Integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in Artemisia argyi
Artemisia argyi (A. argyi) is a medicinal plant belonging to the Asteraceae family and Artemisia genus. Flavonoids abundant in A. argyi are associated with anti-inflammatory, anticancer, and antioxidative effects. Eupatilin and jaceosidin are representative polymethoxy flavonoids with medicinal prop...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1186023/full |
| _version_ | 1797839984809476096 |
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| author | Suhyeon Lee Hyo Jun Won Hyo Jun Won Seunghyun Ban Yun Ji Park Sang Min Kim Sang Min Kim Hyoung Seok Kim Jaeyoung Choi Ho-Youn Kim Ho-Youn Kim Jae Hoon Lee Jae Hoon Lee Je Hyeong Jung |
| author_facet | Suhyeon Lee Hyo Jun Won Hyo Jun Won Seunghyun Ban Yun Ji Park Sang Min Kim Sang Min Kim Hyoung Seok Kim Jaeyoung Choi Ho-Youn Kim Ho-Youn Kim Jae Hoon Lee Jae Hoon Lee Je Hyeong Jung |
| author_sort | Suhyeon Lee |
| collection | DOAJ |
| description | Artemisia argyi (A. argyi) is a medicinal plant belonging to the Asteraceae family and Artemisia genus. Flavonoids abundant in A. argyi are associated with anti-inflammatory, anticancer, and antioxidative effects. Eupatilin and jaceosidin are representative polymethoxy flavonoids with medicinal properties significant enough to warrant the development of drugs using their components. However, the biosynthetic pathways and related genes of these compounds have not been fully explored in A. argyi. This study comprehensively analyzed the transcriptome data and flavonoids contents from four different tissues of A. argyi (young leaves, old leaves, trichomes collected from stems, and stems without trichomes) for the first time. We obtained 41,398 unigenes through the de-novo assembly of transcriptome data and mined promising candidate genes involved in the biosynthesis of eupatilin and jaceosidin using differentially expressed genes, hierarchical clustering, phylogenetic tree, and weighted gene co-expression analysis. Our analysis led to the identification of a total of 7,265 DEGs, among which 153 genes were annotated as flavonoid-related genes. In particular, we were able to identify eight putative flavone-6-hydroxylase (F6H) genes, which were responsible for providing a methyl group acceptor into flavone basic skeleton. Furthermore, five O-methyltransferases (OMTs) gene were identified, which were required for the site-specific O-methylation during the biosynthesis of eupatilin and jaceosidin. Although further validation would be necessary, our findings pave the way for the modification and mass-production of pharmacologically important polymethoxy flavonoids through genetic engineering and synthetic biological approaches. |
| first_indexed | 2024-04-09T16:06:58Z |
| format | Article |
| id | doaj.art-06e9e78ed6ca46e18b4046ae4191b4b0 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-09T16:06:58Z |
| publishDate | 2023-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-06e9e78ed6ca46e18b4046ae4191b4b02023-04-25T04:51:29ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2023-04-011410.3389/fpls.2023.11860231186023Integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in Artemisia argyiSuhyeon Lee0Hyo Jun Won1Hyo Jun Won2Seunghyun Ban3Yun Ji Park4Sang Min Kim5Sang Min Kim6Hyoung Seok Kim7Jaeyoung Choi8Ho-Youn Kim9Ho-Youn Kim10Jae Hoon Lee11Jae Hoon Lee12Je Hyeong Jung13Smart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon, Republic of KoreaDivision of Bio-Medical Science & Technology, KIST School, University of Science and Technology (UST), Daejeon, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon, Republic of KoreaDivision of Bio-Medical Science & Technology, KIST School, University of Science and Technology (UST), Daejeon, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon, Republic of KoreaDepartment of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon, Republic of KoreaDivision of Bio-Medical Science & Technology, KIST School, University of Science and Technology (UST), Daejeon, Republic of KoreaDepartment of Agricultural Biotechnology, Seoul National University, Seoul, Republic of KoreaResearch Institute of Agriculture and Life Sciences, Seoul National University, Seoul, Republic of KoreaSmart Farm Research Center, Korea Institute of Science and Technology (KIST), Gangneung, Gangwon, Republic of KoreaArtemisia argyi (A. argyi) is a medicinal plant belonging to the Asteraceae family and Artemisia genus. Flavonoids abundant in A. argyi are associated with anti-inflammatory, anticancer, and antioxidative effects. Eupatilin and jaceosidin are representative polymethoxy flavonoids with medicinal properties significant enough to warrant the development of drugs using their components. However, the biosynthetic pathways and related genes of these compounds have not been fully explored in A. argyi. This study comprehensively analyzed the transcriptome data and flavonoids contents from four different tissues of A. argyi (young leaves, old leaves, trichomes collected from stems, and stems without trichomes) for the first time. We obtained 41,398 unigenes through the de-novo assembly of transcriptome data and mined promising candidate genes involved in the biosynthesis of eupatilin and jaceosidin using differentially expressed genes, hierarchical clustering, phylogenetic tree, and weighted gene co-expression analysis. Our analysis led to the identification of a total of 7,265 DEGs, among which 153 genes were annotated as flavonoid-related genes. In particular, we were able to identify eight putative flavone-6-hydroxylase (F6H) genes, which were responsible for providing a methyl group acceptor into flavone basic skeleton. Furthermore, five O-methyltransferases (OMTs) gene were identified, which were required for the site-specific O-methylation during the biosynthesis of eupatilin and jaceosidin. Although further validation would be necessary, our findings pave the way for the modification and mass-production of pharmacologically important polymethoxy flavonoids through genetic engineering and synthetic biological approaches.https://www.frontiersin.org/articles/10.3389/fpls.2023.1186023/fullArtemisia argyiflavonoideupatilinjaceosidintranscriptomebiosynthetic pathway |
| spellingShingle | Suhyeon Lee Hyo Jun Won Hyo Jun Won Seunghyun Ban Yun Ji Park Sang Min Kim Sang Min Kim Hyoung Seok Kim Jaeyoung Choi Ho-Youn Kim Ho-Youn Kim Jae Hoon Lee Jae Hoon Lee Je Hyeong Jung Integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in Artemisia argyi Frontiers in Plant Science Artemisia argyi flavonoid eupatilin jaceosidin transcriptome biosynthetic pathway |
| title | Integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in Artemisia argyi |
| title_full | Integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in Artemisia argyi |
| title_fullStr | Integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in Artemisia argyi |
| title_full_unstemmed | Integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in Artemisia argyi |
| title_short | Integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in Artemisia argyi |
| title_sort | integrative analysis of metabolite and transcriptome reveals biosynthetic pathway and candidate genes for eupatilin and jaceosidin biosynthesis in artemisia argyi |
| topic | Artemisia argyi flavonoid eupatilin jaceosidin transcriptome biosynthetic pathway |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1186023/full |
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