Minimum Inhibitory Concentration of Peganum Harmala Extract Against Candida Species
Background and Aim: There are some rich sources of antifungals in medicinal herbs. They have general acceptance due to their low cost and insignificant side effects. The efficacy of Peganum harmala (P. harmala) extract against Candida species was investigated in this study. Materials and Metho...
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| Format: | Article |
| Language: | English |
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Islamic Azad University
2022-07-01
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| Series: | Journal of Research in Dental and Maxillofacial Sciences |
| Subjects: | |
| Online Access: | http://jrdms.dentaliau.ac.ir/article-1-348-en.html |
| _version_ | 1818477517518929920 |
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| author | M Baharvand M Hakemi-Vala M Mehdipour L Dehghani E Jalali F Anbari |
| author_facet | M Baharvand M Hakemi-Vala M Mehdipour L Dehghani E Jalali F Anbari |
| author_sort | M Baharvand |
| collection | DOAJ |
| description | Background and Aim: There are some rich sources of antifungals in medicinal herbs. They have general acceptance due to their low cost and insignificant side effects. The efficacy of Peganum harmala (P. harmala) extract against Candida species was investigated in this study.
Materials and Methods: In this in vitro study, we used the maceration technique to obtain P. harmala aqueous and alcoholic extracts, and assess their antifungal activity against different species of Candida albicans (C. albicans), Candida glabrata (C. glabrata) and Candida krusei (C. krusei). We used the microdilution method to calculate the minimum inhibitory concentration (MIC). The tests were repeated in triplicate for higher accuracy.
Results: The aqueous and alcoholic P. harmala extracts showed antifungal activity against C. albicans in 100 mg/mL concentration. The antifungal efficacy of P. harmala alcoholic extract against C.
glabrata (MIC: 100 mg/mL) was higher than the aqueous extract (MIC:200 mg/mL). The antifungal efficacy of P. harmala alcoholic extract against C. krusei (MIC: 1.56 mg/mL) was higher than the aqueous extract (MIC: 12.5 mg/mL).
Conclusion: The aqueous and alcoholic extracts of P. harmala were effective against all species of Candida particularly Candida krusei. The alcoholic extract of P. harmala was more effective than its aqueous extract against C. glabrata and C. krusei. |
| first_indexed | 2024-12-10T09:38:42Z |
| format | Article |
| id | doaj.art-06cff5085adb43bb95c61d656a85c76e |
| institution | Directory Open Access Journal |
| issn | 2383-2754 |
| language | English |
| last_indexed | 2024-12-10T09:38:42Z |
| publishDate | 2022-07-01 |
| publisher | Islamic Azad University |
| record_format | Article |
| series | Journal of Research in Dental and Maxillofacial Sciences |
| spelling | doaj.art-06cff5085adb43bb95c61d656a85c76e2022-12-22T01:54:06ZengIslamic Azad UniversityJournal of Research in Dental and Maxillofacial Sciences2383-27542022-07-0173133141Minimum Inhibitory Concentration of Peganum Harmala Extract Against Candida SpeciesM Baharvand0M Hakemi-Vala1M Mehdipour2L Dehghani3E Jalali4F Anbari5 Department of Oral and Maxillofacial Medicine, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran Department of Microbiology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran Department of Oral and Maxillofacial Medicine, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran Private Dental Practice, Oral & Maxillofacial Radiology private practice, Miami, Florida, USA Department of Oral and Maxillofacial Medicine, School of Dentistry, Shahid Beheshti University of Medical Sciences, Tehran, Iran Background and Aim: There are some rich sources of antifungals in medicinal herbs. They have general acceptance due to their low cost and insignificant side effects. The efficacy of Peganum harmala (P. harmala) extract against Candida species was investigated in this study. Materials and Methods: In this in vitro study, we used the maceration technique to obtain P. harmala aqueous and alcoholic extracts, and assess their antifungal activity against different species of Candida albicans (C. albicans), Candida glabrata (C. glabrata) and Candida krusei (C. krusei). We used the microdilution method to calculate the minimum inhibitory concentration (MIC). The tests were repeated in triplicate for higher accuracy. Results: The aqueous and alcoholic P. harmala extracts showed antifungal activity against C. albicans in 100 mg/mL concentration. The antifungal efficacy of P. harmala alcoholic extract against C. glabrata (MIC: 100 mg/mL) was higher than the aqueous extract (MIC:200 mg/mL). The antifungal efficacy of P. harmala alcoholic extract against C. krusei (MIC: 1.56 mg/mL) was higher than the aqueous extract (MIC: 12.5 mg/mL). Conclusion: The aqueous and alcoholic extracts of P. harmala were effective against all species of Candida particularly Candida krusei. The alcoholic extract of P. harmala was more effective than its aqueous extract against C. glabrata and C. krusei.http://jrdms.dentaliau.ac.ir/article-1-348-en.htmlcandida albicanscandida glabratapeganum |
| spellingShingle | M Baharvand M Hakemi-Vala M Mehdipour L Dehghani E Jalali F Anbari Minimum Inhibitory Concentration of Peganum Harmala Extract Against Candida Species Journal of Research in Dental and Maxillofacial Sciences candida albicans candida glabrata peganum |
| title | Minimum Inhibitory Concentration of Peganum Harmala Extract Against Candida Species |
| title_full | Minimum Inhibitory Concentration of Peganum Harmala Extract Against Candida Species |
| title_fullStr | Minimum Inhibitory Concentration of Peganum Harmala Extract Against Candida Species |
| title_full_unstemmed | Minimum Inhibitory Concentration of Peganum Harmala Extract Against Candida Species |
| title_short | Minimum Inhibitory Concentration of Peganum Harmala Extract Against Candida Species |
| title_sort | minimum inhibitory concentration of peganum harmala extract against candida species |
| topic | candida albicans candida glabrata peganum |
| url | http://jrdms.dentaliau.ac.ir/article-1-348-en.html |
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