The importance of intermediate filaments in the shape maintenance of myoblast model tissues
Liquid and elastic behaviours of tissues drive their morphology and response to the environment. They appear as the first insight into tissue mechanics. We explore the role of individual cell properties on spheroids of mouse muscle precursor cells and investigate the role of intermediate filaments o...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2022-12-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/76409 |
| _version_ | 1828266534214041600 |
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| author | Irène Nagle Florence Delort Sylvie Hénon Claire Wilhelm Sabrina Batonnet-Pichon Myriam Reffay |
| author_facet | Irène Nagle Florence Delort Sylvie Hénon Claire Wilhelm Sabrina Batonnet-Pichon Myriam Reffay |
| author_sort | Irène Nagle |
| collection | DOAJ |
| description | Liquid and elastic behaviours of tissues drive their morphology and response to the environment. They appear as the first insight into tissue mechanics. We explore the role of individual cell properties on spheroids of mouse muscle precursor cells and investigate the role of intermediate filaments on surface tension and Young’s modulus. By flattening multicellular myoblast aggregates under magnetic constraint, we measure their rigidity and surface tension and show that they act as highly sensitive macroscopic reporters closely related to microscopic local tension and effective adhesion. Shedding light on the major contributions of acto-myosin contractility, actin organization, and intercellular adhesions, we reveal the role of a major component of intermediate filaments in the muscle, desmin and its organization, on the macroscopic mechanics of these tissue models. Implicated in the mechanical and shape integrity of cells, intermediate filaments are found to be crucial to the mechanics of unorganized muscle tissue models even at an early stage of differentiation both in terms of elasticity and surface tension. |
| first_indexed | 2024-04-13T04:53:59Z |
| format | Article |
| id | doaj.art-06e70ba60be3489bace48b304fbf7cec |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-13T04:53:59Z |
| publishDate | 2022-12-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-06e70ba60be3489bace48b304fbf7cec2022-12-22T03:01:34ZengeLife Sciences Publications LtdeLife2050-084X2022-12-011110.7554/eLife.76409The importance of intermediate filaments in the shape maintenance of myoblast model tissuesIrène Nagle0Florence Delort1Sylvie Hénon2Claire Wilhelm3Sabrina Batonnet-Pichon4Myriam Reffay5https://orcid.org/0000-0002-3695-2789Laboratoire Matière et Systèmes Complexes, UMR 7057, Université Paris Cité and CNRS, Paris, FranceLaboratoire Biologie Fonctionnelle et Adaptative, UMR 8251, Université Paris Cité and CNRS, Paris, FranceLaboratoire Matière et Systèmes Complexes, UMR 7057, Université Paris Cité and CNRS, Paris, FranceLaboratoire Matière et Systèmes Complexes, UMR 7057, Université Paris Cité and CNRS, Paris, FranceLaboratoire Biologie Fonctionnelle et Adaptative, UMR 8251, Université Paris Cité and CNRS, Paris, FranceLaboratoire Matière et Systèmes Complexes, UMR 7057, Université Paris Cité and CNRS, Paris, FranceLiquid and elastic behaviours of tissues drive their morphology and response to the environment. They appear as the first insight into tissue mechanics. We explore the role of individual cell properties on spheroids of mouse muscle precursor cells and investigate the role of intermediate filaments on surface tension and Young’s modulus. By flattening multicellular myoblast aggregates under magnetic constraint, we measure their rigidity and surface tension and show that they act as highly sensitive macroscopic reporters closely related to microscopic local tension and effective adhesion. Shedding light on the major contributions of acto-myosin contractility, actin organization, and intercellular adhesions, we reveal the role of a major component of intermediate filaments in the muscle, desmin and its organization, on the macroscopic mechanics of these tissue models. Implicated in the mechanical and shape integrity of cells, intermediate filaments are found to be crucial to the mechanics of unorganized muscle tissue models even at an early stage of differentiation both in terms of elasticity and surface tension.https://elifesciences.org/articles/76409mechanobiologysurface tensionelasticitymagnetic nanoparticlesintermediate filamentmyoblasts |
| spellingShingle | Irène Nagle Florence Delort Sylvie Hénon Claire Wilhelm Sabrina Batonnet-Pichon Myriam Reffay The importance of intermediate filaments in the shape maintenance of myoblast model tissues eLife mechanobiology surface tension elasticity magnetic nanoparticles intermediate filament myoblasts |
| title | The importance of intermediate filaments in the shape maintenance of myoblast model tissues |
| title_full | The importance of intermediate filaments in the shape maintenance of myoblast model tissues |
| title_fullStr | The importance of intermediate filaments in the shape maintenance of myoblast model tissues |
| title_full_unstemmed | The importance of intermediate filaments in the shape maintenance of myoblast model tissues |
| title_short | The importance of intermediate filaments in the shape maintenance of myoblast model tissues |
| title_sort | importance of intermediate filaments in the shape maintenance of myoblast model tissues |
| topic | mechanobiology surface tension elasticity magnetic nanoparticles intermediate filament myoblasts |
| url | https://elifesciences.org/articles/76409 |
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