Effect of Nrg1 Repressor on NTH1 Transcription and Molecular Docking of Nrg1 on NTH1 Promoter
The amount of intracellular trehalose increases in response to environmental stress in yeast (Saccharomyces cerevisiae). When that stress is terminated, the accumulated trehalose rapidly degrades into glucose rapidly. Synthesis of trehalose is fulfilled by the Trehalose Phosphate Synthase (TPS) en...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Universitas Indonesia
2019-09-01
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| Series: | Makara Journal of Science |
| Subjects: | |
| Online Access: | https://scholarhub.ui.ac.id/cgi/viewcontent.cgi?article=1008&context=science |
| Summary: | The amount of intracellular trehalose increases in response to environmental stress in yeast (Saccharomyces cerevisiae).
When that stress is terminated, the accumulated trehalose rapidly degrades into glucose rapidly. Synthesis of trehalose
is fulfilled by the Trehalose Phosphate Synthase (TPS) enzyme complex, whereas the degradation of trehalose is done
by the neutral trehalase enzyme. Under different stress conditions, transcription of the NTH1 gene is activated and
Stress Response Elements (STRE) are required for this activation. Nrg1 protein can bind promoters including STRE and
PDS elements. Because of the presence of three possible Nrg1 repressor binding sites on the NTH1 promoter, the NTH1
gene may be regulated by the Nrg1 repressor. In order to test this hypothesis, Δnrg1 mutant yeast and its isogenic wildtype yeast strain were used to analyze the transcriptional activation of the NTH1 gene under nitrogen starving
conditions. Nth1 transcription of the mutant yeast was seven-fold higher than that of the wild-type under growth
conditions, and was not changed during nitrogen starvation. The protein-DNA docking analysis also supported the
possibility of Nrg1 binding to the NTH1 promoter. These results revealed that NTH1 gene expression is constitutive in
the absence of the Nrg1 repressor protein, hence the transcription of NTH1 is repressed by the Nrg1 protein. |
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| ISSN: | 2339-1995 2356-0851 |