Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model
Abstract Background To assess the antibacterial activity of multi-strain probiotics supernatants (MSP); Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus acidophilus as an intracanal medication on Enterococcus faecalis (E. faecalis) biofilm in a tooth model. Methods Sixty extracted...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-04-01
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| Series: | BMC Oral Health |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12903-023-02914-2 |
| _version_ | 1797840764800073728 |
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| author | Shymaa Shaaban Salma Genena Alaaeldin Elraggal Gamal M. Hamad Marwa A. Meheissen Sybel Moussa |
| author_facet | Shymaa Shaaban Salma Genena Alaaeldin Elraggal Gamal M. Hamad Marwa A. Meheissen Sybel Moussa |
| author_sort | Shymaa Shaaban |
| collection | DOAJ |
| description | Abstract Background To assess the antibacterial activity of multi-strain probiotics supernatants (MSP); Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus acidophilus as an intracanal medication on Enterococcus faecalis (E. faecalis) biofilm in a tooth model. Methods Sixty extracted human single-rooted teeth with single canals were instrumented, sterilized, and inoculated with E. faecalis. After 21 days of incubation, four specimens were randomly selected to validate the biofilm formation by scanning electron microscope (SEM). The remaining specimens were randomly divided (n = 14), according to the intracanal medication (ICM) received into: Ca(OH) 2 : calcium hydroxide paste (35% Ultra Cal XS Ca(OH)2), Probiotics supernatants: MSP in poloxamer gel vehicle Poloxamer: poloxamer gel vehicle and, Control: E. faecalis biofilm only. The tested groups were further subdivided into two equal subgroups (n = 7) according to the incubation period (24 h and 7 days). Shaved dentin chips were obtained and collected by H-files and paper points, respectively for bacterial culture. The antibacterial activity was assessed after each incubation period quantitatively and qualitatively using bacterial colony-forming units per milliliter (CFUs/ml) and SEM, respectively. Results The lowest CFUs/ml was found in Ca (OH)2 with a significant difference compared to other groups after 24 h. After 7 days, a similar outcome was found with a further significant reduction of CFUs/ml in all groups with no statistical difference between Ca(OH)2 and probiotics supernatants groups. Ca (OH)2 and Probiotics supernatants groups showed a significant (p < 0.05) percentage of overall bacterial reduction (100.00 ± 0.00% and 70.30 ± 12.95%, respectively) compared to poloxamer and control groups (27.80 ± 14.45 and 28.29 ± 19.79). SEM images showed a bacteria-free state in the Ca(OH)2 group after 7 days while few bacteria were found in the probiotics supernatants group. An extensive invasion of bacteria was found in poloxamer and controls groups. Conclusion MSP has a potential antibacterial effect on E. faecalis growth closely similar to the routinely used Ca (OH)2. |
| first_indexed | 2024-04-09T16:20:00Z |
| format | Article |
| id | doaj.art-0706b2ee9b494e658bced828e05df1d7 |
| institution | Directory Open Access Journal |
| issn | 1472-6831 |
| language | English |
| last_indexed | 2024-04-09T16:20:00Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Oral Health |
| spelling | doaj.art-0706b2ee9b494e658bced828e05df1d72023-04-23T11:30:13ZengBMCBMC Oral Health1472-68312023-04-0123111110.1186/s12903-023-02914-2Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth modelShymaa Shaaban0Salma Genena1Alaaeldin Elraggal2Gamal M. Hamad3Marwa A. Meheissen4Sybel Moussa5Division of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria UniversityDivision of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria UniversityDivision of Operative Dentistry, Conservative Dentistry Department, Faculty of Dentistry, Alexandria UniversityDepartment of Food Technology, Arid Lands Cultivation Research Institute (ALCRI), City of Scientific Research and Technological Applications (SRTACity)Medical Microbiology & Immunology Department, Faculty of Medicine, Alexandria UniversityDivision of Endodontics, Conservative Dentistry Department, Faculty of Dentistry, Alexandria UniversityAbstract Background To assess the antibacterial activity of multi-strain probiotics supernatants (MSP); Lactobacillus plantarum, Lactobacillus rhamnosus, and Lactobacillus acidophilus as an intracanal medication on Enterococcus faecalis (E. faecalis) biofilm in a tooth model. Methods Sixty extracted human single-rooted teeth with single canals were instrumented, sterilized, and inoculated with E. faecalis. After 21 days of incubation, four specimens were randomly selected to validate the biofilm formation by scanning electron microscope (SEM). The remaining specimens were randomly divided (n = 14), according to the intracanal medication (ICM) received into: Ca(OH) 2 : calcium hydroxide paste (35% Ultra Cal XS Ca(OH)2), Probiotics supernatants: MSP in poloxamer gel vehicle Poloxamer: poloxamer gel vehicle and, Control: E. faecalis biofilm only. The tested groups were further subdivided into two equal subgroups (n = 7) according to the incubation period (24 h and 7 days). Shaved dentin chips were obtained and collected by H-files and paper points, respectively for bacterial culture. The antibacterial activity was assessed after each incubation period quantitatively and qualitatively using bacterial colony-forming units per milliliter (CFUs/ml) and SEM, respectively. Results The lowest CFUs/ml was found in Ca (OH)2 with a significant difference compared to other groups after 24 h. After 7 days, a similar outcome was found with a further significant reduction of CFUs/ml in all groups with no statistical difference between Ca(OH)2 and probiotics supernatants groups. Ca (OH)2 and Probiotics supernatants groups showed a significant (p < 0.05) percentage of overall bacterial reduction (100.00 ± 0.00% and 70.30 ± 12.95%, respectively) compared to poloxamer and control groups (27.80 ± 14.45 and 28.29 ± 19.79). SEM images showed a bacteria-free state in the Ca(OH)2 group after 7 days while few bacteria were found in the probiotics supernatants group. An extensive invasion of bacteria was found in poloxamer and controls groups. Conclusion MSP has a potential antibacterial effect on E. faecalis growth closely similar to the routinely used Ca (OH)2.https://doi.org/10.1186/s12903-023-02914-2ProbioticsLactobacillusIntracanal medicationEnterococcus faecalis biofilmCalcium hydroxide |
| spellingShingle | Shymaa Shaaban Salma Genena Alaaeldin Elraggal Gamal M. Hamad Marwa A. Meheissen Sybel Moussa Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model BMC Oral Health Probiotics Lactobacillus Intracanal medication Enterococcus faecalis biofilm Calcium hydroxide |
| title | Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model |
| title_full | Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model |
| title_fullStr | Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model |
| title_full_unstemmed | Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model |
| title_short | Antibacterial effectiveness of multi-strain probiotics supernatants intracanal medication on Enterococcus faecalis biofilm in a tooth model |
| title_sort | antibacterial effectiveness of multi strain probiotics supernatants intracanal medication on enterococcus faecalis biofilm in a tooth model |
| topic | Probiotics Lactobacillus Intracanal medication Enterococcus faecalis biofilm Calcium hydroxide |
| url | https://doi.org/10.1186/s12903-023-02914-2 |
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