Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid Reactions
Min-Quan Hui,1,2,* Yan-Ni Mi,1,* Yu-Fan Ma,2 Tao Chen,2 Yong-Xiao Cao1 1Department of Pharmacology, Health Science Center, Xi’an Jiaotong University, Xi’an, People’s Republic of China; 2Xi’an Libang Pharmaceutical, Xi’an, People’s Republic...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2021-05-01
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| Series: | International Journal of Nanomedicine |
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| Online Access: | https://www.dovepress.com/preparation-and-evaluation-of-lipid-emulsion-containing-13-vitamins-fo-peer-reviewed-fulltext-article-IJN |
| _version_ | 1818344613929287680 |
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| author | Hui MQ Mi YN Ma YF Chen T Cao YX |
| author_facet | Hui MQ Mi YN Ma YF Chen T Cao YX |
| author_sort | Hui MQ |
| collection | DOAJ |
| description | Min-Quan Hui,1,2,* Yan-Ni Mi,1,* Yu-Fan Ma,2 Tao Chen,2 Yong-Xiao Cao1 1Department of Pharmacology, Health Science Center, Xi’an Jiaotong University, Xi’an, People’s Republic of China; 2Xi’an Libang Pharmaceutical, Xi’an, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yong-Xiao CaoDepartment of Pharmacology, Health Science Center, Xi’an Jiaotong University, 76 Yanta West Road, Xi’an, 710061, People’s Republic of ChinaTel +86-29-8265-5140Email yxy@xjtu.edu.cnObjective: Multivitamins containing Tween 80 can cause anaphylactoid reactions. The objective of this study was to develop a new lipid emulsion containing 13 fat- and water-soluble vitamins for injection (13V-LE) that were simultaneously dissolved in one bottle and to evaluate the stability of and anaphylactoid reactions to 13V-LE.Methods: Particle size, ζ-potential, and polydispersity of 13V-LE were assayed with a Zetasizer Nano ZS. Entrapment efficiency of 13V-LE was determined with HPLC. Behavior, histamine, and blood pressure of beagle dogs were investigated by observation, fluorospectrophotometry, and sphygmomanometry.Results: The 13V-LE with the smallest particles and highest entrapment efficiency with stable ζ-potential was composed of soybean oil, glycerin (2.25%, w:v), egg lecithin (1.2%, w:v), and purified water. There was no obvious change in characteristics of the 13V-LE samples in terms of appearance, size distribution, ζ-potential, pH value, or concentration over 6 months. In anaphylactoid reactions tests, when being administered with the multivitamin Infuvite Adult containing Tween 80, six beagles showed grade IV symptoms (P< 0.01 vs control), low blood pressure, and high plasma-histamine concentrations (P< 0.05 or P< 0.01). However, there were no significant differences in behavior, blood pressure, or histamine concentration in the dogs before and after administration in the 13V-LE group.Conclusion: The 13V-LE formulation is a suitable intravenous lipid emulsion without anaphylactoid reactions.Keywords: anaphylactoid reaction, multivitamins, lipid emulsion, beagle dog |
| first_indexed | 2024-12-13T16:49:16Z |
| format | Article |
| id | doaj.art-06ea2486e2224e5d8dbe3ed1dd28d4ff |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-13T16:49:16Z |
| publishDate | 2021-05-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-06ea2486e2224e5d8dbe3ed1dd28d4ff2022-12-21T23:38:04ZengDove Medical PressInternational Journal of Nanomedicine1178-20132021-05-01Volume 163317332764724Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid ReactionsHui MQMi YNMa YFChen TCao YXMin-Quan Hui,1,2,* Yan-Ni Mi,1,* Yu-Fan Ma,2 Tao Chen,2 Yong-Xiao Cao1 1Department of Pharmacology, Health Science Center, Xi’an Jiaotong University, Xi’an, People’s Republic of China; 2Xi’an Libang Pharmaceutical, Xi’an, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yong-Xiao CaoDepartment of Pharmacology, Health Science Center, Xi’an Jiaotong University, 76 Yanta West Road, Xi’an, 710061, People’s Republic of ChinaTel +86-29-8265-5140Email yxy@xjtu.edu.cnObjective: Multivitamins containing Tween 80 can cause anaphylactoid reactions. The objective of this study was to develop a new lipid emulsion containing 13 fat- and water-soluble vitamins for injection (13V-LE) that were simultaneously dissolved in one bottle and to evaluate the stability of and anaphylactoid reactions to 13V-LE.Methods: Particle size, ζ-potential, and polydispersity of 13V-LE were assayed with a Zetasizer Nano ZS. Entrapment efficiency of 13V-LE was determined with HPLC. Behavior, histamine, and blood pressure of beagle dogs were investigated by observation, fluorospectrophotometry, and sphygmomanometry.Results: The 13V-LE with the smallest particles and highest entrapment efficiency with stable ζ-potential was composed of soybean oil, glycerin (2.25%, w:v), egg lecithin (1.2%, w:v), and purified water. There was no obvious change in characteristics of the 13V-LE samples in terms of appearance, size distribution, ζ-potential, pH value, or concentration over 6 months. In anaphylactoid reactions tests, when being administered with the multivitamin Infuvite Adult containing Tween 80, six beagles showed grade IV symptoms (P< 0.01 vs control), low blood pressure, and high plasma-histamine concentrations (P< 0.05 or P< 0.01). However, there were no significant differences in behavior, blood pressure, or histamine concentration in the dogs before and after administration in the 13V-LE group.Conclusion: The 13V-LE formulation is a suitable intravenous lipid emulsion without anaphylactoid reactions.Keywords: anaphylactoid reaction, multivitamins, lipid emulsion, beagle doghttps://www.dovepress.com/preparation-and-evaluation-of-lipid-emulsion-containing-13-vitamins-fo-peer-reviewed-fulltext-article-IJNanaphylactoid reactionformulationmultivitaminsintravenous lipid emulsionstability |
| spellingShingle | Hui MQ Mi YN Ma YF Chen T Cao YX Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid Reactions International Journal of Nanomedicine anaphylactoid reaction formulation multivitamins intravenous lipid emulsion stability |
| title | Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid Reactions |
| title_full | Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid Reactions |
| title_fullStr | Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid Reactions |
| title_full_unstemmed | Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid Reactions |
| title_short | Preparation and Evaluation of Lipid Emulsion Containing 13 Vitamins for Injection Without Anaphylactoid Reactions |
| title_sort | preparation and evaluation of lipid emulsion containing 13 vitamins for injection without anaphylactoid reactions |
| topic | anaphylactoid reaction formulation multivitamins intravenous lipid emulsion stability |
| url | https://www.dovepress.com/preparation-and-evaluation-of-lipid-emulsion-containing-13-vitamins-fo-peer-reviewed-fulltext-article-IJN |
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