Optimization of in Vitro Carotenoid Production by Rhodotorula Toruloides
Carotenoids are widely researched due to their correlation with the mitigation of severe illnesses such as cancer, cardiovascular disease, and macular degeneration, which points out the critical significance of natural pigments. Carotenoid production from microorganisms has many advantages compared...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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AIDIC Servizi S.r.l.
2024-03-01
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| Series: | Chemical Engineering Transactions |
| Online Access: | https://www.cetjournal.it/index.php/cet/article/view/14420 |
| _version_ | 1827296398124515328 |
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| author | Thai M. Nguyen Trinh T.L. Nguyen Tu T. Nguyen Nhat N.N. Vo Nhan T. Vo Yen T.N. Nguyen |
| author_facet | Thai M. Nguyen Trinh T.L. Nguyen Tu T. Nguyen Nhat N.N. Vo Nhan T. Vo Yen T.N. Nguyen |
| author_sort | Thai M. Nguyen |
| collection | DOAJ |
| description | Carotenoids are widely researched due to their correlation with the mitigation of severe illnesses such as cancer, cardiovascular disease, and macular degeneration, which points out the critical significance of natural pigments. Carotenoid production from microorganisms has many advantages compared to plants or algae because of its rapid growth rate, cost-effectiveness, and independence from geographical factors. The genus Rhodosporidium, specifically the species Rhodosporidium toruloides, is renowned for generating carotenoid-rich biomass in biotechnology. However, the quantity and composition of carotenoid products obtained highly depend on the specific medium and culture conditions. In this research, carotenoid extraction by combining DMSO and acetone can result in high efficiency. Furthermore, the fermentation medium for R. toruloides was successfully optimized with affordable ingredients, including glucose, NaCl, H2O2, KH2PO4, MgSO4.7H2O, NH4Cl2, Na2HPO4, and yeast extract. The reassessment of the optimal model revealed strong compatibility between the predictive and experimental models regarding dry biomass and carotenoid content. The low residual glucose amount ultimately revealed adequate glucose consumption as a substrate for growth and carotenoid production. |
| first_indexed | 2024-04-24T14:38:05Z |
| format | Article |
| id | doaj.art-06f6a2b1161e4bfd83e0efaee05042ff |
| institution | Directory Open Access Journal |
| issn | 2283-9216 |
| language | English |
| last_indexed | 2024-04-24T14:38:05Z |
| publishDate | 2024-03-01 |
| publisher | AIDIC Servizi S.r.l. |
| record_format | Article |
| series | Chemical Engineering Transactions |
| spelling | doaj.art-06f6a2b1161e4bfd83e0efaee05042ff2024-04-02T23:01:16ZengAIDIC Servizi S.r.l.Chemical Engineering Transactions2283-92162024-03-0110810.3303/CET24108010Optimization of in Vitro Carotenoid Production by Rhodotorula ToruloidesThai M. NguyenTrinh T.L. NguyenTu T. NguyenNhat N.N. VoNhan T. VoYen T.N. NguyenCarotenoids are widely researched due to their correlation with the mitigation of severe illnesses such as cancer, cardiovascular disease, and macular degeneration, which points out the critical significance of natural pigments. Carotenoid production from microorganisms has many advantages compared to plants or algae because of its rapid growth rate, cost-effectiveness, and independence from geographical factors. The genus Rhodosporidium, specifically the species Rhodosporidium toruloides, is renowned for generating carotenoid-rich biomass in biotechnology. However, the quantity and composition of carotenoid products obtained highly depend on the specific medium and culture conditions. In this research, carotenoid extraction by combining DMSO and acetone can result in high efficiency. Furthermore, the fermentation medium for R. toruloides was successfully optimized with affordable ingredients, including glucose, NaCl, H2O2, KH2PO4, MgSO4.7H2O, NH4Cl2, Na2HPO4, and yeast extract. The reassessment of the optimal model revealed strong compatibility between the predictive and experimental models regarding dry biomass and carotenoid content. The low residual glucose amount ultimately revealed adequate glucose consumption as a substrate for growth and carotenoid production.https://www.cetjournal.it/index.php/cet/article/view/14420 |
| spellingShingle | Thai M. Nguyen Trinh T.L. Nguyen Tu T. Nguyen Nhat N.N. Vo Nhan T. Vo Yen T.N. Nguyen Optimization of in Vitro Carotenoid Production by Rhodotorula Toruloides Chemical Engineering Transactions |
| title | Optimization of in Vitro Carotenoid Production by Rhodotorula Toruloides |
| title_full | Optimization of in Vitro Carotenoid Production by Rhodotorula Toruloides |
| title_fullStr | Optimization of in Vitro Carotenoid Production by Rhodotorula Toruloides |
| title_full_unstemmed | Optimization of in Vitro Carotenoid Production by Rhodotorula Toruloides |
| title_short | Optimization of in Vitro Carotenoid Production by Rhodotorula Toruloides |
| title_sort | optimization of in vitro carotenoid production by rhodotorula toruloides |
| url | https://www.cetjournal.it/index.php/cet/article/view/14420 |
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