Contamination inside CT gantry in the SARS-CoV-2 era
Abstract We investigated whether the internal gantry components of our computed tomography (CT) scanner contain severe acute respiratory syndrome 2 (SARS-CoV-2) ribonucleic acid (RNA), bacterial or fungal agents. From 1 to 27 March 2020, we performed 180 examinations of patients with confirmed SARS-...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-10-01
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| Series: | European Radiology Experimental |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s41747-020-00182-1 |
| _version_ | 1818243861431975936 |
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| author | João Matos Francesco Paparo Marco Mori Alessio Veneziano Marina Sartini Maria Luisa Cristina Gian Andrea Rollandi |
| author_facet | João Matos Francesco Paparo Marco Mori Alessio Veneziano Marina Sartini Maria Luisa Cristina Gian Andrea Rollandi |
| author_sort | João Matos |
| collection | DOAJ |
| description | Abstract We investigated whether the internal gantry components of our computed tomography (CT) scanner contain severe acute respiratory syndrome 2 (SARS-CoV-2) ribonucleic acid (RNA), bacterial or fungal agents. From 1 to 27 March 2020, we performed 180 examinations of patients with confirmed SARS-CoV-2 infection using a dedicated CT scanner. On 27 March 2020, this CT gantry was opened and sampled in each of the following components: (a) gantry case; (b) inward airflow filter; (c) gantry motor; (d) x-ray tube; (e) outflow fan; (f) fan grid; (g) detectors; and (h) x-ray tube filter. To detect SARS-CoV-2 RNA, samples were analysed using reverse transcriptase-polymerase chain reaction (RT-PCR). To detect bacterial or fungal agents, samples have been collected using “replicate organism detection and counting” contact plates of 24 cm2, containing tryptic soy agar, and subsequently cultured. RT-PCR detected SARS-CoV-2 RNA in the inward airflow filter sample. RT-PCR of remaining gantry samples did not reveal the presence of SARS-CoV-2 RNA. Neither bacterial nor fungal agents grew in the agar-based growth medium after the incubation period. Our data showed that SARS-Cov-2 RNA can be found inside the CT gantry only in the inward airflow filter. All remaining CT gantry components were devoid of SARS-CoV-2 RNA. |
| first_indexed | 2024-12-12T14:07:51Z |
| format | Article |
| id | doaj.art-06d3a191808d4c7eacc87fb5c8b928bf |
| institution | Directory Open Access Journal |
| issn | 2509-9280 |
| language | English |
| last_indexed | 2024-12-12T14:07:51Z |
| publishDate | 2020-10-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | European Radiology Experimental |
| spelling | doaj.art-06d3a191808d4c7eacc87fb5c8b928bf2022-12-22T00:22:09ZengSpringerOpenEuropean Radiology Experimental2509-92802020-10-01411410.1186/s41747-020-00182-1Contamination inside CT gantry in the SARS-CoV-2 eraJoão Matos0Francesco Paparo1Marco Mori2Alessio Veneziano3Marina Sartini4Maria Luisa Cristina5Gian Andrea Rollandi6DISSAL–Department of Health Sciences, University of GenoaS.C. Radiodiagnostica, EO Ospedali GallieraS.C. Laboratorio Analisi, EO Ospedali GallieraIndependent ResearcherS.S.D. U.O. a Direzione Universitaria Igiene Ospedaliera, EO Ospedali GallieraS.S.D. U.O. a Direzione Universitaria Igiene Ospedaliera, EO Ospedali GallieraS.C. Radiodiagnostica, EO Ospedali GallieraAbstract We investigated whether the internal gantry components of our computed tomography (CT) scanner contain severe acute respiratory syndrome 2 (SARS-CoV-2) ribonucleic acid (RNA), bacterial or fungal agents. From 1 to 27 March 2020, we performed 180 examinations of patients with confirmed SARS-CoV-2 infection using a dedicated CT scanner. On 27 March 2020, this CT gantry was opened and sampled in each of the following components: (a) gantry case; (b) inward airflow filter; (c) gantry motor; (d) x-ray tube; (e) outflow fan; (f) fan grid; (g) detectors; and (h) x-ray tube filter. To detect SARS-CoV-2 RNA, samples were analysed using reverse transcriptase-polymerase chain reaction (RT-PCR). To detect bacterial or fungal agents, samples have been collected using “replicate organism detection and counting” contact plates of 24 cm2, containing tryptic soy agar, and subsequently cultured. RT-PCR detected SARS-CoV-2 RNA in the inward airflow filter sample. RT-PCR of remaining gantry samples did not reveal the presence of SARS-CoV-2 RNA. Neither bacterial nor fungal agents grew in the agar-based growth medium after the incubation period. Our data showed that SARS-Cov-2 RNA can be found inside the CT gantry only in the inward airflow filter. All remaining CT gantry components were devoid of SARS-CoV-2 RNA.http://link.springer.com/article/10.1186/s41747-020-00182-1COVID-19Patient safetySafety managementSevere acute respiratory syndrome coronavirus 2Tomography scanners (x-ray computed) |
| spellingShingle | João Matos Francesco Paparo Marco Mori Alessio Veneziano Marina Sartini Maria Luisa Cristina Gian Andrea Rollandi Contamination inside CT gantry in the SARS-CoV-2 era European Radiology Experimental COVID-19 Patient safety Safety management Severe acute respiratory syndrome coronavirus 2 Tomography scanners (x-ray computed) |
| title | Contamination inside CT gantry in the SARS-CoV-2 era |
| title_full | Contamination inside CT gantry in the SARS-CoV-2 era |
| title_fullStr | Contamination inside CT gantry in the SARS-CoV-2 era |
| title_full_unstemmed | Contamination inside CT gantry in the SARS-CoV-2 era |
| title_short | Contamination inside CT gantry in the SARS-CoV-2 era |
| title_sort | contamination inside ct gantry in the sars cov 2 era |
| topic | COVID-19 Patient safety Safety management Severe acute respiratory syndrome coronavirus 2 Tomography scanners (x-ray computed) |
| url | http://link.springer.com/article/10.1186/s41747-020-00182-1 |
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