Confirmation value of Western blotting in detecting anti-treponema pallidum specific antibodies with suspicious results
Abstract Background Due to the inconsistent results of anti-treponema pallidum (TP) specific antibodies by enzyme-linked immunosorbent assay (ELISA) and Treponema pallidum granule agglutination assay (TPPA) in clinical work, there will be a certain proportion of false-positives and false-negatives d...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-02-01
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| Series: | European Journal of Medical Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s40001-022-00633-y |
| _version_ | 1828424741859360768 |
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| author | Siqi Xu Hongsheng Li Xiaoyan Wu Jianwei Guo Jiaoli Zhang Xuqi Hu |
| author_facet | Siqi Xu Hongsheng Li Xiaoyan Wu Jianwei Guo Jiaoli Zhang Xuqi Hu |
| author_sort | Siqi Xu |
| collection | DOAJ |
| description | Abstract Background Due to the inconsistent results of anti-treponema pallidum (TP) specific antibodies by enzyme-linked immunosorbent assay (ELISA) and Treponema pallidum granule agglutination assay (TPPA) in clinical work, there will be a certain proportion of false-positives and false-negatives depending on TPPA as confirmation results. This study aimed to evaluate the necessity of Western blotting (WB) in samples with inconsistent results in detecting anti-TP antibodies by ELISA and TPPA. Methods Specific anti-TP test results in our clinical laboratory were retrospectively analyzed. The specimens with a positive or a negative result, but with colored ELISA plates, were retested by TPPA. WB was used to confirm the suspicious results between ELISA and TPPA. The Chi-square test was used to analyze whether the difference was statistically significant. Results A total of 106,757 anti-TP specimens were screened by ELISA from August 2018 to December 2019; 3972 were retested by TPPA, and 3809 were positive by TPPA. ELISA and TPPA showed different results in 163 specimens. Among them, 29 specimens were negative and 134 were positive by ELISA; 76 were negative, 23 were positive, and 64 were “reserve” by TPPA; 93 were negative, 31 were positive, and 39 were suspicious by the WB confirmation test. Compared with WB, the difference in the results of ELISA and TPPA was statistically significant. Conclusions TPPA is an effective retest method for anti-TP antibody detection. If the results of anti-TP antibodies by ELISA and TPPA are inconsistent, it is necessary to use WB for confirmation. Trial registration This retrospective analysis is in accordance with the ethical guidelines of China and approved by the second hospital of Jiaxing (jxey-2018048). |
| first_indexed | 2024-12-10T16:22:18Z |
| format | Article |
| id | doaj.art-06fd545269334d2f9a897dff5675072b |
| institution | Directory Open Access Journal |
| issn | 2047-783X |
| language | English |
| last_indexed | 2024-12-10T16:22:18Z |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | European Journal of Medical Research |
| spelling | doaj.art-06fd545269334d2f9a897dff5675072b2022-12-22T01:41:46ZengBMCEuropean Journal of Medical Research2047-783X2022-02-012711610.1186/s40001-022-00633-yConfirmation value of Western blotting in detecting anti-treponema pallidum specific antibodies with suspicious resultsSiqi Xu0Hongsheng Li1Xiaoyan Wu2Jianwei Guo3Jiaoli Zhang4Xuqi Hu5The Second Hospital of JiaxingThe Second Hospital of JiaxingThe Second Hospital of JiaxingThe Second Hospital of JiaxingThe Second Hospital of JiaxingThe Second Hospital of JiaxingAbstract Background Due to the inconsistent results of anti-treponema pallidum (TP) specific antibodies by enzyme-linked immunosorbent assay (ELISA) and Treponema pallidum granule agglutination assay (TPPA) in clinical work, there will be a certain proportion of false-positives and false-negatives depending on TPPA as confirmation results. This study aimed to evaluate the necessity of Western blotting (WB) in samples with inconsistent results in detecting anti-TP antibodies by ELISA and TPPA. Methods Specific anti-TP test results in our clinical laboratory were retrospectively analyzed. The specimens with a positive or a negative result, but with colored ELISA plates, were retested by TPPA. WB was used to confirm the suspicious results between ELISA and TPPA. The Chi-square test was used to analyze whether the difference was statistically significant. Results A total of 106,757 anti-TP specimens were screened by ELISA from August 2018 to December 2019; 3972 were retested by TPPA, and 3809 were positive by TPPA. ELISA and TPPA showed different results in 163 specimens. Among them, 29 specimens were negative and 134 were positive by ELISA; 76 were negative, 23 were positive, and 64 were “reserve” by TPPA; 93 were negative, 31 were positive, and 39 were suspicious by the WB confirmation test. Compared with WB, the difference in the results of ELISA and TPPA was statistically significant. Conclusions TPPA is an effective retest method for anti-TP antibody detection. If the results of anti-TP antibodies by ELISA and TPPA are inconsistent, it is necessary to use WB for confirmation. Trial registration This retrospective analysis is in accordance with the ethical guidelines of China and approved by the second hospital of Jiaxing (jxey-2018048).https://doi.org/10.1186/s40001-022-00633-yELISATPPATreponema pallidum antibodyWestern blotting |
| spellingShingle | Siqi Xu Hongsheng Li Xiaoyan Wu Jianwei Guo Jiaoli Zhang Xuqi Hu Confirmation value of Western blotting in detecting anti-treponema pallidum specific antibodies with suspicious results European Journal of Medical Research ELISA TPPA Treponema pallidum antibody Western blotting |
| title | Confirmation value of Western blotting in detecting anti-treponema pallidum specific antibodies with suspicious results |
| title_full | Confirmation value of Western blotting in detecting anti-treponema pallidum specific antibodies with suspicious results |
| title_fullStr | Confirmation value of Western blotting in detecting anti-treponema pallidum specific antibodies with suspicious results |
| title_full_unstemmed | Confirmation value of Western blotting in detecting anti-treponema pallidum specific antibodies with suspicious results |
| title_short | Confirmation value of Western blotting in detecting anti-treponema pallidum specific antibodies with suspicious results |
| title_sort | confirmation value of western blotting in detecting anti treponema pallidum specific antibodies with suspicious results |
| topic | ELISA TPPA Treponema pallidum antibody Western blotting |
| url | https://doi.org/10.1186/s40001-022-00633-y |
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