Development and verification of a prognostic model for colon cancer on pyroptosis-related genes
Background: Recently, the role of pyroptosis in cancer has attracted people’s attention, but its function in colon cancer remains unclear. This study aimed to construct a pyroptosis-related model that can effectively predict the prognosis of patients with colon cancer and explore the potential funct...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.922055/full |
| _version_ | 1797997670937133056 |
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| author | Zizhen Wu Zizhen Wu Bo Wang Bo Wang Yingjiang Ye Yingjiang Ye Shan Wang Shan Wang Kewei Jiang Kewei Jiang |
| author_facet | Zizhen Wu Zizhen Wu Bo Wang Bo Wang Yingjiang Ye Yingjiang Ye Shan Wang Shan Wang Kewei Jiang Kewei Jiang |
| author_sort | Zizhen Wu |
| collection | DOAJ |
| description | Background: Recently, the role of pyroptosis in cancer has attracted people’s attention, but its function in colon cancer remains unclear. This study aimed to construct a pyroptosis-related model that can effectively predict the prognosis of patients with colon cancer and explore the potential functions of pyroptosis-related genes.Methods: We identified 40 differentially expressed PRGs between colon cancer and normal colon tissues. The model was established using the least absolute shrinkage and selection operator (LASSO) Cox regression method, and the patients were divided into high- and low-risk groups. Finally, we verified its biological function in vitro based on three PRGs and demonstrated discrepant expression of PRGs within colon cancer and non-tumor tissues at the protein level with immunohistochemistry.Results: A pyroptosis-related prognosis model was constructed, which divided 446 patients with colon cancer into high- and low-risk groups. Kaplan–Meier analysis results showed that the survival of patients with colon cancer in the high-risk group was worse than that in the low-risk group. Finally, we also confirmed that this score is an independent prognostic factor for colon cancer progression.Conclusion: In summary, the model established by three PRGs was a reliable indicator for predicting prognosis, suggesting that pyroptosis might be a noteworthy therapeutic target in CC. |
| first_indexed | 2024-04-11T10:36:26Z |
| format | Article |
| id | doaj.art-070cd6e89ad14ff588603c12f5058af6 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-04-11T10:36:26Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-070cd6e89ad14ff588603c12f5058af62022-12-22T04:29:17ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-09-011310.3389/fgene.2022.922055922055Development and verification of a prognostic model for colon cancer on pyroptosis-related genesZizhen Wu0Zizhen Wu1Bo Wang2Bo Wang3Yingjiang Ye4Yingjiang Ye5Shan Wang6Shan Wang7Kewei Jiang8Kewei Jiang9Department of Gastroenterological Surgery, Peking University People’s Hospital, Beijing, ChinaLaboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People’s Hospital, Beijing, ChinaDepartment of Gastroenterological Surgery, Peking University People’s Hospital, Beijing, ChinaLaboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People’s Hospital, Beijing, ChinaDepartment of Gastroenterological Surgery, Peking University People’s Hospital, Beijing, ChinaLaboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People’s Hospital, Beijing, ChinaDepartment of Gastroenterological Surgery, Peking University People’s Hospital, Beijing, ChinaLaboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People’s Hospital, Beijing, ChinaDepartment of Gastroenterological Surgery, Peking University People’s Hospital, Beijing, ChinaLaboratory of Surgical Oncology, Beijing Key Laboratory of Colorectal Cancer Diagnosis and Treatment Research, Peking University People’s Hospital, Beijing, ChinaBackground: Recently, the role of pyroptosis in cancer has attracted people’s attention, but its function in colon cancer remains unclear. This study aimed to construct a pyroptosis-related model that can effectively predict the prognosis of patients with colon cancer and explore the potential functions of pyroptosis-related genes.Methods: We identified 40 differentially expressed PRGs between colon cancer and normal colon tissues. The model was established using the least absolute shrinkage and selection operator (LASSO) Cox regression method, and the patients were divided into high- and low-risk groups. Finally, we verified its biological function in vitro based on three PRGs and demonstrated discrepant expression of PRGs within colon cancer and non-tumor tissues at the protein level with immunohistochemistry.Results: A pyroptosis-related prognosis model was constructed, which divided 446 patients with colon cancer into high- and low-risk groups. Kaplan–Meier analysis results showed that the survival of patients with colon cancer in the high-risk group was worse than that in the low-risk group. Finally, we also confirmed that this score is an independent prognostic factor for colon cancer progression.Conclusion: In summary, the model established by three PRGs was a reliable indicator for predicting prognosis, suggesting that pyroptosis might be a noteworthy therapeutic target in CC.https://www.frontiersin.org/articles/10.3389/fgene.2022.922055/fullcolon cancerbioinformatics analysispyroptosisprognosissurvival |
| spellingShingle | Zizhen Wu Zizhen Wu Bo Wang Bo Wang Yingjiang Ye Yingjiang Ye Shan Wang Shan Wang Kewei Jiang Kewei Jiang Development and verification of a prognostic model for colon cancer on pyroptosis-related genes Frontiers in Genetics colon cancer bioinformatics analysis pyroptosis prognosis survival |
| title | Development and verification of a prognostic model for colon cancer on pyroptosis-related genes |
| title_full | Development and verification of a prognostic model for colon cancer on pyroptosis-related genes |
| title_fullStr | Development and verification of a prognostic model for colon cancer on pyroptosis-related genes |
| title_full_unstemmed | Development and verification of a prognostic model for colon cancer on pyroptosis-related genes |
| title_short | Development and verification of a prognostic model for colon cancer on pyroptosis-related genes |
| title_sort | development and verification of a prognostic model for colon cancer on pyroptosis related genes |
| topic | colon cancer bioinformatics analysis pyroptosis prognosis survival |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.922055/full |
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