The Bifunctional Catalase-Phenol Oxidase of Mycothermus Thermophilum (MtCATPO) Increases the Antioxidant Capacities of its Ortho-Diphenolic Substrates and of Green and Black Tea Extracts
Catalase from the thermophilic fungus Mycothermus thermophilus is a bifunctional enzyme with a secondary phenol oxidase activity (CATPO). MtCATPO catalyses the oxidation of catechol, chlorogenic acid, caffeic acid and (+)-catechin to yield mainly dimers, and higher molecular weight oligomers and pol...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2021-04-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
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| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3997 |
| _version_ | 1797906355802079232 |
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| author | Betül Söyler Zümrüt Begüm Ögel |
| author_facet | Betül Söyler Zümrüt Begüm Ögel |
| author_sort | Betül Söyler |
| collection | DOAJ |
| description | Catalase from the thermophilic fungus Mycothermus thermophilus is a bifunctional enzyme with a secondary phenol oxidase activity (CATPO). MtCATPO catalyses the oxidation of catechol, chlorogenic acid, caffeic acid and (+)-catechin to yield mainly dimers, and higher molecular weight oligomers and polymers. The role of this phenol oxidase activity is not known. Here, the antioxidant capacities (AC) of the phenolic substrates in the absence and presence of MtCATPO were compared. The oxidized products displayed enhanced AC reaching a maximum of 2.4-fold with catechol. Other phenol oxidases (laccase and tyrosinase) did not yield AC enhancement to the same extent. MtCATPO-treatment increased AC of green and black tea infusions, as well as water extracts of samples collected from a black tea production line up to 28%. The bifunctional MtCATPO appears to be an important antioxidant enzyme with a wide range of potential applications in the food, neutraceuticals and pharmaceuticals industry. |
| first_indexed | 2024-04-10T10:20:43Z |
| format | Article |
| id | doaj.art-06ffc05059b541388e6a8d3371a9524a |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T10:20:43Z |
| publishDate | 2021-04-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-06ffc05059b541388e6a8d3371a9524a2023-02-15T16:21:39ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2021-04-019468969510.24925/turjaf.v9i4.689-695.39972043The Bifunctional Catalase-Phenol Oxidase of Mycothermus Thermophilum (MtCATPO) Increases the Antioxidant Capacities of its Ortho-Diphenolic Substrates and of Green and Black Tea ExtractsBetül Söyler0Zümrüt Begüm Ögel1Department of Food Engineering, Middle East Technical University, 06800 AnkaraDepartment of Food Engineering, Konya Food and Agriculture University, 42080 KonyaCatalase from the thermophilic fungus Mycothermus thermophilus is a bifunctional enzyme with a secondary phenol oxidase activity (CATPO). MtCATPO catalyses the oxidation of catechol, chlorogenic acid, caffeic acid and (+)-catechin to yield mainly dimers, and higher molecular weight oligomers and polymers. The role of this phenol oxidase activity is not known. Here, the antioxidant capacities (AC) of the phenolic substrates in the absence and presence of MtCATPO were compared. The oxidized products displayed enhanced AC reaching a maximum of 2.4-fold with catechol. Other phenol oxidases (laccase and tyrosinase) did not yield AC enhancement to the same extent. MtCATPO-treatment increased AC of green and black tea infusions, as well as water extracts of samples collected from a black tea production line up to 28%. The bifunctional MtCATPO appears to be an important antioxidant enzyme with a wide range of potential applications in the food, neutraceuticals and pharmaceuticals industry.http://www.agrifoodscience.com/index.php/TURJAF/article/view/3997catalasephenol oxidasephenolic compoundsoxidationfood phenolicstea |
| spellingShingle | Betül Söyler Zümrüt Begüm Ögel The Bifunctional Catalase-Phenol Oxidase of Mycothermus Thermophilum (MtCATPO) Increases the Antioxidant Capacities of its Ortho-Diphenolic Substrates and of Green and Black Tea Extracts Turkish Journal of Agriculture: Food Science and Technology catalase phenol oxidase phenolic compounds oxidation food phenolics tea |
| title | The Bifunctional Catalase-Phenol Oxidase of Mycothermus Thermophilum (MtCATPO) Increases the Antioxidant Capacities of its Ortho-Diphenolic Substrates and of Green and Black Tea Extracts |
| title_full | The Bifunctional Catalase-Phenol Oxidase of Mycothermus Thermophilum (MtCATPO) Increases the Antioxidant Capacities of its Ortho-Diphenolic Substrates and of Green and Black Tea Extracts |
| title_fullStr | The Bifunctional Catalase-Phenol Oxidase of Mycothermus Thermophilum (MtCATPO) Increases the Antioxidant Capacities of its Ortho-Diphenolic Substrates and of Green and Black Tea Extracts |
| title_full_unstemmed | The Bifunctional Catalase-Phenol Oxidase of Mycothermus Thermophilum (MtCATPO) Increases the Antioxidant Capacities of its Ortho-Diphenolic Substrates and of Green and Black Tea Extracts |
| title_short | The Bifunctional Catalase-Phenol Oxidase of Mycothermus Thermophilum (MtCATPO) Increases the Antioxidant Capacities of its Ortho-Diphenolic Substrates and of Green and Black Tea Extracts |
| title_sort | bifunctional catalase phenol oxidase of mycothermus thermophilum mtcatpo increases the antioxidant capacities of its ortho diphenolic substrates and of green and black tea extracts |
| topic | catalase phenol oxidase phenolic compounds oxidation food phenolics tea |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/3997 |
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