A 4D Proteome Investigation of the Potential Mechanisms of SA in Triggering Resistance in Kiwifruit to <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
Kiwifruit bacterial cankers caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) are a serious threat to the kiwifruit industry. Salicylic acid (SA) regulates plant defense responses and was previously found to enhance kiwifruit’s resistance to Psa. However, the...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/24/17448 |
| _version_ | 1797380660013301760 |
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| author | Dong Qu Fei Yan Yu Zhang Lili Huang |
| author_facet | Dong Qu Fei Yan Yu Zhang Lili Huang |
| author_sort | Dong Qu |
| collection | DOAJ |
| description | Kiwifruit bacterial cankers caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) are a serious threat to the kiwifruit industry. Salicylic acid (SA) regulates plant defense responses and was previously found to enhance kiwifruit’s resistance to Psa. However, the underlying mechanisms of this process remain unclear. In this study, we used 4D proteomics to investigate how SA enhances kiwifruit’s resistance to Psa and found that both SA treatment and Psa infection induced dramatic changes in the proteomic pattern of kiwifruit. Psa infection triggered the activation of numerous resistance events, including the MAPK cascade, phenylpropanoid biosynthesis, and hormone signaling transduction. In most cases, the differential expression of a number of genes involved in the SA signaling pathway played a significant role in kiwifruit’s responses to Psa. Moreover, SA treatment upregulated numerous resistance-related proteins, which functioned in defense responses to Psa, including phenylpropanoid biosynthesis, the MAPK cascade, and the upregulation of pathogenesis-related proteins. We also found that SA treatment could facilitate timely defense responses to Psa infection and enhance the activation of defense responses that were downregulated in kiwifruit during infection with Psa. Thus, our research deciphered the potential mechanisms of SA in promoting Psa resistance in kiwifruit and can provide a basis for the use of SA to enhance kiwifruit resistance and effectively control the occurrence of kiwifruit bacterial cankers. |
| first_indexed | 2024-03-08T20:41:25Z |
| format | Article |
| id | doaj.art-06caafee8e17480e831f6f78597b1a5a |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T20:41:25Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-06caafee8e17480e831f6f78597b1a5a2023-12-22T14:14:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0124241744810.3390/ijms242417448A 4D Proteome Investigation of the Potential Mechanisms of SA in Triggering Resistance in Kiwifruit to <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>Dong Qu0Fei Yan1Yu Zhang2Lili Huang3College of Plant Protection, Northwest A&F University, Xianyang 712100, ChinaShaanxi Provincial Bioresource Key Laboratory, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaShaanxi Provincial Bioresource Key Laboratory, College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Plant Protection, Northwest A&F University, Xianyang 712100, ChinaKiwifruit bacterial cankers caused by <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) are a serious threat to the kiwifruit industry. Salicylic acid (SA) regulates plant defense responses and was previously found to enhance kiwifruit’s resistance to Psa. However, the underlying mechanisms of this process remain unclear. In this study, we used 4D proteomics to investigate how SA enhances kiwifruit’s resistance to Psa and found that both SA treatment and Psa infection induced dramatic changes in the proteomic pattern of kiwifruit. Psa infection triggered the activation of numerous resistance events, including the MAPK cascade, phenylpropanoid biosynthesis, and hormone signaling transduction. In most cases, the differential expression of a number of genes involved in the SA signaling pathway played a significant role in kiwifruit’s responses to Psa. Moreover, SA treatment upregulated numerous resistance-related proteins, which functioned in defense responses to Psa, including phenylpropanoid biosynthesis, the MAPK cascade, and the upregulation of pathogenesis-related proteins. We also found that SA treatment could facilitate timely defense responses to Psa infection and enhance the activation of defense responses that were downregulated in kiwifruit during infection with Psa. Thus, our research deciphered the potential mechanisms of SA in promoting Psa resistance in kiwifruit and can provide a basis for the use of SA to enhance kiwifruit resistance and effectively control the occurrence of kiwifruit bacterial cankers.https://www.mdpi.com/1422-0067/24/24/17448kiwifruit plantsalicylic acidbacterial cankerproteome |
| spellingShingle | Dong Qu Fei Yan Yu Zhang Lili Huang A 4D Proteome Investigation of the Potential Mechanisms of SA in Triggering Resistance in Kiwifruit to <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> International Journal of Molecular Sciences kiwifruit plant salicylic acid bacterial canker proteome |
| title | A 4D Proteome Investigation of the Potential Mechanisms of SA in Triggering Resistance in Kiwifruit to <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_full | A 4D Proteome Investigation of the Potential Mechanisms of SA in Triggering Resistance in Kiwifruit to <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_fullStr | A 4D Proteome Investigation of the Potential Mechanisms of SA in Triggering Resistance in Kiwifruit to <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_full_unstemmed | A 4D Proteome Investigation of the Potential Mechanisms of SA in Triggering Resistance in Kiwifruit to <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_short | A 4D Proteome Investigation of the Potential Mechanisms of SA in Triggering Resistance in Kiwifruit to <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> |
| title_sort | 4d proteome investigation of the potential mechanisms of sa in triggering resistance in kiwifruit to i pseudomonas syringae i pv i actinidiae i |
| topic | kiwifruit plant salicylic acid bacterial canker proteome |
| url | https://www.mdpi.com/1422-0067/24/24/17448 |
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