomas Tuschl The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAs Cell Reports rRNA biogenesis rRNA 3′ end maturation rRNA processing snoRNA biogenesis snoRNA 3′ end maturation snoRNA processing RNA exonuclease U8 snoRNA |
| title | The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAs |
| title_full | The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAs |
| title_fullStr | The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAs |
| title_full_unstemmed | The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAs |
| title_short | The Conserved RNA Exonuclease Rexo5 Is Required for 3′ End Maturation of 28S rRNA, 5S rRNA, and snoRNAs |
| title_sort | conserved rna exonuclease rexo5 is required for 3 end maturation of 28s rrna 5s rrna and snornas |
| topic | rRNA biogenesis rRNA 3′ end maturation rRNA processing snoRNA biogenesis snoRNA 3′ end maturation snoRNA processing RNA exonuclease U8 snoRNA |
| url | http://www.sciencedirect.com/science/article/pii/S2211124717313724 |
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