High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis.
The recently discovered Type VII/Esat-6 secretion systems seem to be widespread among bacteria of the phyla Actinobacteria and Firmicutes. In some species they play an important role in pathogenic interactions with eukaryotic hosts. Several studies have predicted that the locus yukEDCByueBC of the n...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2013-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3701619?pdf=render |
| _version_ | 1811218743685021696 |
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| author | Catarina Baptista Hugo Condessa Barreto Carlos São-José |
| author_facet | Catarina Baptista Hugo Condessa Barreto Carlos São-José |
| author_sort | Catarina Baptista |
| collection | DOAJ |
| description | The recently discovered Type VII/Esat-6 secretion systems seem to be widespread among bacteria of the phyla Actinobacteria and Firmicutes. In some species they play an important role in pathogenic interactions with eukaryotic hosts. Several studies have predicted that the locus yukEDCByueBC of the non-pathogenic, Gram-positive bacterium Bacillus subtilis would encode an Esat-6-like secretion system (Ess). We provide here for the first time evidences for the functioning of this secretion pathway in an undomesticated B. subtilis strain. We show that YukE, a small protein with the typical features of the secretion substrates from the WXG100 superfamily is actively secreted to culture media. YukE secretion depends on intact yukDCByueBC genes, whose products share sequence or structural homology with known components of the S. aureus Ess. Biochemical characterization of YukE indicates that it exists as a dimer both in vitro and in vivo. We also show that the B. subtilis Ess essentially operates in late stationary growth phase in absolute dependence of phosphorylated DegU, the response regulator of the two-component system DegS-DegU. We present possible reasons that eventually have precluded the study of this secretion system in the B. subtilis laboratory strain 168. |
| first_indexed | 2024-04-12T07:14:33Z |
| format | Article |
| id | doaj.art-06ffaf03247c4e88ade3fc83f7efaee2 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T07:14:33Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-06ffaf03247c4e88ade3fc83f7efaee22022-12-22T03:42:30ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0187e6784010.1371/journal.pone.0067840High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis.Catarina BaptistaHugo Condessa BarretoCarlos São-JoséThe recently discovered Type VII/Esat-6 secretion systems seem to be widespread among bacteria of the phyla Actinobacteria and Firmicutes. In some species they play an important role in pathogenic interactions with eukaryotic hosts. Several studies have predicted that the locus yukEDCByueBC of the non-pathogenic, Gram-positive bacterium Bacillus subtilis would encode an Esat-6-like secretion system (Ess). We provide here for the first time evidences for the functioning of this secretion pathway in an undomesticated B. subtilis strain. We show that YukE, a small protein with the typical features of the secretion substrates from the WXG100 superfamily is actively secreted to culture media. YukE secretion depends on intact yukDCByueBC genes, whose products share sequence or structural homology with known components of the S. aureus Ess. Biochemical characterization of YukE indicates that it exists as a dimer both in vitro and in vivo. We also show that the B. subtilis Ess essentially operates in late stationary growth phase in absolute dependence of phosphorylated DegU, the response regulator of the two-component system DegS-DegU. We present possible reasons that eventually have precluded the study of this secretion system in the B. subtilis laboratory strain 168.http://europepmc.org/articles/PMC3701619?pdf=render |
| spellingShingle | Catarina Baptista Hugo Condessa Barreto Carlos São-José High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis. PLoS ONE |
| title | High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis. |
| title_full | High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis. |
| title_fullStr | High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis. |
| title_full_unstemmed | High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis. |
| title_short | High levels of DegU-P activate an Esat-6-like secretion system in Bacillus subtilis. |
| title_sort | high levels of degu p activate an esat 6 like secretion system in bacillus subtilis |
| url | http://europepmc.org/articles/PMC3701619?pdf=render |
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