The Antimicrobial Resistance Characteristics of Imipenem-Non-Susceptible, Imipenemase-6-Producing <i>Escherichia coli</i>
Imipenemase-6 (IMP-6) type carbapenemase-producing <i>Enterobacteriaceae</i> is regarded as dangerous due to its unique lack of antimicrobial susceptibility. It is resistant to meropenem (MEPM) but susceptible to imipenem (IPM). In addition to carbapenemase, outer membrane porins and eff...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/11/1/32 |
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| author | Reo Onishi Katsumi Shigemura Kayo Osawa Young-Min Yang Koki Maeda Shiuh-Bin Fang Shian-Ying Sung Kenichiro Onuma Atsushi Uda Takayuki Miyara Masato Fujisawa |
| author_facet | Reo Onishi Katsumi Shigemura Kayo Osawa Young-Min Yang Koki Maeda Shiuh-Bin Fang Shian-Ying Sung Kenichiro Onuma Atsushi Uda Takayuki Miyara Masato Fujisawa |
| author_sort | Reo Onishi |
| collection | DOAJ |
| description | Imipenemase-6 (IMP-6) type carbapenemase-producing <i>Enterobacteriaceae</i> is regarded as dangerous due to its unique lack of antimicrobial susceptibility. It is resistant to meropenem (MEPM) but susceptible to imipenem (IPM). In addition to carbapenemase, outer membrane porins and efflux pumps also play roles in carbapenem resistance by reducing the antimicrobial concentration inside cells. Extended-spectrum β-lactamase (ESBL) is transmitted with IMP-6 by the plasmid and broadens the spectrum of antimicrobial resistance. We collected 42 strains of IMP-6-producing <i>Escherichia coli</i> and conducted a molecular analysis of carbapenemase, ESBL, porin, efflux, and epidemiological characteristics using plasmid replicon typing. Among the 42 isolates, 21 strains were susceptible to IPM (50.0%) and 1 (2.4%) to MEPM. Seventeen strains (40.5%) co-produced CTX-M-2 type ESBL. We found that the relative expression of <i>ompC</i> and <i>ompF</i> significantly correlated with the MIC of IPM (<i>p</i> = 0.01 and <i>p</i> = 0.03, respectively). Sixty-eight% of CTX-M-2-non-producing strains had IncI1, which was significantly different from CTX-M-2-producing strains (<i>p</i> < 0.001). In conclusion, 50.0% of our IMP-6-producing strains were non-susceptible to IPM, which is different from the typical pattern and can be attributed to decreased porin expression. Further studies investigating other types of carbapenemase are warranted. |
| first_indexed | 2024-03-10T03:02:21Z |
| format | Article |
| id | doaj.art-070b6ae7431b4294a0488af26d13777b |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T03:02:21Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-070b6ae7431b4294a0488af26d13777b2023-11-23T12:42:58ZengMDPI AGAntibiotics2079-63822021-12-011113210.3390/antibiotics11010032The Antimicrobial Resistance Characteristics of Imipenem-Non-Susceptible, Imipenemase-6-Producing <i>Escherichia coli</i>Reo Onishi0Katsumi Shigemura1Kayo Osawa2Young-Min Yang3Koki Maeda4Shiuh-Bin Fang5Shian-Ying Sung6Kenichiro Onuma7Atsushi Uda8Takayuki Miyara9Masato Fujisawa10Department of Public Health, Division of Infectious Diseases, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka Suma-ku, Kobe 654-0142, JapanDepartment of Public Health, Division of Infectious Diseases, Kobe University Graduate School of Health Sciences, 7-10-2 Tomogaoka Suma-ku, Kobe 654-0142, JapanDepartment of Medical Technology, Kobe Tokiwa University, 2-6-2 Otani-cho, Nagata-ku, Kobe 653-0838, JapanDivision of Urology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Urology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDepartment of Pediatrics, Division of Pediatric Gastroenterology and Hepatology, Shuang Ho Hospital, Taipei Medical University, 291 Jhong Jheng Road, Jhong Ho District, New Taipei City 23561, TaiwanInternational Ph.D. Program for Translational Science, College of Medical Science and Technology, Taipei Medical University, Taipei 11031, TaiwanDepartment of Infection Control and Prevention, Kobe University Hospital, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDepartment of Infection Control and Prevention, Kobe University Hospital, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDepartment of Infection Control and Prevention, Kobe University Hospital, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Urology, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanImipenemase-6 (IMP-6) type carbapenemase-producing <i>Enterobacteriaceae</i> is regarded as dangerous due to its unique lack of antimicrobial susceptibility. It is resistant to meropenem (MEPM) but susceptible to imipenem (IPM). In addition to carbapenemase, outer membrane porins and efflux pumps also play roles in carbapenem resistance by reducing the antimicrobial concentration inside cells. Extended-spectrum β-lactamase (ESBL) is transmitted with IMP-6 by the plasmid and broadens the spectrum of antimicrobial resistance. We collected 42 strains of IMP-6-producing <i>Escherichia coli</i> and conducted a molecular analysis of carbapenemase, ESBL, porin, efflux, and epidemiological characteristics using plasmid replicon typing. Among the 42 isolates, 21 strains were susceptible to IPM (50.0%) and 1 (2.4%) to MEPM. Seventeen strains (40.5%) co-produced CTX-M-2 type ESBL. We found that the relative expression of <i>ompC</i> and <i>ompF</i> significantly correlated with the MIC of IPM (<i>p</i> = 0.01 and <i>p</i> = 0.03, respectively). Sixty-eight% of CTX-M-2-non-producing strains had IncI1, which was significantly different from CTX-M-2-producing strains (<i>p</i> < 0.001). In conclusion, 50.0% of our IMP-6-producing strains were non-susceptible to IPM, which is different from the typical pattern and can be attributed to decreased porin expression. Further studies investigating other types of carbapenemase are warranted.https://www.mdpi.com/2079-6382/11/1/32IMP-6carbapenemaseporinefflux pumpplasmid |
| spellingShingle | Reo Onishi Katsumi Shigemura Kayo Osawa Young-Min Yang Koki Maeda Shiuh-Bin Fang Shian-Ying Sung Kenichiro Onuma Atsushi Uda Takayuki Miyara Masato Fujisawa The Antimicrobial Resistance Characteristics of Imipenem-Non-Susceptible, Imipenemase-6-Producing <i>Escherichia coli</i> Antibiotics IMP-6 carbapenemase porin efflux pump plasmid |
| title | The Antimicrobial Resistance Characteristics of Imipenem-Non-Susceptible, Imipenemase-6-Producing <i>Escherichia coli</i> |
| title_full | The Antimicrobial Resistance Characteristics of Imipenem-Non-Susceptible, Imipenemase-6-Producing <i>Escherichia coli</i> |
| title_fullStr | The Antimicrobial Resistance Characteristics of Imipenem-Non-Susceptible, Imipenemase-6-Producing <i>Escherichia coli</i> |
| title_full_unstemmed | The Antimicrobial Resistance Characteristics of Imipenem-Non-Susceptible, Imipenemase-6-Producing <i>Escherichia coli</i> |
| title_short | The Antimicrobial Resistance Characteristics of Imipenem-Non-Susceptible, Imipenemase-6-Producing <i>Escherichia coli</i> |
| title_sort | antimicrobial resistance characteristics of imipenem non susceptible imipenemase 6 producing i escherichia coli i |
| topic | IMP-6 carbapenemase porin efflux pump plasmid |
| url | https://www.mdpi.com/2079-6382/11/1/32 |
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