Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60
Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy vo...
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| Format: | Article |
| Language: | English |
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Pontificia Universidad Javeriana
2009-04-01
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| Series: | Universitas Scientiarum |
| Subjects: | |
| Online Access: | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860 |
| _version_ | 1819277813014855680 |
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| author | Martha Mesa-Villanueva Magaly Rueda-Martínez Ruby Zayas-Camacho Diana Sicard-Suárez |
| author_facet | Martha Mesa-Villanueva Magaly Rueda-Martínez Ruby Zayas-Camacho Diana Sicard-Suárez |
| author_sort | Martha Mesa-Villanueva |
| collection | DOAJ |
| description | Protective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy volunteers were incubated with 0, 1x10-5, 1x10-6 y 1x10-7 M melatonin for 10 minutes beforebeing exposed to gamma radiation (300 cGy; Co60 source). Afterwards, DNA damage was evaluated with the comet assay. Results:PBMCs pretreated with melatonin showed comet tails shorter than those without the hormone treatment, as well as a lower percentage ofcells with severe DNA damage. Conclusion: melatonin doses of 1x10-5, 1x10-6 and 1x10-7 M provide in vitro protection to PBMCs fromDNA damage (single strand breaks and alkali-labile sites) induced by gamma radiation (300 cGy; Co60 source). |
| first_indexed | 2024-12-24T00:02:04Z |
| format | Article |
| id | doaj.art-06faed05b79b4e878ce4f2aa09656c38 |
| institution | Directory Open Access Journal |
| issn | 0122-7483 2027-1352 |
| language | English |
| last_indexed | 2024-12-24T00:02:04Z |
| publishDate | 2009-04-01 |
| publisher | Pontificia Universidad Javeriana |
| record_format | Article |
| series | Universitas Scientiarum |
| spelling | doaj.art-06faed05b79b4e878ce4f2aa09656c382022-12-21T17:25:05ZengPontificia Universidad JaverianaUniversitas Scientiarum0122-74832027-13522009-04-011414147Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60Martha Mesa-VillanuevaMagaly Rueda-MartínezRuby Zayas-CamachoDiana Sicard-SuárezProtective action of melatonin on human peripheral blood mononuclear cells exposed to gamma radiation Co60. Objective: toevaluate the protective effect of melatonin on peripheral blood mononuclear cells (PBMCs) exposed in vitro to ionizing radiation. Materialsand methods: PBMCs drawn from healthy volunteers were incubated with 0, 1x10-5, 1x10-6 y 1x10-7 M melatonin for 10 minutes beforebeing exposed to gamma radiation (300 cGy; Co60 source). Afterwards, DNA damage was evaluated with the comet assay. Results:PBMCs pretreated with melatonin showed comet tails shorter than those without the hormone treatment, as well as a lower percentage ofcells with severe DNA damage. Conclusion: melatonin doses of 1x10-5, 1x10-6 and 1x10-7 M provide in vitro protection to PBMCs fromDNA damage (single strand breaks and alkali-labile sites) induced by gamma radiation (300 cGy; Co60 source).http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860blood mononuclear cellscomet assayDNA damagegamma radiationmelatonin |
| spellingShingle | Martha Mesa-Villanueva Magaly Rueda-Martínez Ruby Zayas-Camacho Diana Sicard-Suárez Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 Universitas Scientiarum blood mononuclear cells comet assay DNA damage gamma radiation melatonin |
| title | Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
| title_full | Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
| title_fullStr | Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
| title_full_unstemmed | Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
| title_short | Acción protectora de la melatonina sobre células mononucleares de sangre periférica humana sometidas a radiación gamma Co60 |
| title_sort | accion protectora de la melatonina sobre celulas mononucleares de sangre periferica humana sometidas a radiacion gamma co60 |
| topic | blood mononuclear cells comet assay DNA damage gamma radiation melatonin |
| url | http://revistas.javeriana.edu.co/index.php/scientarium/article/view/1398/860 |
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