Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian Patient
Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults, with few effective treatment strategies. The research on the development of new treatments is often constrained by the limitations of preclinical models, which fail to accurately replicate the disease’s essential cha...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/21/15861 |
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| author | Fernanda F. da Silva Fernanda C. S. Lupinacci Bruno D. S. Elias Adriano O. Beserra Paulo Sanematsu Martin Roffe Leslie D. Kulikowski Felipe D’almeida Costa Tiago G. Santos Glaucia N. M. Hajj |
| author_facet | Fernanda F. da Silva Fernanda C. S. Lupinacci Bruno D. S. Elias Adriano O. Beserra Paulo Sanematsu Martin Roffe Leslie D. Kulikowski Felipe D’almeida Costa Tiago G. Santos Glaucia N. M. Hajj |
| author_sort | Fernanda F. da Silva |
| collection | DOAJ |
| description | Glioblastoma (GBM) is the most common and aggressive primary brain tumor in adults, with few effective treatment strategies. The research on the development of new treatments is often constrained by the limitations of preclinical models, which fail to accurately replicate the disease’s essential characteristics. Herein, we describe the obtention, molecular, and functional characterization of the GBM33 cell line. This cell line belongs to the GBM class according to the World Health Organization 2021 Classification of Central Nervous System Tumors, identified by methylation profiling. GBM33 expresses the astrocytic marker GFAP, as well as markers of neuronal origin commonly expressed in GBM cells, such as βIII-tubulin and neurofilament. Functional assays demonstrated an increased growth rate when compared to the U87 commercial cell line and a similar sensitivity to temozolamide. GBM33 cells retained response to serum starvation, with reduced growth and diminished activation of the Akt signaling pathway. Unlike LN-18 and LN-229 commercial cell lines, GBM33 is able to produce primary cilia upon serum starvation. In summary, the successful establishment and comprehensive characterization of this GBM cell line provide researchers with invaluable tools for studying GBM biology, identifying novel therapeutic targets, and evaluating the efficacy of potential treatments. |
| first_indexed | 2024-03-11T11:28:00Z |
| format | Article |
| id | doaj.art-0701c19ca9f74cf0b89be77ea7e5dec3 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T11:28:00Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0701c19ca9f74cf0b89be77ea7e5dec32023-11-10T15:05:35ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124211586110.3390/ijms242115861Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian PatientFernanda F. da Silva0Fernanda C. S. Lupinacci1Bruno D. S. Elias2Adriano O. Beserra3Paulo Sanematsu4Martin Roffe5Leslie D. Kulikowski6Felipe D’almeida Costa7Tiago G. Santos8Glaucia N. M. Hajj9International Research Center/CIPE, A.C. Camargo Cancer Center, São Paulo 01508-010, BrazilInternational Research Center/CIPE, A.C. Camargo Cancer Center, São Paulo 01508-010, BrazilInternational Research Center/CIPE, A.C. Camargo Cancer Center, São Paulo 01508-010, BrazilInternational Research Center/CIPE, A.C. Camargo Cancer Center, São Paulo 01508-010, BrazilNeurosurgery Department, A.C. Camargo Cancer Center, São Paulo 01509-010, BrazilChildren’s Hospital of Eastern Ontario Research Institute, Ottawa, ON K1H 8L1, CanadaCytogenomics Laboratory, Hospital das Clínicas da Faculdade de Medicina, Universidade de São Paulo, São Paulo 05403-010, BrazilDepartment of Anatomic Pathology, A.C. Camargo Cancer Center, São Paulo 01509-010, BrazilInternational Research Center/CIPE, A.C. Camargo Cancer Center, São Paulo 01508-010, BrazilInternational Research Center/CIPE, A.C. Camargo Cancer Center, São Paulo 01508-010, BrazilGlioblastoma (GBM) is the most common and aggressive primary brain tumor in adults, with few effective treatment strategies. The research on the development of new treatments is often constrained by the limitations of preclinical models, which fail to accurately replicate the disease’s essential characteristics. Herein, we describe the obtention, molecular, and functional characterization of the GBM33 cell line. This cell line belongs to the GBM class according to the World Health Organization 2021 Classification of Central Nervous System Tumors, identified by methylation profiling. GBM33 expresses the astrocytic marker GFAP, as well as markers of neuronal origin commonly expressed in GBM cells, such as βIII-tubulin and neurofilament. Functional assays demonstrated an increased growth rate when compared to the U87 commercial cell line and a similar sensitivity to temozolamide. GBM33 cells retained response to serum starvation, with reduced growth and diminished activation of the Akt signaling pathway. Unlike LN-18 and LN-229 commercial cell lines, GBM33 is able to produce primary cilia upon serum starvation. In summary, the successful establishment and comprehensive characterization of this GBM cell line provide researchers with invaluable tools for studying GBM biology, identifying novel therapeutic targets, and evaluating the efficacy of potential treatments.https://www.mdpi.com/1422-0067/24/21/15861glioblastomacell linesprimary ciliaAktmTORcopy number variation |
| spellingShingle | Fernanda F. da Silva Fernanda C. S. Lupinacci Bruno D. S. Elias Adriano O. Beserra Paulo Sanematsu Martin Roffe Leslie D. Kulikowski Felipe D’almeida Costa Tiago G. Santos Glaucia N. M. Hajj Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian Patient International Journal of Molecular Sciences glioblastoma cell lines primary cilia Akt mTOR copy number variation |
| title | Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian Patient |
| title_full | Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian Patient |
| title_fullStr | Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian Patient |
| title_full_unstemmed | Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian Patient |
| title_short | Establishment and Comprehensive Molecular Characterization of an Immortalized Glioblastoma Cell Line from a Brazilian Patient |
| title_sort | establishment and comprehensive molecular characterization of an immortalized glioblastoma cell line from a brazilian patient |
| topic | glioblastoma cell lines primary cilia Akt mTOR copy number variation |
| url | https://www.mdpi.com/1422-0067/24/21/15861 |
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