Revealing cell fate decisions during reprogramming by scRNA-seq
Single-cell RNA sequencing (scRNA-seq) technologies serve as powerful tools to dissect cellular heterogeneity comprehensively. With the rapid development of scRNA-seq, many previously unsolved questions were answered by using scRNA-seq. Cell reprogramming allows to reprogram the somatic cell into pl...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2020-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/05/e3sconf_iaecst2020_01033.pdf |
| _version_ | 1818445491239649280 |
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| author | Liang Yu |
| author_facet | Liang Yu |
| author_sort | Liang Yu |
| collection | DOAJ |
| description | Single-cell RNA sequencing (scRNA-seq) technologies serve as powerful tools to dissect cellular heterogeneity comprehensively. With the rapid development of scRNA-seq, many previously unsolved questions were answered by using scRNA-seq. Cell reprogramming allows to reprogram the somatic cell into pluripotent stem cells by specific transcription factors or small molecules. However, the underlying mechanism for the reprogramming progress remains unclear in some aspects for it is a highly heterogeneous process. By using scRNA-seq, it is of great value for better understanding the mechanism of reprogramming process by analyzing cell fate conversion at single-cell level. In this review, we will introduce the methods of scRNA-seq and generation of iPSCs by reprogramming, and summarize the main researches that revealing reprogramming mechanism with the use scRNA-seq. |
| first_indexed | 2024-12-14T19:32:40Z |
| format | Article |
| id | doaj.art-06f48f8b1046438cb81775e97d60e752 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-12-14T19:32:40Z |
| publishDate | 2020-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-06f48f8b1046438cb81775e97d60e7522022-12-21T22:50:01ZengEDP SciencesE3S Web of Conferences2267-12422020-01-011450103310.1051/e3sconf/202014501033e3sconf_iaecst2020_01033Revealing cell fate decisions during reprogramming by scRNA-seqLiang Yu0Human Aging Research Institute, School of Life Science, Nanchang UniversitySingle-cell RNA sequencing (scRNA-seq) technologies serve as powerful tools to dissect cellular heterogeneity comprehensively. With the rapid development of scRNA-seq, many previously unsolved questions were answered by using scRNA-seq. Cell reprogramming allows to reprogram the somatic cell into pluripotent stem cells by specific transcription factors or small molecules. However, the underlying mechanism for the reprogramming progress remains unclear in some aspects for it is a highly heterogeneous process. By using scRNA-seq, it is of great value for better understanding the mechanism of reprogramming process by analyzing cell fate conversion at single-cell level. In this review, we will introduce the methods of scRNA-seq and generation of iPSCs by reprogramming, and summarize the main researches that revealing reprogramming mechanism with the use scRNA-seq.https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/05/e3sconf_iaecst2020_01033.pdf |
| spellingShingle | Liang Yu Revealing cell fate decisions during reprogramming by scRNA-seq E3S Web of Conferences |
| title | Revealing cell fate decisions during reprogramming by scRNA-seq |
| title_full | Revealing cell fate decisions during reprogramming by scRNA-seq |
| title_fullStr | Revealing cell fate decisions during reprogramming by scRNA-seq |
| title_full_unstemmed | Revealing cell fate decisions during reprogramming by scRNA-seq |
| title_short | Revealing cell fate decisions during reprogramming by scRNA-seq |
| title_sort | revealing cell fate decisions during reprogramming by scrna seq |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2020/05/e3sconf_iaecst2020_01033.pdf |
| work_keys_str_mv | AT liangyu revealingcellfatedecisionsduringreprogrammingbyscrnaseq |