The Pta-AckA Pathway Regulates LrgAB-Mediated Pyruvate Uptake in <i>Streptococcus mutans</i>
Pyruvate forms the central node of carbon metabolism and promotes growth as an alternative carbon source during starvation. We recently revealed that LrgAB functions as a stationary phase pyruvate uptake system in <i>Streptococcus mutans,</i> the primary causative agent of human dental c...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/6/846 |
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| author | Sang-Joon Ahn Shailja Desai Min Lin Kelly C. Rice |
| author_facet | Sang-Joon Ahn Shailja Desai Min Lin Kelly C. Rice |
| author_sort | Sang-Joon Ahn |
| collection | DOAJ |
| description | Pyruvate forms the central node of carbon metabolism and promotes growth as an alternative carbon source during starvation. We recently revealed that LrgAB functions as a stationary phase pyruvate uptake system in <i>Streptococcus mutans,</i> the primary causative agent of human dental caries, but its underlying regulatory mechanisms are still not clearly understood. This study was aimed at further characterizing the regulation of LrgAB from a metabolomic perspective. We utilized a series of GFP quantification, growth kinetics, and biochemical assays. We disclosed that LrgAB is critical for pyruvate uptake especially during growth under low-glucose stress. Inactivation of the Pta-Ack pathway, responsible for the conversion of acetyl-CoA to acetate, completely inhibits stationary phase <i>lrgAB</i> induction and pyruvate uptake, and renders cells insensitive to external pyruvate as a signal. Inactivation of Pfl, responsible for the conversion of pyruvate to acetyl-CoA under anaerobic conditions, also affected stationary phase pyruvate uptake. This study explores the metabolic components of pyruvate uptake regulation through LrgAB, and highlights its potential as a metabolic stimulator, contributing to the resuscitation and survival of <i>S. mutans</i> cells during nutritional stress. |
| first_indexed | 2024-03-10T19:22:24Z |
| format | Article |
| id | doaj.art-06c6de464266484382e7c2cdb87e8642 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T19:22:24Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-06c6de464266484382e7c2cdb87e86422023-11-20T02:51:21ZengMDPI AGMicroorganisms2076-26072020-06-018684610.3390/microorganisms8060846The Pta-AckA Pathway Regulates LrgAB-Mediated Pyruvate Uptake in <i>Streptococcus mutans</i>Sang-Joon Ahn0Shailja Desai1Min Lin2Kelly C. Rice3Department of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Oral Biology, College of Dentistry, University of Florida, Gainesville, FL 32610, USADepartment of Chemistry, College of Arts and Sciences, University of Florida, Gainesville, FL 32610, USADepartment of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, Gainesville, FL 32610, USAPyruvate forms the central node of carbon metabolism and promotes growth as an alternative carbon source during starvation. We recently revealed that LrgAB functions as a stationary phase pyruvate uptake system in <i>Streptococcus mutans,</i> the primary causative agent of human dental caries, but its underlying regulatory mechanisms are still not clearly understood. This study was aimed at further characterizing the regulation of LrgAB from a metabolomic perspective. We utilized a series of GFP quantification, growth kinetics, and biochemical assays. We disclosed that LrgAB is critical for pyruvate uptake especially during growth under low-glucose stress. Inactivation of the Pta-Ack pathway, responsible for the conversion of acetyl-CoA to acetate, completely inhibits stationary phase <i>lrgAB</i> induction and pyruvate uptake, and renders cells insensitive to external pyruvate as a signal. Inactivation of Pfl, responsible for the conversion of pyruvate to acetyl-CoA under anaerobic conditions, also affected stationary phase pyruvate uptake. This study explores the metabolic components of pyruvate uptake regulation through LrgAB, and highlights its potential as a metabolic stimulator, contributing to the resuscitation and survival of <i>S. mutans</i> cells during nutritional stress.https://www.mdpi.com/2076-2607/8/6/846<i>Streptococcus mutans</i>LrgABpyruvateacetyl-CoAPtaAckA |
| spellingShingle | Sang-Joon Ahn Shailja Desai Min Lin Kelly C. Rice The Pta-AckA Pathway Regulates LrgAB-Mediated Pyruvate Uptake in <i>Streptococcus mutans</i> Microorganisms <i>Streptococcus mutans</i> LrgAB pyruvate acetyl-CoA Pta AckA |
| title | The Pta-AckA Pathway Regulates LrgAB-Mediated Pyruvate Uptake in <i>Streptococcus mutans</i> |
| title_full | The Pta-AckA Pathway Regulates LrgAB-Mediated Pyruvate Uptake in <i>Streptococcus mutans</i> |
| title_fullStr | The Pta-AckA Pathway Regulates LrgAB-Mediated Pyruvate Uptake in <i>Streptococcus mutans</i> |
| title_full_unstemmed | The Pta-AckA Pathway Regulates LrgAB-Mediated Pyruvate Uptake in <i>Streptococcus mutans</i> |
| title_short | The Pta-AckA Pathway Regulates LrgAB-Mediated Pyruvate Uptake in <i>Streptococcus mutans</i> |
| title_sort | pta acka pathway regulates lrgab mediated pyruvate uptake in i streptococcus mutans i |
| topic | <i>Streptococcus mutans</i> LrgAB pyruvate acetyl-CoA Pta AckA |
| url | https://www.mdpi.com/2076-2607/8/6/846 |
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