Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo
In an effort to find topical agents that prevent or retard cutaneous aging, seven functional lipids were screened for their procollagen-upregulating and matrix metalloproteinase (MMP)-1-downregulating activities in human dermal fibroblasts by Western blotting. The preventive effect on ultraviolet (U...
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| Format: | Article |
| Language: | English |
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Elsevier
2008-06-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520423685 |
| _version_ | 1818920460626165760 |
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| author | Soyun Cho Hyeon Ho Kim Min Jung Lee Serah Lee Chang-Seo Park Sang-June Nam Jeong-Jun Han Jin-Wook Kim Jin Ho Chung |
| author_facet | Soyun Cho Hyeon Ho Kim Min Jung Lee Serah Lee Chang-Seo Park Sang-June Nam Jeong-Jun Han Jin-Wook Kim Jin Ho Chung |
| author_sort | Soyun Cho |
| collection | DOAJ |
| description | In an effort to find topical agents that prevent or retard cutaneous aging, seven functional lipids were screened for their procollagen-upregulating and matrix metalloproteinase (MMP)-1-downregulating activities in human dermal fibroblasts by Western blotting. The preventive effect on ultraviolet (UV)-induced decrease of procollagen was demonstrated in phosphatidylserine (PS), lysophosphatidylserine (LPS), lysophosphatidic acid (LPA), N-acetyl phytosphingosine (NAPS), and tetraacetyl phytosphingosine (TAPS). Furthermore, PS, LPS, and LPA upregulated procollagen expression in unirradiated basal conditions. The inhibitory effect on UV-induced MMP-1 expression was seen in NAPS, TAPS, LPA, PS, lysophosphatidylglycerol, and LPS. PS was chosen as the most suitable candidate anti-aging chemical for the subsequent in vivo studies. We investigated the effects of PS on acute UV response and chronologic skin aging by topically applying it to young skin before UV irradiation and to aged human skin, respectively. Real-time PCR and Western blot revealed that in the young skin, PS treatment prevented UV-induced reduction in procollagen expression and inhibited UV-induced MMP-1 expression. PS also blocked UV-induced IL-6 and COX-2 gene expression in cultured fibroblasts dose-dependently. In the aged skin, PS caused increased procollagen transcription and procollagen immunostaining in the upper dermis, and a significant decrease in MMP-1 expression at both mRNA and protein levels. These results indicate that topical PS has anti-skin-aging properties and point to the potential use of PS as a therapeutic agent in the prevention and treatment of cutaneous aging. |
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| id | doaj.art-06c6db71dafd4c99bd9c70162904bdef |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-20T01:22:06Z |
| publishDate | 2008-06-01 |
| publisher | Elsevier |
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| series | Journal of Lipid Research |
| spelling | doaj.art-06c6db71dafd4c99bd9c70162904bdef2022-12-21T19:58:23ZengElsevierJournal of Lipid Research0022-22752008-06-0149612351245Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivoSoyun Cho0Hyeon Ho Kim1Min Jung Lee2Serah Lee3Chang-Seo Park4Sang-June Nam5Jeong-Jun Han6Jin-Wook Kim7Jin Ho Chung8Department of Dermatology, Seoul National University College of Medicine, Seoul, Korea; Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea; Institute of Dermatological Science, Seoul, Korea; Boramae Hospital, Seoul National University, Seoul, KoreaDepartment of Dermatology, Seoul National University College of Medicine, Seoul, Korea; Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea; Institute of Dermatological Science, Seoul, KoreaDepartment of Dermatology, Seoul National University College of Medicine, Seoul, Korea; Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea; Institute of Dermatological Science, Seoul, KoreaDepartment of Dermatology, Seoul National University College of Medicine, Seoul, Korea; Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea; Institute of Dermatological Science, Seoul, KoreaDepartment of Chemical and Biochemical Engineering, Dongguk University, Seoul, KoreaDoosan Biotech Division, Gyeonggido, KoreaDoosan Biotech Division, Gyeonggido, KoreaDoosan Biotech Division, Gyeonggido, KoreaDepartment of Dermatology, Seoul National University College of Medicine, Seoul, Korea; Laboratory of Cutaneous Aging Research, Clinical Research Institute, Seoul National University Hospital, Seoul, Korea; Institute of Dermatological Science, Seoul, KoreaIn an effort to find topical agents that prevent or retard cutaneous aging, seven functional lipids were screened for their procollagen-upregulating and matrix metalloproteinase (MMP)-1-downregulating activities in human dermal fibroblasts by Western blotting. The preventive effect on ultraviolet (UV)-induced decrease of procollagen was demonstrated in phosphatidylserine (PS), lysophosphatidylserine (LPS), lysophosphatidic acid (LPA), N-acetyl phytosphingosine (NAPS), and tetraacetyl phytosphingosine (TAPS). Furthermore, PS, LPS, and LPA upregulated procollagen expression in unirradiated basal conditions. The inhibitory effect on UV-induced MMP-1 expression was seen in NAPS, TAPS, LPA, PS, lysophosphatidylglycerol, and LPS. PS was chosen as the most suitable candidate anti-aging chemical for the subsequent in vivo studies. We investigated the effects of PS on acute UV response and chronologic skin aging by topically applying it to young skin before UV irradiation and to aged human skin, respectively. Real-time PCR and Western blot revealed that in the young skin, PS treatment prevented UV-induced reduction in procollagen expression and inhibited UV-induced MMP-1 expression. PS also blocked UV-induced IL-6 and COX-2 gene expression in cultured fibroblasts dose-dependently. In the aged skin, PS caused increased procollagen transcription and procollagen immunostaining in the upper dermis, and a significant decrease in MMP-1 expression at both mRNA and protein levels. These results indicate that topical PS has anti-skin-aging properties and point to the potential use of PS as a therapeutic agent in the prevention and treatment of cutaneous aging.http://www.sciencedirect.com/science/article/pii/S0022227520423685ultravioletintrinsic agingphotoagingmatrix metalloproteinase-1 |
| spellingShingle | Soyun Cho Hyeon Ho Kim Min Jung Lee Serah Lee Chang-Seo Park Sang-June Nam Jeong-Jun Han Jin-Wook Kim Jin Ho Chung Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo Journal of Lipid Research ultraviolet intrinsic aging photoaging matrix metalloproteinase-1 |
| title | Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo |
| title_full | Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo |
| title_fullStr | Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo |
| title_full_unstemmed | Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo |
| title_short | Phosphatidylserine prevents UV-induced decrease of type I procollagen and increase of MMP-1 in dermal fibroblasts and human skin in vivo |
| title_sort | phosphatidylserine prevents uv induced decrease of type i procollagen and increase of mmp 1 in dermal fibroblasts and human skin in vivo |
| topic | ultraviolet intrinsic aging photoaging matrix metalloproteinase-1 |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520423685 |
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