Fecal carriage and phylo-diversity of community-acquired blaTEM Enteric bacilli in Southwest Nigeria
Paul Akinniyi Akinduti,1 Grace Iyabo Olasehinde,1 Oluwaseun Ejilude,2 Olugbenga Samson Taiwo,1 Yemisi Dorcas Obafemi1 1Microbiology Unit, Department of Biological Sciences, Covenant University, Otta, Nigeria; 2Microbiology Unit, Sacred Heart Hospital, Lantoro, Abeokuta, Nigeria Purpose: Increasing r...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2018-11-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/fecal-carriage-and-phylo-diversity-of-community-acquired-blatem-enteri-peer-reviewed-article-IDR |
| _version_ | 1818574422438576128 |
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| author | Akinduti PA Olasehinde GI Ejilude O Taiwo OS Obafemi YD |
| author_facet | Akinduti PA Olasehinde GI Ejilude O Taiwo OS Obafemi YD |
| author_sort | Akinduti PA |
| collection | DOAJ |
| description | Paul Akinniyi Akinduti,1 Grace Iyabo Olasehinde,1 Oluwaseun Ejilude,2 Olugbenga Samson Taiwo,1 Yemisi Dorcas Obafemi1 1Microbiology Unit, Department of Biological Sciences, Covenant University, Otta, Nigeria; 2Microbiology Unit, Sacred Heart Hospital, Lantoro, Abeokuta, Nigeria Purpose: Increasing rates of clonal spread of fecal blaTEM bacilli remains a huge concern to the community health with resultant high morbidity. The fecal carriage and clonal diversity of blaTEM within the communities in Southwest Nigeria were surveyed.Materials and methods: Enteric bacilli obtained from fresh fecal samples randomly collected from community residents were biotyped and profiled for antibiotic susceptibility. Resistant strains were typed for beta-lactamase, extended-spectrum beta-lactamases (ESBL), AmpC and carbapenemase production while the R-plasmid carriage was detected and mating activities were examined. The presence of blaTEM gene was assayed by PCR and its phylodiversity determined with 16sRNA genomic profiling.Results: Escherichia coli have the highest (28.6%) occurrence rate and Pseudomonas aeruginosa (20.5%) showing significant resistance to beta-lactamase inhibitors (ampicillin, cefuroxime and cefotaxime), and high-level multidrug resistance of more than 15.2% rate to ampicillin, cefuroxime, ceftazidime, tetracycline and imipenem. E. coli and Klebsiella oxytoca, are the highest beta-lactamase, ESBL and AmpC producers encoded with high molecular weight R-plasmid (>11.0 kbp) and significant rate of conjugation and transformational activities. Only 2/14, 1/13 and 1/6 ESBL-type of E. coli, K. oxytoca and Enterobacter cloaca, expressed blaTEM gene, clustering into five different phylodiverse groups with close genomic relatedness with other bacilli.Conclusion: This is an indication of clonal dissemination of ESBL blaTEM encoded enteric bacilli having high phylodiverse characteristics through fecal carriage in the Nigerian community which requires public health education, food and environmental hygiene for its prevention. Keywords: fecal, antibiotics, ESBL, R-plasmid, blaTEM |
| first_indexed | 2024-12-15T00:26:27Z |
| format | Article |
| id | doaj.art-06cd9c96b48944768c608c7c5725ab1f |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-12-15T00:26:27Z |
| publishDate | 2018-11-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-06cd9c96b48944768c608c7c5725ab1f2022-12-21T22:42:09ZengDove Medical PressInfection and Drug Resistance1178-69732018-11-01Volume 112425243342639Fecal carriage and phylo-diversity of community-acquired blaTEM Enteric bacilli in Southwest NigeriaAkinduti PAOlasehinde GIEjilude OTaiwo OSObafemi YDPaul Akinniyi Akinduti,1 Grace Iyabo Olasehinde,1 Oluwaseun Ejilude,2 Olugbenga Samson Taiwo,1 Yemisi Dorcas Obafemi1 1Microbiology Unit, Department of Biological Sciences, Covenant University, Otta, Nigeria; 2Microbiology Unit, Sacred Heart Hospital, Lantoro, Abeokuta, Nigeria Purpose: Increasing rates of clonal spread of fecal blaTEM bacilli remains a huge concern to the community health with resultant high morbidity. The fecal carriage and clonal diversity of blaTEM within the communities in Southwest Nigeria were surveyed.Materials and methods: Enteric bacilli obtained from fresh fecal samples randomly collected from community residents were biotyped and profiled for antibiotic susceptibility. Resistant strains were typed for beta-lactamase, extended-spectrum beta-lactamases (ESBL), AmpC and carbapenemase production while the R-plasmid carriage was detected and mating activities were examined. The presence of blaTEM gene was assayed by PCR and its phylodiversity determined with 16sRNA genomic profiling.Results: Escherichia coli have the highest (28.6%) occurrence rate and Pseudomonas aeruginosa (20.5%) showing significant resistance to beta-lactamase inhibitors (ampicillin, cefuroxime and cefotaxime), and high-level multidrug resistance of more than 15.2% rate to ampicillin, cefuroxime, ceftazidime, tetracycline and imipenem. E. coli and Klebsiella oxytoca, are the highest beta-lactamase, ESBL and AmpC producers encoded with high molecular weight R-plasmid (>11.0 kbp) and significant rate of conjugation and transformational activities. Only 2/14, 1/13 and 1/6 ESBL-type of E. coli, K. oxytoca and Enterobacter cloaca, expressed blaTEM gene, clustering into five different phylodiverse groups with close genomic relatedness with other bacilli.Conclusion: This is an indication of clonal dissemination of ESBL blaTEM encoded enteric bacilli having high phylodiverse characteristics through fecal carriage in the Nigerian community which requires public health education, food and environmental hygiene for its prevention. Keywords: fecal, antibiotics, ESBL, R-plasmid, blaTEMhttps://www.dovepress.com/fecal-carriage-and-phylo-diversity-of-community-acquired-blatem-enteri-peer-reviewed-article-IDRfecalantibioticsESBLR-plasmidblaTEM |
| spellingShingle | Akinduti PA Olasehinde GI Ejilude O Taiwo OS Obafemi YD Fecal carriage and phylo-diversity of community-acquired blaTEM Enteric bacilli in Southwest Nigeria Infection and Drug Resistance fecal antibiotics ESBL R-plasmid blaTEM |
| title | Fecal carriage and phylo-diversity of community-acquired blaTEM Enteric bacilli in Southwest Nigeria |
| title_full | Fecal carriage and phylo-diversity of community-acquired blaTEM Enteric bacilli in Southwest Nigeria |
| title_fullStr | Fecal carriage and phylo-diversity of community-acquired blaTEM Enteric bacilli in Southwest Nigeria |
| title_full_unstemmed | Fecal carriage and phylo-diversity of community-acquired blaTEM Enteric bacilli in Southwest Nigeria |
| title_short | Fecal carriage and phylo-diversity of community-acquired blaTEM Enteric bacilli in Southwest Nigeria |
| title_sort | fecal carriage and phylo diversity of community acquired blatem enteric bacilli in southwest nigeria |
| topic | fecal antibiotics ESBL R-plasmid blaTEM |
| url | https://www.dovepress.com/fecal-carriage-and-phylo-diversity-of-community-acquired-blatem-enteri-peer-reviewed-article-IDR |
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