Changes of Contents of Five Phenolic Acids Ingredients in Phyllanthus emblica L. during Different Harvesting Times by HPLC
Objective: To establish the method for content determination of the five phenolic acids ingredients of Phyllanthus emblica L. in different harvest time, and to investigate its variation rules so as to determine the best harvest time. Methods: The contents of Epigallocatechin, Corilagin, Scorpion Bip...
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2023-08-01
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| Series: | Shipin gongye ke-ji |
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| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100021 |
| _version_ | 1827861220378542080 |
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| author | Chunlan YANG Wanting MAI Shengjie CHEN Yong YE Jie WANG |
| author_facet | Chunlan YANG Wanting MAI Shengjie CHEN Yong YE Jie WANG |
| author_sort | Chunlan YANG |
| collection | DOAJ |
| description | Objective: To establish the method for content determination of the five phenolic acids ingredients of Phyllanthus emblica L. in different harvest time, and to investigate its variation rules so as to determine the best harvest time. Methods: The contents of Epigallocatechin, Corilagin, Scorpion Biphenyl acid, Ellagic acid and Scorpion acid of Phyllanthus emblica L. in different harvest time were determined by HPLC, and the total amount of accumulation of the five active ingredients was calculated. The chromatographic column was Inertsil ODS-3 C18, acetonitrile−0.1% phosphoric acid aqueous solution as mobile phase for gradient elution. A diode array detector was used with full-wavelength scanning. Flow rate was 1.0 mL·min−1. Injection was 10 μL. Used SPSS 24.0 software for principal component analysis, three principal components were selected and the comprehensive score was calculated. Results: The above five ingredients had a good linear relationship, and the correlation coefficients was more than 0.998, while the average recovery rates were 98.30%~101.22% and RSD were 0.43%~2.66%. There were some differences in the contents of the five phenolic acids ingredients in the leaves of Phyllanthus emblica L. during different harvesting periods. The contents of above five ingredients were 0.2317~1.5074, 1.9517~4.6593, 2.5105~6.3325, 2.2440~5.3386, 13.5570~39.1897 mg·g−1, respectively. The change in content of the five phenolic acids ingredients in different harvest time was: July>May>August>June>January>September>April>February>October>December>March>November. The total content was the highest in July, which was (55.158±0.83) mg/g and showed a dynamic trend of decreasing first and then increasing. Principal component analysis results showed that the month with the highest comprehensive score was July. Conclusion: Established method can be used to determine the contents of the five phenolic acids ingredients of Phyllanthus emblica L. in different harvest time. July was the best harvest time for Phyllanthus emblica L. |
| first_indexed | 2024-03-12T13:34:58Z |
| format | Article |
| id | doaj.art-06e2f238646a41b58734852befa230ff |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-03-12T13:34:58Z |
| publishDate | 2023-08-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
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| series | Shipin gongye ke-ji |
| spelling | doaj.art-06e2f238646a41b58734852befa230ff2023-08-24T06:00:17ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062023-08-01441534635310.13386/j.issn1002-0306.20221000212022100021-15Changes of Contents of Five Phenolic Acids Ingredients in Phyllanthus emblica L. during Different Harvesting Times by HPLCChunlan YANG0Wanting MAI1Shengjie CHEN2Yong YE3Jie WANG4School of Pharmacy, Guangxi Medical University, Nanning 530021, ChinaSchool of Pharmacy, Guangxi Medical University, Nanning 530021, ChinaSchool of Pharmacy, Guangxi Medical University, Nanning 530021, ChinaSchool of Pharmacy, Guangxi Medical University, Nanning 530021, ChinaSchool of Pharmacy, Guangxi Medical University, Nanning 530021, ChinaObjective: To establish the method for content determination of the five phenolic acids ingredients of Phyllanthus emblica L. in different harvest time, and to investigate its variation rules so as to determine the best harvest time. Methods: The contents of Epigallocatechin, Corilagin, Scorpion Biphenyl acid, Ellagic acid and Scorpion acid of Phyllanthus emblica L. in different harvest time were determined by HPLC, and the total amount of accumulation of the five active ingredients was calculated. The chromatographic column was Inertsil ODS-3 C18, acetonitrile−0.1% phosphoric acid aqueous solution as mobile phase for gradient elution. A diode array detector was used with full-wavelength scanning. Flow rate was 1.0 mL·min−1. Injection was 10 μL. Used SPSS 24.0 software for principal component analysis, three principal components were selected and the comprehensive score was calculated. Results: The above five ingredients had a good linear relationship, and the correlation coefficients was more than 0.998, while the average recovery rates were 98.30%~101.22% and RSD were 0.43%~2.66%. There were some differences in the contents of the five phenolic acids ingredients in the leaves of Phyllanthus emblica L. during different harvesting periods. The contents of above five ingredients were 0.2317~1.5074, 1.9517~4.6593, 2.5105~6.3325, 2.2440~5.3386, 13.5570~39.1897 mg·g−1, respectively. The change in content of the five phenolic acids ingredients in different harvest time was: July>May>August>June>January>September>April>February>October>December>March>November. The total content was the highest in July, which was (55.158±0.83) mg/g and showed a dynamic trend of decreasing first and then increasing. Principal component analysis results showed that the month with the highest comprehensive score was July. Conclusion: Established method can be used to determine the contents of the five phenolic acids ingredients of Phyllanthus emblica L. in different harvest time. July was the best harvest time for Phyllanthus emblica L.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100021phyllanthus emblica l.harvesting periodhigh performance liquid chromatography (hplc)content determinationvariation rule |
| spellingShingle | Chunlan YANG Wanting MAI Shengjie CHEN Yong YE Jie WANG Changes of Contents of Five Phenolic Acids Ingredients in Phyllanthus emblica L. during Different Harvesting Times by HPLC Shipin gongye ke-ji phyllanthus emblica l. harvesting period high performance liquid chromatography (hplc) content determination variation rule |
| title | Changes of Contents of Five Phenolic Acids Ingredients in Phyllanthus emblica L. during Different Harvesting Times by HPLC |
| title_full | Changes of Contents of Five Phenolic Acids Ingredients in Phyllanthus emblica L. during Different Harvesting Times by HPLC |
| title_fullStr | Changes of Contents of Five Phenolic Acids Ingredients in Phyllanthus emblica L. during Different Harvesting Times by HPLC |
| title_full_unstemmed | Changes of Contents of Five Phenolic Acids Ingredients in Phyllanthus emblica L. during Different Harvesting Times by HPLC |
| title_short | Changes of Contents of Five Phenolic Acids Ingredients in Phyllanthus emblica L. during Different Harvesting Times by HPLC |
| title_sort | changes of contents of five phenolic acids ingredients in phyllanthus emblica l during different harvesting times by hplc |
| topic | phyllanthus emblica l. harvesting period high performance liquid chromatography (hplc) content determination variation rule |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2022100021 |
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