Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trial
ABSTRACT Introduction: Gut microbiota imbalance is linked to high uremic toxins production such as indole-3-acetic acid (IAA) in chronic kidney disease patients. This toxin can activate the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor involved with inflammation. Strategi...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Nefrologia
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| Series: | Brazilian Journal of Nephrology |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002020005015204&lng=en&tlng=en |
| _version_ | 1818974732363497472 |
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| author | Renata Azevedo Marta Esgalhado Julie Ann Kemp Bruna Regis Ludmila FMF Cardozo Lia S. Nakao Jessyca Sousa de Brito Denise Mafra |
| author_facet | Renata Azevedo Marta Esgalhado Julie Ann Kemp Bruna Regis Ludmila FMF Cardozo Lia S. Nakao Jessyca Sousa de Brito Denise Mafra |
| author_sort | Renata Azevedo |
| collection | DOAJ |
| description | ABSTRACT Introduction: Gut microbiota imbalance is linked to high uremic toxins production such as indole-3-acetic acid (IAA) in chronic kidney disease patients. This toxin can activate the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor involved with inflammation. Strategies to restore gut microbiota balance can be associated with reduced production of IAA and its deleterious effects. This study aimed to evaluate prebiotic resistant starch (RS) supplementation effects on IAA plasma levels and AhR mRNA expression in CKD patients on hemodialysis (HD). Methods: This randomized, double-blind and placebo-controlled clinical trial evaluated forty-two stable HD patients allocated in RS (n=22) or placebo (n=20) groups. Patients received, alternately, cookies and sachets containing 16 g/day of RS (Hi-Maize 260®) or manioc flour for four weeks. Fasting pre-dialysis blood samples were collected and IAA plasma levels measured by high performance liquid chromatography. Peripheral blood mononuclear cells were isolated and processed for AhR and nuclear factor kappa B (NF-κB) mRNA expression analyzes by quantitative real-time PCR. Anthropometric and biochemical parameters, as well as food intake were also evaluated. Results: Thirty-one patients completed the study, 15 in the RS group and 16 in the placebo group. Although there was no significant alteration in IAA plasma levels, neither in AhR mRNA expression and NF-κB mRNA expression after RS supplementation, a positive correlation (r=0.48; p=0.03) was observed between IAA plasma levels and AhR expression at baseline. Conclusion: Even though prebiotic RS supplementation did not influence IAA levels or AhR expression, their positive association reinforces a possible interaction between them. |
| first_indexed | 2024-12-20T15:44:44Z |
| format | Article |
| id | doaj.art-06faa0f1f3484182b84f2c89284d49ec |
| institution | Directory Open Access Journal |
| issn | 0101-2800 2175-8239 |
| language | English |
| last_indexed | 2024-12-20T15:44:44Z |
| publisher | Sociedade Brasileira de Nefrologia |
| record_format | Article |
| series | Brazilian Journal of Nephrology |
| spelling | doaj.art-06faa0f1f3484182b84f2c89284d49ec2022-12-21T19:35:02ZengSociedade Brasileira de NefrologiaBrazilian Journal of Nephrology0101-28002175-823910.1590/2175-8239-jbn-2020-0003S0101-28002020005015204Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trialRenata AzevedoMarta EsgalhadoJulie Ann KempBruna RegisLudmila FMF CardozoLia S. NakaoJessyca Sousa de BritoDenise MafraABSTRACT Introduction: Gut microbiota imbalance is linked to high uremic toxins production such as indole-3-acetic acid (IAA) in chronic kidney disease patients. This toxin can activate the aryl hydrocarbon receptor (AhR), a ligand-activated transcription factor involved with inflammation. Strategies to restore gut microbiota balance can be associated with reduced production of IAA and its deleterious effects. This study aimed to evaluate prebiotic resistant starch (RS) supplementation effects on IAA plasma levels and AhR mRNA expression in CKD patients on hemodialysis (HD). Methods: This randomized, double-blind and placebo-controlled clinical trial evaluated forty-two stable HD patients allocated in RS (n=22) or placebo (n=20) groups. Patients received, alternately, cookies and sachets containing 16 g/day of RS (Hi-Maize 260®) or manioc flour for four weeks. Fasting pre-dialysis blood samples were collected and IAA plasma levels measured by high performance liquid chromatography. Peripheral blood mononuclear cells were isolated and processed for AhR and nuclear factor kappa B (NF-κB) mRNA expression analyzes by quantitative real-time PCR. Anthropometric and biochemical parameters, as well as food intake were also evaluated. Results: Thirty-one patients completed the study, 15 in the RS group and 16 in the placebo group. Although there was no significant alteration in IAA plasma levels, neither in AhR mRNA expression and NF-κB mRNA expression after RS supplementation, a positive correlation (r=0.48; p=0.03) was observed between IAA plasma levels and AhR expression at baseline. Conclusion: Even though prebiotic RS supplementation did not influence IAA levels or AhR expression, their positive association reinforces a possible interaction between them.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002020005015204&lng=en&tlng=enprebioticsaryl hydrocarbon receptorinflammationchronic kidney diseasehemodialysis |
| spellingShingle | Renata Azevedo Marta Esgalhado Julie Ann Kemp Bruna Regis Ludmila FMF Cardozo Lia S. Nakao Jessyca Sousa de Brito Denise Mafra Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trial Brazilian Journal of Nephrology prebiotics aryl hydrocarbon receptor inflammation chronic kidney disease hemodialysis |
| title | Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trial |
| title_full | Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trial |
| title_fullStr | Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trial |
| title_full_unstemmed | Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trial |
| title_short | Resistant starch supplementation effects on plasma indole 3-acetic acid and aryl hydrocarbon receptor mRNA expression in hemodialysis patients: Randomized, double blind and controlled clinical trial |
| title_sort | resistant starch supplementation effects on plasma indole 3 acetic acid and aryl hydrocarbon receptor mrna expression in hemodialysis patients randomized double blind and controlled clinical trial |
| topic | prebiotics aryl hydrocarbon receptor inflammation chronic kidney disease hemodialysis |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-28002020005015204&lng=en&tlng=en |
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