QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean
This study aimed to discover the quantitative trait loci (QTL) associated with a high seed protein content in soybean and unravel the potential candidate genes. We developed two recombinant inbred line populations: YS and SI, by crossing Saedanbaek (high protein) with YS2035-B-91-1-B-1 (low protein)...
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MDPI AG
2023-10-01
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| author | Hye Rang Park Jeong Hyun Seo Beom Kyu Kang Jun Hoi Kim Su Vin Heo Man Soo Choi Jee Yeon Ko Choon Song Kim |
| author_facet | Hye Rang Park Jeong Hyun Seo Beom Kyu Kang Jun Hoi Kim Su Vin Heo Man Soo Choi Jee Yeon Ko Choon Song Kim |
| author_sort | Hye Rang Park |
| collection | DOAJ |
| description | This study aimed to discover the quantitative trait loci (QTL) associated with a high seed protein content in soybean and unravel the potential candidate genes. We developed two recombinant inbred line populations: YS and SI, by crossing Saedanbaek (high protein) with YS2035-B-91-1-B-1 (low protein) and Saedanbaek with Ilmi (low protein), respectively, and evaluated the protein content for three consecutive years. Using single-nucleotide polymorphism (SNP)-marker-based linkage maps, four QTLs were located on chromosomes 15, 18, and 20 with high logarithm of odds values (5.9–55.0), contributing 5.5–66.0% phenotypic variance. In all three experimental years, <i>qPSD20-1</i> and <i>qPSD20-2</i> were stable and identified in overlapping positions in the YS and SI populations, respectively. Additionally, novel QTLs were identified on chromosomes 15 and 18. Considering the allelic sequence variation between parental lines, 28 annotated genes related to soybean seed protein—including starch, lipid, and fatty acid biosynthesis-related genes—were identified within the QTL regions. These genes could potentially affect protein accumulation during seed development, as well as sucrose and oil metabolism. Overall, this study offers insights into the genetic mechanisms underlying a high soybean protein content. The identified potential candidate genes can aid marker-assisted selection for developing soybean lines with an increased protein content. |
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| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T20:56:16Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-06d234f86d6b432d94ef5c45b4b23b9f2023-11-19T17:49:11ZengMDPI AGPlants2223-77472023-10-011220358910.3390/plants12203589QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of SoybeanHye Rang Park0Jeong Hyun Seo1Beom Kyu Kang2Jun Hoi Kim3Su Vin Heo4Man Soo Choi5Jee Yeon Ko6Choon Song Kim7Department of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of KoreaDepartment of Southern Area Crop Science, National Institute of Crop Science, Rural Development Administration, Miryang 50424, Republic of KoreaThis study aimed to discover the quantitative trait loci (QTL) associated with a high seed protein content in soybean and unravel the potential candidate genes. We developed two recombinant inbred line populations: YS and SI, by crossing Saedanbaek (high protein) with YS2035-B-91-1-B-1 (low protein) and Saedanbaek with Ilmi (low protein), respectively, and evaluated the protein content for three consecutive years. Using single-nucleotide polymorphism (SNP)-marker-based linkage maps, four QTLs were located on chromosomes 15, 18, and 20 with high logarithm of odds values (5.9–55.0), contributing 5.5–66.0% phenotypic variance. In all three experimental years, <i>qPSD20-1</i> and <i>qPSD20-2</i> were stable and identified in overlapping positions in the YS and SI populations, respectively. Additionally, novel QTLs were identified on chromosomes 15 and 18. Considering the allelic sequence variation between parental lines, 28 annotated genes related to soybean seed protein—including starch, lipid, and fatty acid biosynthesis-related genes—were identified within the QTL regions. These genes could potentially affect protein accumulation during seed development, as well as sucrose and oil metabolism. Overall, this study offers insights into the genetic mechanisms underlying a high soybean protein content. The identified potential candidate genes can aid marker-assisted selection for developing soybean lines with an increased protein content.https://www.mdpi.com/2223-7747/12/20/3589quantitative trait locisoybean proteinhigh proteingenetic mapsingle-nucleotide polymorphism |
| spellingShingle | Hye Rang Park Jeong Hyun Seo Beom Kyu Kang Jun Hoi Kim Su Vin Heo Man Soo Choi Jee Yeon Ko Choon Song Kim QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean Plants quantitative trait loci soybean protein high protein genetic map single-nucleotide polymorphism |
| title | QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean |
| title_full | QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean |
| title_fullStr | QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean |
| title_full_unstemmed | QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean |
| title_short | QTLs and Candidate Genes for Seed Protein Content in Two Recombinant Inbred Line Populations of Soybean |
| title_sort | qtls and candidate genes for seed protein content in two recombinant inbred line populations of soybean |
| topic | quantitative trait loci soybean protein high protein genetic map single-nucleotide polymorphism |
| url | https://www.mdpi.com/2223-7747/12/20/3589 |
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