Massively-multiplexed epitope mapping techniques for viral antigen discovery
Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-09-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1192385/full |
| _version_ | 1797673965776273408 |
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| author | Diya Hu Aaron T. Irving Aaron T. Irving Aaron T. Irving Aaron T. Irving |
| author_facet | Diya Hu Aaron T. Irving Aaron T. Irving Aaron T. Irving Aaron T. Irving |
| author_sort | Diya Hu |
| collection | DOAJ |
| description | Following viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery. |
| first_indexed | 2024-03-11T21:53:16Z |
| format | Article |
| id | doaj.art-06d337124b2b4998ae6e8ffa6f44cb33 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-03-11T21:53:16Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-06d337124b2b4998ae6e8ffa6f44cb332023-09-26T05:58:11ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-09-011410.3389/fimmu.2023.11923851192385Massively-multiplexed epitope mapping techniques for viral antigen discoveryDiya Hu0Aaron T. Irving1Aaron T. Irving2Aaron T. Irving3Aaron T. Irving4Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University, Haining, ChinaDepartment of Clinical Laboratory Studies, Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, ChinaCentre for Infection, Immunity & Cancer, Zhejiang University-University of Edinburgh Institute, Zhejiang University School of Medicine, Zhejiang University, Haining, ChinaBiomedical and Health Translational Research Centre of Zhejiang Province (BIMET), Haining, ChinaCollege of Medicine & Veterinary Medicine, The University of Edinburgh, Edinburgh, United KingdomFollowing viral infection, viral antigens bind specifically to receptors on the surface of lymphocytes thereby activating adaptive immunity in the host. An epitope, the smallest structural and functional unit of an antigen, binds specifically to an antibody or antigen receptor, to serve as key sites for the activation of adaptive immunity. The complexity and diverse range of epitopes are essential to study and map for the diagnosis of disease, the design of vaccines and for immunotherapy. Mapping the location of these specific epitopes has become a hot topic in immunology and immune therapy. Recently, epitope mapping techniques have evolved to become multiplexed, with the advent of high-throughput sequencing and techniques such as bacteriophage-display libraries and deep mutational scanning. Here, we briefly introduce the principles, advantages, and disadvantages of the latest epitope mapping techniques with examples for viral antigen discovery.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1192385/fullepitopeepitope mappingphage-displayT cellB cell |
| spellingShingle | Diya Hu Aaron T. Irving Aaron T. Irving Aaron T. Irving Aaron T. Irving Massively-multiplexed epitope mapping techniques for viral antigen discovery Frontiers in Immunology epitope epitope mapping phage-display T cell B cell |
| title | Massively-multiplexed epitope mapping techniques for viral antigen discovery |
| title_full | Massively-multiplexed epitope mapping techniques for viral antigen discovery |
| title_fullStr | Massively-multiplexed epitope mapping techniques for viral antigen discovery |
| title_full_unstemmed | Massively-multiplexed epitope mapping techniques for viral antigen discovery |
| title_short | Massively-multiplexed epitope mapping techniques for viral antigen discovery |
| title_sort | massively multiplexed epitope mapping techniques for viral antigen discovery |
| topic | epitope epitope mapping phage-display T cell B cell |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1192385/full |
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