<i>BaZFP1,</i> a C2H2 Subfamily Gene in Desiccation-Tolerant Moss <i>Bryum argenteum</i>, Positively Regulates Growth and Development in <i>Arabidopsis</i> and Mosses
C2H2 zinc finger protein (C2H2-ZFP) plays an important role in regulating plant growth, development, and response to abiotic stress. To date, there have been no analyses of the C2H2-ZFP family in desiccation-tolerant moss. In this study, we identified 57 BaZFP transcripts across the <i>Bryum a...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-10-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/21/12894 |
| Summary: | C2H2 zinc finger protein (C2H2-ZFP) plays an important role in regulating plant growth, development, and response to abiotic stress. To date, there have been no analyses of the C2H2-ZFP family in desiccation-tolerant moss. In this study, we identified 57 BaZFP transcripts across the <i>Bryum argenteum</i> (<i>B. argenteum</i>) transcriptome. The BaZFP proteins were phylogenetically divided into four groups (I–IV). Additionally, we studied the <i>BaZFP1</i> gene, which is a nuclear C2H2-ZFP and acts as a positive regulator of growth and development in both moss and <i>Arabidopsis thaliana</i>. The complete coding sequence of the <i>BaZFP1</i> gene was isolated from <i>B. argenteum</i> cDNA, which showed a high expression level in a dehydration–rehydration treatment process. The overexpression of the <i>BaZFP1</i> gene in the <i>Physcomitrium patens</i> and <i>B. argenteum</i> promoted differentiation and growth of gametophytes. Heterologous expression in <i>Arabidopsis</i> regulated the whole growth and development cycle. In addition, we quantitatively analyzed the genes related to growth and development in transgenic moss and <i>Arabidopsis</i>, including <i>HLS1</i>, <i>HY5</i>, <i>ANT</i>, <i>LFY</i>, <i>FT</i>, <i>EIN3</i>, <i>MUS</i>, <i>APB4</i>, <i>SEC6</i>, and <i>STM1</i>, and found that their expression levels changed significantly. This study may pave the way for substantial insights into the role of C2H2-ZFPs in plants as well as suggest appropriate candidate genes for crop breeding. |
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| ISSN: | 1661-6596 1422-0067 |