Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)
Aspergillus flavus is an opportunistic fungal pathogen that produces carcinogenic aflatoxin, a serious constraint for food safety and human health. In this study, to better understand the molecular mechanism/s of peanut resistance to A. flavus growth and aflatoxin accumulation, comparative proteomic...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Journal of Plant Interactions |
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| Online Access: | http://dx.doi.org/10.1080/17429145.2021.1995058 |
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| author | Pooja Bhatnagar-Mathur Kalenahalli Yogendra Santisree Parankusam Hemlatha Sanivarapu Kalyani Prasad Shiva Bhargavi Lingampali Kiran Kumar Sharma |
| author_facet | Pooja Bhatnagar-Mathur Kalenahalli Yogendra Santisree Parankusam Hemlatha Sanivarapu Kalyani Prasad Shiva Bhargavi Lingampali Kiran Kumar Sharma |
| author_sort | Pooja Bhatnagar-Mathur |
| collection | DOAJ |
| description | Aspergillus flavus is an opportunistic fungal pathogen that produces carcinogenic aflatoxin, a serious constraint for food safety and human health. In this study, to better understand the molecular mechanism/s of peanut resistance to A. flavus growth and aflatoxin accumulation, comparative proteomic analysis was performed in two contrasting peanut genotypes, variety JL 24 (susceptible) and its near-isogenic resistant transgenic derivative expressing an alfalfa defensin gene. Several resistance proteins associated with secondary metabolic pathways were strongly induced in the resistant genotypes including phenylalanine ammonia lyase, cinnamic acid-4-hydroxylase, chalcone synthase, resveratrol synthase, flavanone-3-hydroxylase, lipoxygenase, diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase, β-ketoacyl-ACP-reductase, monoacylglycerol acyltransferase, and diacylglycerol acyltransferase, indicating their roles in resistance. Besides, several putative susceptibility-associated proteins were revealed providing knowledge on potential candidate target genes for precise breeding interventions for aflatoxin mitigation. This is the first study to demonstrate comparative proteomics analysis in Aspergillus–peanut interaction using contrasting near-isogenic lines to elucidate the underlying molecular mechanisms of resistance. |
| first_indexed | 2024-12-24T01:25:09Z |
| format | Article |
| id | doaj.art-06fbb99cbffc4291a41750c02519becf |
| institution | Directory Open Access Journal |
| issn | 1742-9145 1742-9153 |
| language | English |
| last_indexed | 2024-12-24T01:25:09Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj.art-06fbb99cbffc4291a41750c02519becf2022-12-21T17:22:32ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532021-01-0116149450910.1080/17429145.2021.19950581995058Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.)Pooja Bhatnagar-Mathur0Kalenahalli Yogendra1Santisree Parankusam2Hemlatha Sanivarapu3Kalyani Prasad4Shiva Bhargavi Lingampali5Kiran Kumar Sharma6International Crop Research Institute for the Semi-Arid Tropics (ICRISAT)International Crop Research Institute for the Semi-Arid Tropics (ICRISAT)International Crop Research Institute for the Semi-Arid Tropics (ICRISAT)International Crop Research Institute for the Semi-Arid Tropics (ICRISAT)International Crop Research Institute for the Semi-Arid Tropics (ICRISAT)International Crop Research Institute for the Semi-Arid Tropics (ICRISAT)International Crop Research Institute for the Semi-Arid Tropics (ICRISAT)Aspergillus flavus is an opportunistic fungal pathogen that produces carcinogenic aflatoxin, a serious constraint for food safety and human health. In this study, to better understand the molecular mechanism/s of peanut resistance to A. flavus growth and aflatoxin accumulation, comparative proteomic analysis was performed in two contrasting peanut genotypes, variety JL 24 (susceptible) and its near-isogenic resistant transgenic derivative expressing an alfalfa defensin gene. Several resistance proteins associated with secondary metabolic pathways were strongly induced in the resistant genotypes including phenylalanine ammonia lyase, cinnamic acid-4-hydroxylase, chalcone synthase, resveratrol synthase, flavanone-3-hydroxylase, lipoxygenase, diacylglycerol-glycerol-3-phosphate-3-phosphatidyltransferase, β-ketoacyl-ACP-reductase, monoacylglycerol acyltransferase, and diacylglycerol acyltransferase, indicating their roles in resistance. Besides, several putative susceptibility-associated proteins were revealed providing knowledge on potential candidate target genes for precise breeding interventions for aflatoxin mitigation. This is the first study to demonstrate comparative proteomics analysis in Aspergillus–peanut interaction using contrasting near-isogenic lines to elucidate the underlying molecular mechanisms of resistance.http://dx.doi.org/10.1080/17429145.2021.1995058aflatoxinarachis hypogaeaaspergillus flavusflavonoidspeanutproteomicsphenylpropanoids |
| spellingShingle | Pooja Bhatnagar-Mathur Kalenahalli Yogendra Santisree Parankusam Hemlatha Sanivarapu Kalyani Prasad Shiva Bhargavi Lingampali Kiran Kumar Sharma Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.) Journal of Plant Interactions aflatoxin arachis hypogaea aspergillus flavus flavonoids peanut proteomics phenylpropanoids |
| title | Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.) |
| title_full | Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.) |
| title_fullStr | Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.) |
| title_full_unstemmed | Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.) |
| title_short | Comparative proteomics provide insights on the basis of resistance to Aspergillus flavus infection and aflatoxin production in peanut (Arachis hypogea L.) |
| title_sort | comparative proteomics provide insights on the basis of resistance to aspergillus flavus infection and aflatoxin production in peanut arachis hypogea l |
| topic | aflatoxin arachis hypogaea aspergillus flavus flavonoids peanut proteomics phenylpropanoids |
| url | http://dx.doi.org/10.1080/17429145.2021.1995058 |
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