Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis
Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency lea...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2017-01-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124716317727 |
| _version_ | 1818265591527505920 |
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| author | Jun Ueda Akihito Harada Takashi Urahama Shinichi Machida Kazumitsu Maehara Masashi Hada Yoshinori Makino Jumpei Nogami Naoki Horikoshi Akihisa Osakabe Hiroyuki Taguchi Hiroki Tanaka Hiroaki Tachiwana Tatsuma Yao Minami Yamada Takashi Iwamoto Ayako Isotani Masahito Ikawa Taro Tachibana Yuki Okada Hiroshi Kimura Yasuyuki Ohkawa Hitoshi Kurumizaka Kazuo Yamagata |
| author_facet | Jun Ueda Akihito Harada Takashi Urahama Shinichi Machida Kazumitsu Maehara Masashi Hada Yoshinori Makino Jumpei Nogami Naoki Horikoshi Akihisa Osakabe Hiroyuki Taguchi Hiroki Tanaka Hiroaki Tachiwana Tatsuma Yao Minami Yamada Takashi Iwamoto Ayako Isotani Masahito Ikawa Taro Tachibana Yuki Okada Hiroshi Kimura Yasuyuki Ohkawa Hitoshi Kurumizaka Kazuo Yamagata |
| author_sort | Jun Ueda |
| collection | DOAJ |
| description | Cellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond. |
| first_indexed | 2024-12-12T19:53:15Z |
| format | Article |
| id | doaj.art-06d1e957ec7a424ab096f2a87b360b82 |
| institution | Directory Open Access Journal |
| issn | 2211-1247 |
| language | English |
| last_indexed | 2024-12-12T19:53:15Z |
| publishDate | 2017-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj.art-06d1e957ec7a424ab096f2a87b360b822022-12-22T00:13:56ZengElsevierCell Reports2211-12472017-01-0118359360010.1016/j.celrep.2016.12.065Testis-Specific Histone Variant H3t Gene Is Essential for Entry into SpermatogenesisJun Ueda0Akihito Harada1Takashi Urahama2Shinichi Machida3Kazumitsu Maehara4Masashi Hada5Yoshinori Makino6Jumpei Nogami7Naoki Horikoshi8Akihisa Osakabe9Hiroyuki Taguchi10Hiroki Tanaka11Hiroaki Tachiwana12Tatsuma Yao13Minami Yamada14Takashi Iwamoto15Ayako Isotani16Masahito Ikawa17Taro Tachibana18Yuki Okada19Hiroshi Kimura20Yasuyuki Ohkawa21Hitoshi Kurumizaka22Kazuo Yamagata23Center for Education in Laboratory Animal Research, Chubu University, Kasugai, Aichi 487-8501, JapanDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, JapanLaboratory of Structural Biology, Graduate School of Advanced Science & Engineering, Waseda University, Tokyo 162-8480, JapanLaboratory of Structural Biology, Graduate School of Advanced Science & Engineering, Waseda University, Tokyo 162-8480, JapanDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, JapanLaboratory of Pathology and Development, Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032, JapanLaboratory of Pathology and Development, Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032, JapanDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, JapanLaboratory of Structural Biology, Graduate School of Advanced Science & Engineering, Waseda University, Tokyo 162-8480, JapanLaboratory of Structural Biology, Graduate School of Advanced Science & Engineering, Waseda University, Tokyo 162-8480, JapanLaboratory of Structural Biology, Graduate School of Advanced Science & Engineering, Waseda University, Tokyo 162-8480, JapanLaboratory of Structural Biology, Graduate School of Advanced Science & Engineering, Waseda University, Tokyo 162-8480, JapanLaboratory of Structural Biology, Graduate School of Advanced Science & Engineering, Waseda University, Tokyo 162-8480, JapanResearch and Development Center, Fuso Pharmaceutical Industries, Ltd., Osaka 536-8523, JapanCenter for Education in Laboratory Animal Research, Chubu University, Kasugai, Aichi 487-8501, JapanCenter for Education in Laboratory Animal Research, Chubu University, Kasugai, Aichi 487-8501, JapanCenter for Genetic Analysis of Biological Responses, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita 565-0871, JapanCenter for Genetic Analysis of Biological Responses, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita 565-0871, JapanDepartment of Bioengineering, Graduate School of Engineering, Osaka City University, Osaka 558-8585, JapanLaboratory of Pathology and Development, Institute of Molecular and Cellular Biosciences, University of Tokyo, Tokyo 113-0032, JapanGraduate School of Bioscience and Biotechnology, Tokyo Institute of Technology, 4259 Nagatsuta-cho, Midori-ku, Yokohama 226-8501, JapanDivision of Transcriptomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka 812-8582, JapanLaboratory of Structural Biology, Graduate School of Advanced Science & Engineering, Waseda University, Tokyo 162-8480, JapanCenter for Genetic Analysis of Biological Responses, Research Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita 565-0871, JapanCellular differentiation is associated with dynamic chromatin remodeling in establishing a cell-type-specific epigenomic landscape. Here, we find that mouse testis-specific and replication-dependent histone H3 variant H3t is essential for very early stages of spermatogenesis. H3t gene deficiency leads to azoospermia because of the loss of haploid germ cells. When differentiating spermatogonia emerge in normal spermatogenesis, H3t appears and replaces the canonical H3 proteins. Structural and biochemical analyses reveal that H3t-containing nucleosomes are more flexible than the canonical nucleosomes. Thus, by incorporating H3t into the genome during spermatogonial differentiation, male germ cells are able to enter meiosis and beyond.http://www.sciencedirect.com/science/article/pii/S2211124716317727histone variantspermatogenesisnucleosomechromatincrystal structureepigeneticstestesmeiosisspermatogonial differentiationspermatozoa |
| spellingShingle | Jun Ueda Akihito Harada Takashi Urahama Shinichi Machida Kazumitsu Maehara Masashi Hada Yoshinori Makino Jumpei Nogami Naoki Horikoshi Akihisa Osakabe Hiroyuki Taguchi Hiroki Tanaka Hiroaki Tachiwana Tatsuma Yao Minami Yamada Takashi Iwamoto Ayako Isotani Masahito Ikawa Taro Tachibana Yuki Okada Hiroshi Kimura Yasuyuki Ohkawa Hitoshi Kurumizaka Kazuo Yamagata Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis Cell Reports histone variant spermatogenesis nucleosome chromatin crystal structure epigenetics testes meiosis spermatogonial differentiation spermatozoa |
| title | Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis |
| title_full | Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis |
| title_fullStr | Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis |
| title_full_unstemmed | Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis |
| title_short | Testis-Specific Histone Variant H3t Gene Is Essential for Entry into Spermatogenesis |
| title_sort | testis specific histone variant h3t gene is essential for entry into spermatogenesis |
| topic | histone variant spermatogenesis nucleosome chromatin crystal structure epigenetics testes meiosis spermatogonial differentiation spermatozoa |
| url | http://www.sciencedirect.com/science/article/pii/S2211124716317727 |
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