Co-Doped CeO<sub>2</sub>/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of H<sub>2</sub>O<sub>2</sub> via Peroxidase Mimicking
Hydrogen peroxide acts as a byproduct of oxidative metabolism, and oxidative stress caused by its excess amount, causes different types of cancer. Thus, fast and cost-friendly analytical methods need to be developed for H<sub>2</sub>O<sub>2</sub>. Ionic liquid (IL)-coated cob...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/8/3325 |
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| author | Abdul Khaliq Ruqia Nazir Muslim Khan Abdur Rahim Muhammad Asad Mohibullah Shah Mansoor Khan Riaz Ullah Essam A. Ali Ajmir Khan Umar Nishan |
| author_facet | Abdul Khaliq Ruqia Nazir Muslim Khan Abdur Rahim Muhammad Asad Mohibullah Shah Mansoor Khan Riaz Ullah Essam A. Ali Ajmir Khan Umar Nishan |
| author_sort | Abdul Khaliq |
| collection | DOAJ |
| description | Hydrogen peroxide acts as a byproduct of oxidative metabolism, and oxidative stress caused by its excess amount, causes different types of cancer. Thus, fast and cost-friendly analytical methods need to be developed for H<sub>2</sub>O<sub>2</sub>. Ionic liquid (IL)-coated cobalt (Co)-doped cerium oxide (CeO<sub>2</sub>)/activated carbon (C) nanocomposite has been used to assess the peroxidase-like activity for the colorimetric detection of H<sub>2</sub>O<sub>2</sub>. Both activated C and IL have a synergistic effect on the electrical conductivity of the nanocomposites to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The Co-doped CeO<sub>2</sub>/activated C nanocomposite has been synthesized by the co-precipitation method and characterized by UV–Vis spectrophotometry, FTIR, SEM, EDX, Raman spectroscopy, and XRD. The prepared nanocomposite was functionalized with IL to avoid agglomeration. H<sub>2</sub>O<sub>2</sub> concentration, incubation time, pH, TMB concentration, and quantity of the capped nanocomposite were tuned. The proposed sensing probe gave a limit of detection of 1.3 × 10<sup>−8</sup> M, a limit of quantification of 1.4 × 10<sup>−8</sup> M, and an R<sup>2</sup> of 0.999. The sensor gave a colorimetric response within 2 min at pH 6 at room temperature. The co-existing species did not show any interference during the sensing probe. The proposed sensor showed high sensitivity and selectivity and was used to detect H<sub>2</sub>O<sub>2</sub> in cancer patients’ urine samples. |
| first_indexed | 2024-03-11T04:42:08Z |
| format | Article |
| id | doaj.art-06ff76d6e764480c828e154244eff810 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:42:08Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-06ff76d6e764480c828e154244eff8102023-11-17T20:37:22ZengMDPI AGMolecules1420-30492023-04-01288332510.3390/molecules28083325Co-Doped CeO<sub>2</sub>/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of H<sub>2</sub>O<sub>2</sub> via Peroxidase MimickingAbdul Khaliq0Ruqia Nazir1Muslim Khan2Abdur Rahim3Muhammad Asad4Mohibullah Shah5Mansoor Khan6Riaz Ullah7Essam A. Ali8Ajmir Khan9Umar Nishan10Department of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Chemistry, COMSATS University Islamabad, Park Road, Islamabad 45550, PakistanDepartment of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Biochemistry, Bahauddin Zakariya University, Multan 66000, PakistanDepartment of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanDepartment of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaDepartment of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh 11451, Saudi ArabiaSchool of Packaging, 448 Wilson Road, Michigan State University, East Lansing, MI 48824, USADepartment of Chemistry, Kohat University of Science and Technology, Kohat 26000, PakistanHydrogen peroxide acts as a byproduct of oxidative metabolism, and oxidative stress caused by its excess amount, causes different types of cancer. Thus, fast and cost-friendly analytical methods need to be developed for H<sub>2</sub>O<sub>2</sub>. Ionic liquid (IL)-coated cobalt (Co)-doped cerium oxide (CeO<sub>2</sub>)/activated carbon (C) nanocomposite has been used to assess the peroxidase-like activity for the colorimetric detection of H<sub>2</sub>O<sub>2</sub>. Both activated C and IL have a synergistic effect on the electrical conductivity of the nanocomposites to catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB). The Co-doped CeO<sub>2</sub>/activated C nanocomposite has been synthesized by the co-precipitation method and characterized by UV–Vis spectrophotometry, FTIR, SEM, EDX, Raman spectroscopy, and XRD. The prepared nanocomposite was functionalized with IL to avoid agglomeration. H<sub>2</sub>O<sub>2</sub> concentration, incubation time, pH, TMB concentration, and quantity of the capped nanocomposite were tuned. The proposed sensing probe gave a limit of detection of 1.3 × 10<sup>−8</sup> M, a limit of quantification of 1.4 × 10<sup>−8</sup> M, and an R<sup>2</sup> of 0.999. The sensor gave a colorimetric response within 2 min at pH 6 at room temperature. The co-existing species did not show any interference during the sensing probe. The proposed sensor showed high sensitivity and selectivity and was used to detect H<sub>2</sub>O<sub>2</sub> in cancer patients’ urine samples.https://www.mdpi.com/1420-3049/28/8/3325H<sub>2</sub>O<sub>2</sub>Co-doped CeO<sub>2</sub>/activated C nanocompositeionic liquidperoxidase mimiccolorimetric sensor |
| spellingShingle | Abdul Khaliq Ruqia Nazir Muslim Khan Abdur Rahim Muhammad Asad Mohibullah Shah Mansoor Khan Riaz Ullah Essam A. Ali Ajmir Khan Umar Nishan Co-Doped CeO<sub>2</sub>/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of H<sub>2</sub>O<sub>2</sub> via Peroxidase Mimicking Molecules H<sub>2</sub>O<sub>2</sub> Co-doped CeO<sub>2</sub>/activated C nanocomposite ionic liquid peroxidase mimic colorimetric sensor |
| title | Co-Doped CeO<sub>2</sub>/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of H<sub>2</sub>O<sub>2</sub> via Peroxidase Mimicking |
| title_full | Co-Doped CeO<sub>2</sub>/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of H<sub>2</sub>O<sub>2</sub> via Peroxidase Mimicking |
| title_fullStr | Co-Doped CeO<sub>2</sub>/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of H<sub>2</sub>O<sub>2</sub> via Peroxidase Mimicking |
| title_full_unstemmed | Co-Doped CeO<sub>2</sub>/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of H<sub>2</sub>O<sub>2</sub> via Peroxidase Mimicking |
| title_short | Co-Doped CeO<sub>2</sub>/Activated C Nanocomposite Functionalized with Ionic Liquid for Colorimetric Biosensing of H<sub>2</sub>O<sub>2</sub> via Peroxidase Mimicking |
| title_sort | co doped ceo sub 2 sub activated c nanocomposite functionalized with ionic liquid for colorimetric biosensing of h sub 2 sub o sub 2 sub via peroxidase mimicking |
| topic | H<sub>2</sub>O<sub>2</sub> Co-doped CeO<sub>2</sub>/activated C nanocomposite ionic liquid peroxidase mimic colorimetric sensor |
| url | https://www.mdpi.com/1420-3049/28/8/3325 |
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