Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces
AbstractTermitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-03-01
|
| Series: | Mycobiology |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/12298093.2023.2187614 |
| _version_ | 1827957922229911552 |
|---|---|
| author | Suphachai Tharavecharak Corina N. D’Alessandro-Gabazza Masaaki Toda Taro Yasuma Taku Tsuyama Ichiro Kamei Esteban C. Gabazza |
| author_facet | Suphachai Tharavecharak Corina N. D’Alessandro-Gabazza Masaaki Toda Taro Yasuma Taku Tsuyama Ichiro Kamei Esteban C. Gabazza |
| author_sort | Suphachai Tharavecharak |
| collection | DOAJ |
| description | AbstractTermitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The study evaluated the optimal conditions for the artificial culture of Termitomyces and their inhibitory activity on AXL and immune checkpoint molecules in lung adenocarcinoma and melanoma cell lines. The culture of 45 strains of Termitomyces was compared. Five strains with marked growth rates were selected. Four of the selected strains form a single cluster by sequence analysis. The mycelium of 4 selected strains produces more fungal mass in potato dextrose broth than in a mixed media. The bark was the most appropriate solid substrate for Termitomyces mycelia culture. The mycelium of all five selected strains showed a higher growth rate under normal CO2 conditions. The culture broth, methanol, and ethyl acetate of one selected strain (T-120) inhibited the mRNA relative expression of AXL receptor tyrosine kinase and immune checkpoint molecules in cancer cell lines. Overall, these results suggest the potential usefulness of Termitomyces extracts as a co-adjuvant therapy in malignant diseases. |
| first_indexed | 2024-04-09T15:28:38Z |
| format | Article |
| id | doaj.art-06f6da928b214e1b90dad5e530ee86a1 |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-04-09T15:28:38Z |
| publishDate | 2023-03-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-06f6da928b214e1b90dad5e530ee86a12023-04-28T10:22:00ZengTaylor & Francis GroupMycobiology1229-80932092-93232023-03-015129410810.1080/12298093.2023.2187614Culture Conditions for Mycelial Growth and Anti-Cancer Properties of TermitomycesSuphachai Tharavecharak0Corina N. D’Alessandro-Gabazza1Masaaki Toda2Taro Yasuma3Taku Tsuyama4Ichiro Kamei5Esteban C. Gabazza6Graduate School of Agriculture, University of Miyazaki, Miyazaki, JapanDepartment of Immunology, Faculty of Medicine, Graduate School of Medicine, Mie University, Tsu, JapanDepartment of Immunology, Faculty of Medicine, Graduate School of Medicine, Mie University, Tsu, JapanDepartment of Immunology, Faculty of Medicine, Graduate School of Medicine, Mie University, Tsu, JapanGraduate School of Agriculture, University of Miyazaki, Miyazaki, JapanGraduate School of Agriculture, University of Miyazaki, Miyazaki, JapanDepartment of Immunology, Faculty of Medicine, Graduate School of Medicine, Mie University, Tsu, JapanAbstractTermitomyces sp. that grow in symbiosis with fungus-farming Termites have medicinal properties. However, they are rare in nature, and their artificial culture is challenging. The expression of AXL receptor tyrosine kinase and immune checkpoint molecules favor the growth of cancer cells. The study evaluated the optimal conditions for the artificial culture of Termitomyces and their inhibitory activity on AXL and immune checkpoint molecules in lung adenocarcinoma and melanoma cell lines. The culture of 45 strains of Termitomyces was compared. Five strains with marked growth rates were selected. Four of the selected strains form a single cluster by sequence analysis. The mycelium of 4 selected strains produces more fungal mass in potato dextrose broth than in a mixed media. The bark was the most appropriate solid substrate for Termitomyces mycelia culture. The mycelium of all five selected strains showed a higher growth rate under normal CO2 conditions. The culture broth, methanol, and ethyl acetate of one selected strain (T-120) inhibited the mRNA relative expression of AXL receptor tyrosine kinase and immune checkpoint molecules in cancer cell lines. Overall, these results suggest the potential usefulness of Termitomyces extracts as a co-adjuvant therapy in malignant diseases.https://www.tandfonline.com/doi/10.1080/12298093.2023.2187614Edible mushroomTermitomycesAXLPD-L1PD-L2artificial culture |
| spellingShingle | Suphachai Tharavecharak Corina N. D’Alessandro-Gabazza Masaaki Toda Taro Yasuma Taku Tsuyama Ichiro Kamei Esteban C. Gabazza Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces Mycobiology Edible mushroom Termitomyces AXL PD-L1 PD-L2 artificial culture |
| title | Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces |
| title_full | Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces |
| title_fullStr | Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces |
| title_full_unstemmed | Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces |
| title_short | Culture Conditions for Mycelial Growth and Anti-Cancer Properties of Termitomyces |
| title_sort | culture conditions for mycelial growth and anti cancer properties of termitomyces |
| topic | Edible mushroom Termitomyces AXL PD-L1 PD-L2 artificial culture |
| url | https://www.tandfonline.com/doi/10.1080/12298093.2023.2187614 |
| work_keys_str_mv | AT suphachaitharavecharak cultureconditionsformycelialgrowthandanticancerpropertiesoftermitomyces AT corinandalessandrogabazza cultureconditionsformycelialgrowthandanticancerpropertiesoftermitomyces AT masaakitoda cultureconditionsformycelialgrowthandanticancerpropertiesoftermitomyces AT taroyasuma cultureconditionsformycelialgrowthandanticancerpropertiesoftermitomyces AT takutsuyama cultureconditionsformycelialgrowthandanticancerpropertiesoftermitomyces AT ichirokamei cultureconditionsformycelialgrowthandanticancerpropertiesoftermitomyces AT estebancgabazza cultureconditionsformycelialgrowthandanticancerpropertiesoftermitomyces |