Estimation of alpha-amylase in smokers with and without leukoplakia and oral cancer—A comparative study
Background: Cigarette smoke releases free radicles that have deleterious effects on the oral mucosa including potentially malignant disorders and oral cancer. In response to cigarette smoking, salivary flow rate increases as a defense activity along with a rise in alpha-amylase levels. Aim: To estim...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2023-01-01
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| Series: | Journal of Indian Academy of Oral Medicine and Radiology |
| Subjects: | |
| Online Access: | http://www.jiaomr.in/article.asp?issn=0972-1363;year=2023;volume=35;issue=3;spage=331;epage=334;aulast=Akkinepally |
| _version_ | 1797645611838734336 |
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| author | Harshitha Akkinepally M L Avinash Tejasvi N Lakshmi Kavitha Archana Pokala Praveen Reddy Vummadi Reddy Keerthi |
| author_facet | Harshitha Akkinepally M L Avinash Tejasvi N Lakshmi Kavitha Archana Pokala Praveen Reddy Vummadi Reddy Keerthi |
| author_sort | Harshitha Akkinepally |
| collection | DOAJ |
| description | Background: Cigarette smoke releases free radicles that have deleterious effects on the oral mucosa including potentially malignant disorders and oral cancer. In response to cigarette smoking, salivary flow rate increases as a defense activity along with a rise in alpha-amylase levels. Aim: To estimate salivary alpha-amylase levels in smokers with and without leukoplakia and oral cancer cases. Methods: This comparative study was carried out on 120 subjects; 30 subjects each in four groups which include non-smokers, smokers without oral lesions, smokers with leukoplakia, and smokers with oral squamous cell carcinoma (OSCC). Five microliters of unstimulated saliva were collected from the subjects by the spitting method. Alpha-amylase salivary levels were determined by spectrophotometric assay. Data collected were analyzed using a one-way ANOVA test. Results: No significant difference between alpha-amylase values was observed among smokers without oral lesions and smokers with leukoplakia groups when compared with non-smokers, but a significant difference was seen in the OSCC group in comparison with all three groups (smokers without oral lesions group vs OSCC group – P = 0.0045; leukoplakia group vs OSCC group P = 0.0326). Conclusion: Salivary alpha-amylase levels increased in smokers without any lesion and smokers with leukoplakia when compared with nonsmokers, although it was statistically not significant. Salivary alpha-amylase levels in oral cancer were significantly higher when compared with non-smokers, and smokers with and without leukoplakia. Smoking induced a change in salivary antioxidant levels. Changes in antioxidant levels can influence the deleterious effects of smoking on oral mucosa as well as systemic health. |
| first_indexed | 2024-03-11T14:49:21Z |
| format | Article |
| id | doaj.art-0707f7bdf52e428a939766039285514a |
| institution | Directory Open Access Journal |
| issn | 0972-1363 |
| language | English |
| last_indexed | 2024-03-11T14:49:21Z |
| publishDate | 2023-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Indian Academy of Oral Medicine and Radiology |
| spelling | doaj.art-0707f7bdf52e428a939766039285514a2023-10-30T10:03:33ZengWolters Kluwer Medknow PublicationsJournal of Indian Academy of Oral Medicine and Radiology0972-13632023-01-0135333133410.4103/jiaomr.jiaomr_230_23Estimation of alpha-amylase in smokers with and without leukoplakia and oral cancer—A comparative studyHarshitha AkkinepallyM L Avinash TejasviN Lakshmi KavithaArchana PokalaPraveen ReddyVummadi Reddy KeerthiBackground: Cigarette smoke releases free radicles that have deleterious effects on the oral mucosa including potentially malignant disorders and oral cancer. In response to cigarette smoking, salivary flow rate increases as a defense activity along with a rise in alpha-amylase levels. Aim: To estimate salivary alpha-amylase levels in smokers with and without leukoplakia and oral cancer cases. Methods: This comparative study was carried out on 120 subjects; 30 subjects each in four groups which include non-smokers, smokers without oral lesions, smokers with leukoplakia, and smokers with oral squamous cell carcinoma (OSCC). Five microliters of unstimulated saliva were collected from the subjects by the spitting method. Alpha-amylase salivary levels were determined by spectrophotometric assay. Data collected were analyzed using a one-way ANOVA test. Results: No significant difference between alpha-amylase values was observed among smokers without oral lesions and smokers with leukoplakia groups when compared with non-smokers, but a significant difference was seen in the OSCC group in comparison with all three groups (smokers without oral lesions group vs OSCC group – P = 0.0045; leukoplakia group vs OSCC group P = 0.0326). Conclusion: Salivary alpha-amylase levels increased in smokers without any lesion and smokers with leukoplakia when compared with nonsmokers, although it was statistically not significant. Salivary alpha-amylase levels in oral cancer were significantly higher when compared with non-smokers, and smokers with and without leukoplakia. Smoking induced a change in salivary antioxidant levels. Changes in antioxidant levels can influence the deleterious effects of smoking on oral mucosa as well as systemic health.http://www.jiaomr.in/article.asp?issn=0972-1363;year=2023;volume=35;issue=3;spage=331;epage=334;aulast=Akkinepallyalpha-amylaseoral canceroral leukoplakiasmoking |
| spellingShingle | Harshitha Akkinepally M L Avinash Tejasvi N Lakshmi Kavitha Archana Pokala Praveen Reddy Vummadi Reddy Keerthi Estimation of alpha-amylase in smokers with and without leukoplakia and oral cancer—A comparative study Journal of Indian Academy of Oral Medicine and Radiology alpha-amylase oral cancer oral leukoplakia smoking |
| title | Estimation of alpha-amylase in smokers with and without leukoplakia and oral cancer—A comparative study |
| title_full | Estimation of alpha-amylase in smokers with and without leukoplakia and oral cancer—A comparative study |
| title_fullStr | Estimation of alpha-amylase in smokers with and without leukoplakia and oral cancer—A comparative study |
| title_full_unstemmed | Estimation of alpha-amylase in smokers with and without leukoplakia and oral cancer—A comparative study |
| title_short | Estimation of alpha-amylase in smokers with and without leukoplakia and oral cancer—A comparative study |
| title_sort | estimation of alpha amylase in smokers with and without leukoplakia and oral cancer a comparative study |
| topic | alpha-amylase oral cancer oral leukoplakia smoking |
| url | http://www.jiaomr.in/article.asp?issn=0972-1363;year=2023;volume=35;issue=3;spage=331;epage=334;aulast=Akkinepally |
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