Endophytic Fungi of Native Salvia abrotanoides Plants Reveal High Taxonomic Diversity and Unique Profiles of Secondary Metabolites
Endophytic fungi are often embedded in their host’s metabolic networks, which can result in alterations of metabolite production and higher amounts of active compounds in medicinal plants. This study reports the occurrence, diversity, and secondary metabolite profiles of endophytic fungi isolated fr...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.03013/full |
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| author | Yeganeh Teimoori-Boghsani Ali Ganjeali Tomislav Cernava Henry Müller Javad Asili Gabriele Berg |
| author_facet | Yeganeh Teimoori-Boghsani Ali Ganjeali Tomislav Cernava Henry Müller Javad Asili Gabriele Berg |
| author_sort | Yeganeh Teimoori-Boghsani |
| collection | DOAJ |
| description | Endophytic fungi are often embedded in their host’s metabolic networks, which can result in alterations of metabolite production and higher amounts of active compounds in medicinal plants. This study reports the occurrence, diversity, and secondary metabolite profiles of endophytic fungi isolated from Salvia abrotanoides plants obtained from three geographically distinct sites in Iran. A total of 56 endophytic fungi were isolated from roots and leaves of S. abrotanoides; site-specificity and root-dominated colonization was found to be a general characteristic of the endophytes. Based on molecular identification, the endophytic fungi were classified into 15 genera. Mycelial extracts of these isolates were subjected to high-resolution mass spectrometry analyses and revealed a broad spectrum of secondary metabolites. Our results demonstrated that Penicillium canescens, P. murcianum, Paraphoma radicina, and Coniolariella hispanica are producers of cryptotanshinone, which is a main bioactive compound of S. abrotanoides. Moreover, it was shown that it can be produced independent of the host plant. The effect of exogenous gibberellin on S. abrotanoides and endophytic fungi was shown to have a positive effect on increasing the cryptotanshinone production in the plant as well as in endophytic fungi cultivated under axenic conditions. Our findings provide further evidence that endophytic fungi play an important role in the production plant bioactive metabolites. Moreover, they provide an exploitable basis to increase cryptotanshinone production in S. abrotanoides. |
| first_indexed | 2024-12-12T17:01:18Z |
| format | Article |
| id | doaj.art-06cdaac894434055854c4b778ec3d850 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T17:01:18Z |
| publishDate | 2020-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-06cdaac894434055854c4b778ec3d8502022-12-22T00:18:08ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-01-011010.3389/fmicb.2019.03013490271Endophytic Fungi of Native Salvia abrotanoides Plants Reveal High Taxonomic Diversity and Unique Profiles of Secondary MetabolitesYeganeh Teimoori-Boghsani0Ali Ganjeali1Tomislav Cernava2Henry Müller3Javad Asili4Gabriele Berg5Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranDepartment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranInstitute of Environmental Biotechnology, Graz University of Technology, Graz, AustriaInstitute of Environmental Biotechnology, Graz University of Technology, Graz, AustriaDepartment of Pharmacognosy, Faculty of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IranInstitute of Environmental Biotechnology, Graz University of Technology, Graz, AustriaEndophytic fungi are often embedded in their host’s metabolic networks, which can result in alterations of metabolite production and higher amounts of active compounds in medicinal plants. This study reports the occurrence, diversity, and secondary metabolite profiles of endophytic fungi isolated from Salvia abrotanoides plants obtained from three geographically distinct sites in Iran. A total of 56 endophytic fungi were isolated from roots and leaves of S. abrotanoides; site-specificity and root-dominated colonization was found to be a general characteristic of the endophytes. Based on molecular identification, the endophytic fungi were classified into 15 genera. Mycelial extracts of these isolates were subjected to high-resolution mass spectrometry analyses and revealed a broad spectrum of secondary metabolites. Our results demonstrated that Penicillium canescens, P. murcianum, Paraphoma radicina, and Coniolariella hispanica are producers of cryptotanshinone, which is a main bioactive compound of S. abrotanoides. Moreover, it was shown that it can be produced independent of the host plant. The effect of exogenous gibberellin on S. abrotanoides and endophytic fungi was shown to have a positive effect on increasing the cryptotanshinone production in the plant as well as in endophytic fungi cultivated under axenic conditions. Our findings provide further evidence that endophytic fungi play an important role in the production plant bioactive metabolites. Moreover, they provide an exploitable basis to increase cryptotanshinone production in S. abrotanoides.https://www.frontiersin.org/article/10.3389/fmicb.2019.03013/fullSalvia abrotanoidesendophytic fungisecondary metabolitescryptotanshinonegibberellin |
| spellingShingle | Yeganeh Teimoori-Boghsani Ali Ganjeali Tomislav Cernava Henry Müller Javad Asili Gabriele Berg Endophytic Fungi of Native Salvia abrotanoides Plants Reveal High Taxonomic Diversity and Unique Profiles of Secondary Metabolites Frontiers in Microbiology Salvia abrotanoides endophytic fungi secondary metabolites cryptotanshinone gibberellin |
| title | Endophytic Fungi of Native Salvia abrotanoides Plants Reveal High Taxonomic Diversity and Unique Profiles of Secondary Metabolites |
| title_full | Endophytic Fungi of Native Salvia abrotanoides Plants Reveal High Taxonomic Diversity and Unique Profiles of Secondary Metabolites |
| title_fullStr | Endophytic Fungi of Native Salvia abrotanoides Plants Reveal High Taxonomic Diversity and Unique Profiles of Secondary Metabolites |
| title_full_unstemmed | Endophytic Fungi of Native Salvia abrotanoides Plants Reveal High Taxonomic Diversity and Unique Profiles of Secondary Metabolites |
| title_short | Endophytic Fungi of Native Salvia abrotanoides Plants Reveal High Taxonomic Diversity and Unique Profiles of Secondary Metabolites |
| title_sort | endophytic fungi of native salvia abrotanoides plants reveal high taxonomic diversity and unique profiles of secondary metabolites |
| topic | Salvia abrotanoides endophytic fungi secondary metabolites cryptotanshinone gibberellin |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.03013/full |
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