Variations in the Mycorrhization Characteristics in Roots of Wild-Type and ABA-Deficient Tomato Are Accompanied by Specific Transcriptomic Alterations
Abscissic acid (ABA) determines mycorrhiza functionality and arbuscule development. In this study, we performed transcriptome analysis in response to different mycorrhization status according to the ABA content in the root to identify genes that may play a role in arbuscule functionality. Affymetrix...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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The American Phytopathological Society
2010-05-01
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| Series: | Molecular Plant-Microbe Interactions |
| Online Access: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-5-0651 |
| _version_ | 1828260161405321216 |
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| author | José Manuel García Garrido Rafael Jorge León Morcillo José Ángel Martín Rodríguez Juan Antonio Ocampo Bote |
| author_facet | José Manuel García Garrido Rafael Jorge León Morcillo José Ángel Martín Rodríguez Juan Antonio Ocampo Bote |
| author_sort | José Manuel García Garrido |
| collection | DOAJ |
| description | Abscissic acid (ABA) determines mycorrhiza functionality and arbuscule development. In this study, we performed transcriptome analysis in response to different mycorrhization status according to the ABA content in the root to identify genes that may play a role in arbuscule functionality. Affymetrix Tomato GeneChip (approximately 10,000 probes) allowed us to detect and compare the transcriptional root profiling of tomato (Solanum lycopersicum) wild-type and ABA-deficient sitiens plants colonized by Glomus intraradices. A number of identified genes in tomato belong to a category of genes already described as “mycorrhizal core-set” in other host plants. The impairment in arbuscular mycorrhiza (AM) formation in ABA-deficient mutants was associated with upregulation of genes related to defense and cell wall modification, whereas functional mycorrhization in wild-type plants was associated with activation of genes related to isoprenoid metabolism. The oxylipin pathway was activated in tomato mycorrhizal roots at late stages of interaction, and was related to the control of fungal spread in roots, not with the establishment of the symbiosis. Induction of selected genes, representing a range of biological functions and representative of the three sets of genes specifically upregulated in the different plant phenotype, was confirmed by quantitative reverse-transcription polymerase chain reaction, and their response to phythohormone treatment was tested, showing that ethylene and jasmonic acid are key regulators of gene expression during AM development. Comparative analysis of mycorrhiza upregulated functional categories revealed significant changes in gene expression associated with the different mycorrhization status according to the ABA content in the roots. |
| first_indexed | 2024-04-13T03:18:08Z |
| format | Article |
| id | doaj.art-07082b0b70e4478a92e17b13160e7e90 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:18:08Z |
| publishDate | 2010-05-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-07082b0b70e4478a92e17b13160e7e902022-12-22T03:04:50ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062010-05-0123565166410.1094/MPMI-23-5-0651Variations in the Mycorrhization Characteristics in Roots of Wild-Type and ABA-Deficient Tomato Are Accompanied by Specific Transcriptomic AlterationsJosé Manuel García GarridoRafael Jorge León MorcilloJosé Ángel Martín RodríguezJuan Antonio Ocampo BoteAbscissic acid (ABA) determines mycorrhiza functionality and arbuscule development. In this study, we performed transcriptome analysis in response to different mycorrhization status according to the ABA content in the root to identify genes that may play a role in arbuscule functionality. Affymetrix Tomato GeneChip (approximately 10,000 probes) allowed us to detect and compare the transcriptional root profiling of tomato (Solanum lycopersicum) wild-type and ABA-deficient sitiens plants colonized by Glomus intraradices. A number of identified genes in tomato belong to a category of genes already described as “mycorrhizal core-set” in other host plants. The impairment in arbuscular mycorrhiza (AM) formation in ABA-deficient mutants was associated with upregulation of genes related to defense and cell wall modification, whereas functional mycorrhization in wild-type plants was associated with activation of genes related to isoprenoid metabolism. The oxylipin pathway was activated in tomato mycorrhizal roots at late stages of interaction, and was related to the control of fungal spread in roots, not with the establishment of the symbiosis. Induction of selected genes, representing a range of biological functions and representative of the three sets of genes specifically upregulated in the different plant phenotype, was confirmed by quantitative reverse-transcription polymerase chain reaction, and their response to phythohormone treatment was tested, showing that ethylene and jasmonic acid are key regulators of gene expression during AM development. Comparative analysis of mycorrhiza upregulated functional categories revealed significant changes in gene expression associated with the different mycorrhization status according to the ABA content in the roots.https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-5-0651 |
| spellingShingle | José Manuel García Garrido Rafael Jorge León Morcillo José Ángel Martín Rodríguez Juan Antonio Ocampo Bote Variations in the Mycorrhization Characteristics in Roots of Wild-Type and ABA-Deficient Tomato Are Accompanied by Specific Transcriptomic Alterations Molecular Plant-Microbe Interactions |
| title | Variations in the Mycorrhization Characteristics in Roots of Wild-Type and ABA-Deficient Tomato Are Accompanied by Specific Transcriptomic Alterations |
| title_full | Variations in the Mycorrhization Characteristics in Roots of Wild-Type and ABA-Deficient Tomato Are Accompanied by Specific Transcriptomic Alterations |
| title_fullStr | Variations in the Mycorrhization Characteristics in Roots of Wild-Type and ABA-Deficient Tomato Are Accompanied by Specific Transcriptomic Alterations |
| title_full_unstemmed | Variations in the Mycorrhization Characteristics in Roots of Wild-Type and ABA-Deficient Tomato Are Accompanied by Specific Transcriptomic Alterations |
| title_short | Variations in the Mycorrhization Characteristics in Roots of Wild-Type and ABA-Deficient Tomato Are Accompanied by Specific Transcriptomic Alterations |
| title_sort | variations in the mycorrhization characteristics in roots of wild type and aba deficient tomato are accompanied by specific transcriptomic alterations |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-23-5-0651 |
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