The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus
Spodoptera frugiperda (Lepidoptera: Noctuidae) is a major pest in maize crops in Colombia, and affects several regions in America. A granulovirus isolated from S. frugiperda (SfGV VG008) has potential as an enhancer of insecticidal activity of previously described nucleopolyhedrovirus from the same...
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| Format: | Article |
| Language: | English |
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MDPI AG
2015-01-01
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| Series: | Viruses |
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| Online Access: | http://www.mdpi.com/1999-4915/7/1/394 |
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| author | Paola E. Cuartas Gloria P. Barrera Mariano N. Belaich Emiliano Barreto Pablo D. Ghiringhelli Laura F. Villamizar |
| author_facet | Paola E. Cuartas Gloria P. Barrera Mariano N. Belaich Emiliano Barreto Pablo D. Ghiringhelli Laura F. Villamizar |
| author_sort | Paola E. Cuartas |
| collection | DOAJ |
| description | Spodoptera frugiperda (Lepidoptera: Noctuidae) is a major pest in maize crops in Colombia, and affects several regions in America. A granulovirus isolated from S. frugiperda (SfGV VG008) has potential as an enhancer of insecticidal activity of previously described nucleopolyhedrovirus from the same insect species (SfMNPV). The SfGV VG008 genome was sequenced and analyzed showing circular double stranded DNA of 140,913 bp encoding 146 putative ORFs that include 37 Baculoviridae core genes, 88 shared with betabaculoviruses, two shared only with betabaculoviruses from Noctuide insects, two shared with alphabaculoviruses, three copies of own genes (paralogs) and the other 14 corresponding to unique genes without representation in the other baculovirus species. Particularly, the genome encodes for important virulence factors such as 4 chitinases and 2 enhancins. The sequence analysis revealed the existence of eight homologous regions (hrs) and also suggests processes of gene acquisition by horizontal transfer including the SfGV VG008 ORFs 046/047 (paralogs), 059, 089 and 099. The bioinformatics evidence indicates that the genome donors of mentioned genes could be alpha- and/or betabaculovirus species. The previous reported ability of SfGV VG008 to naturally co-infect the same host with other virus show a possible mechanism to capture genes and thus improve its fitness. |
| first_indexed | 2024-04-14T04:46:08Z |
| format | Article |
| id | doaj.art-0703520e957641cf893f78965063c8ca |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-04-14T04:46:08Z |
| publishDate | 2015-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-0703520e957641cf893f78965063c8ca2022-12-22T02:11:27ZengMDPI AGViruses1999-49152015-01-017139442110.3390/v7010394v7010394The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant VirusPaola E. Cuartas0Gloria P. Barrera1Mariano N. Belaich2Emiliano Barreto3Pablo D. Ghiringhelli4Laura F. Villamizar5Centro de investigación Tibaitatá, Corporación Colombiana de Investigación Agropecuaria CORPOICA, Km 14 Vía Mosquera 250047, Cundinamarca, ColombiaCentro de investigación Tibaitatá, Corporación Colombiana de Investigación Agropecuaria CORPOICA, Km 14 Vía Mosquera 250047, Cundinamarca, ColombiaLaboratorio de Ingeniería Genética y Biología Celular y Molecular—Área Virosis de Insectos (LIGBCM—AVI), Dto. de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, Provincia de Buenos Aires, 1876, ArgentinaCentro de Bioinformática, Instituto de Biotecnología, Universidad Nacional de Colombia. Avenida Carrera 30 # 45, Bogotá 11001000, Cundinamarca, ColombiaLaboratorio de Ingeniería Genética y Biología Celular y Molecular—Área Virosis de Insectos (LIGBCM—AVI), Dto. de Ciencia y Tecnología, Universidad Nacional de Quilmes, Roque Saenz Peña 352, Bernal, Provincia de Buenos Aires, 1876, ArgentinaCentro de investigación Tibaitatá, Corporación Colombiana de Investigación Agropecuaria CORPOICA, Km 14 Vía Mosquera 250047, Cundinamarca, ColombiaSpodoptera frugiperda (Lepidoptera: Noctuidae) is a major pest in maize crops in Colombia, and affects several regions in America. A granulovirus isolated from S. frugiperda (SfGV VG008) has potential as an enhancer of insecticidal activity of previously described nucleopolyhedrovirus from the same insect species (SfMNPV). The SfGV VG008 genome was sequenced and analyzed showing circular double stranded DNA of 140,913 bp encoding 146 putative ORFs that include 37 Baculoviridae core genes, 88 shared with betabaculoviruses, two shared only with betabaculoviruses from Noctuide insects, two shared with alphabaculoviruses, three copies of own genes (paralogs) and the other 14 corresponding to unique genes without representation in the other baculovirus species. Particularly, the genome encodes for important virulence factors such as 4 chitinases and 2 enhancins. The sequence analysis revealed the existence of eight homologous regions (hrs) and also suggests processes of gene acquisition by horizontal transfer including the SfGV VG008 ORFs 046/047 (paralogs), 059, 089 and 099. The bioinformatics evidence indicates that the genome donors of mentioned genes could be alpha- and/or betabaculovirus species. The previous reported ability of SfGV VG008 to naturally co-infect the same host with other virus show a possible mechanism to capture genes and thus improve its fitness.http://www.mdpi.com/1999-4915/7/1/394baculovirusgranulovirusSpodoptera frugiperdagenomerecombination |
| spellingShingle | Paola E. Cuartas Gloria P. Barrera Mariano N. Belaich Emiliano Barreto Pablo D. Ghiringhelli Laura F. Villamizar The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus Viruses baculovirus granulovirus Spodoptera frugiperda genome recombination |
| title | The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus |
| title_full | The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus |
| title_fullStr | The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus |
| title_full_unstemmed | The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus |
| title_short | The Complete Sequence of the First Spodoptera frugiperda Betabaculovirus Genome: A Natural Multiple Recombinant Virus |
| title_sort | complete sequence of the first spodoptera frugiperda betabaculovirus genome a natural multiple recombinant virus |
| topic | baculovirus granulovirus Spodoptera frugiperda genome recombination |
| url | http://www.mdpi.com/1999-4915/7/1/394 |
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