Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

Optimizing CRISPR/Cas9 for the Diatom Phaeodactylum tricornutum

CRISPR/Cas9 is a powerful tool for genome editing. We constructed an easy-to-handle expression vector for application in the model organism Phaeodactylum tricornutum and tested its capabilities in order to apply CRISPR/Cas9 technology for our purpose. In our experiments, we targeted two different ge...

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Bibliographic Details
Main Authors: Daniel Stukenberg, Stefan Zauner, Gianluca Dell’Aquila, Uwe G. Maier
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-06-01
Series:Frontiers in Plant Science
Subjects:
CRISPR-Cas
genome editing
Phaeodactylum tricurnutum
diatom
off-target
Online Access:https://www.frontiersin.org/article/10.3389/fpls.2018.00740/full

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